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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-mouse CD3ε
catalog :
100325
quantity :
25 μg
price :
88 USD
clonality :
monoclonal
host :
hamsters
conjugate :
PerCP
clone name :
145-2C11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 57
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse
Pérez Girón J, Gómez Medina S, Lüdtke A, Munoz Fontela C. Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity. J Vis Exp. 2015;:e52803 pubmed publisher
  • flow cytometry; mouse; fig 2
Stack G, Jones E, Marsden M, Stacey M, Snelgrove R, Lacaze P, et al. CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue. PLoS Pathog. 2015;11:e1004641 pubmed publisher
Chen F, Huang Y, Huang Z, Jiang T, Yang Z, Zeng J, et al. DNA-scaffolded multivalent vaccine against SARS-CoV-2. Acta Biomater. 2023;164:387-396 pubmed publisher
Li X, Li Y, Dong L, Chang Y, Zhang X, Wang C, et al. Decitabine priming increases anti-PD-1 antitumor efficacy by promoting CD8+ progenitor exhausted T cell expansion in tumor models. J Clin Invest. 2023;133: pubmed publisher
Nakao R, Kobayashi H, Iwabuchi Y, Kawahara K, Hirayama S, Ramstedt M, et al. A highly immunogenic vaccine platform against encapsulated pathogens using chimeric probiotic Escherichia coli membrane vesicles. NPJ Vaccines. 2022;7:153 pubmed publisher
Zhang H, Liu S, Li Y, Li J, Ni C, Yang M, et al. Dysfunction of S100A4+ effector memory CD8+ T cells aggravates asthma. Eur J Immunol. 2022;52:978-993 pubmed publisher
Bettke J, Tam J, Montoya V, Butler B, van der Velden A. Inflammatory Monocytes Promote Granuloma-Mediated Control of Persistent Salmonella Infection. Infect Immun. 2022;90:e0007022 pubmed publisher
Ajay A, Zhao L, Vig S, Fujiwara M, Thakurela S, Jadhav S, et al. Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration. Cell Rep. 2022;38:110473 pubmed publisher
Daneshmandi S, Cassel T, Higashi R, Fan T, Seth P. 6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function. elife. 2021;10: pubmed publisher
Markov S, Caffrey T, O Connell K, Grunkemeyer J, Shin S, Hanson R, et al. IgE-Based Therapeutic Combination Enhances Antitumor Response in Preclinical Models of Pancreatic Cancer. Mol Cancer Ther. 2021;20:2457-2468 pubmed publisher
Aguilera M, Rossini V, Hickey A, Simnica D, Grady F, Felice V, et al. Inflammasome Signaling Regulates the Microbial-Neuroimmune Axis and Visceral Pain in Mice. Int J Mol Sci. 2021;22: pubmed publisher
AbuEid M, McAllister D, McOlash L, Harwig M, Cheng G, Drouillard D, et al. Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression. iScience. 2021;24:102653 pubmed publisher
Terp M, Gammelgaard O, Vever H, Gjerstorff M, Ditzel H. Sustained compensatory p38 MAPK signaling following treatment with MAPK inhibitors induces the immunosuppressive protein CD73 in cancer: combined targeting could improve outcomes. Mol Oncol. 2021;15:3299-3316 pubmed publisher
Murdock B, Famie J, Piecuch C, Raue K, Mendelson F, Pieroni C, et al. NK cells associate with ALS in a sex- and age-dependent manner. JCI Insight. 2021;6: pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Ma Y, Wu T, Ling C, Yu F, Zhang J, Cao P, et al. M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B. Mol Ther Oncolytics. 2021;20:484-498 pubmed publisher
Daneshmandi S, Cassel T, Lin P, Higashi R, Wulf G, Boussiotis V, et al. Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function. Cell Rep. 2021;34:108831 pubmed publisher
Wang L, Chiu S, Lee W, Siu L, Liu K, Yen M, et al. Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella. STAR Protoc. 2021;2:100337 pubmed publisher
Li Z, Suo B, Long G, Gao Y, Song J, Zhang M, et al. Exosomal miRNA-16-5p Derived From M1 Macrophages Enhances T Cell-Dependent Immune Response by Regulating PD-L1 in Gastric Cancer. Front Cell Dev Biol. 2020;8:572689 pubmed publisher
Ferrere G, Tidjani Alou M, Liu P, Goubet A, Fidelle M, Kepp O, et al. Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade. JCI Insight. 2021;6: pubmed publisher
Wang L, Wang H, Chiang H, Huang L, Chiu S, Siu L, et al. Human Placental MSC-Secreted IL-1β Enhances Neutrophil Bactericidal Functions during Hypervirulent Klebsiella Infection. Cell Rep. 2020;32:108188 pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
Li P, Ge D, Li P, Hu F, Chu J, Chen X, et al. CXXC finger protein 4 inhibits the CDK18-ERK1/2 axis to suppress the immune escape of gastric cancer cells with involvement of ELK1/MIR100HG pathway. J Cell Mol Med. 2020;24:10151-10165 pubmed publisher
Xu X, Feng X, He M, Zhang Z, Wang J, Zhu H, et al. The effect of acupuncture on tumor growth and gut microbiota in mice inoculated with osteosarcoma cells. Chin Med. 2020;15:33 pubmed publisher
Tian T, Lofftus S, Pan Y, Stingley C, King S, Zhao J, et al. IL1α Antagonizes IL1β and Promotes Adaptive Immune Rejection of Malignant Tumors. Cancer Immunol Res. 2020;8:660-671 pubmed publisher
Koh C, Kim I, Shin K, Jeon I, Song B, Lee J, et al. GITR Agonism Triggers Antitumor Immune Responses through IL21-Expressing Follicular Helper T Cells. Cancer Immunol Res. 2020;8:698-709 pubmed publisher
Schreiber K, Karrison T, Wolf S, Kiyotani K, Steiner M, Littmann E, et al. Impact of TCR Diversity on the Development of Transplanted or Chemically Induced Tumors. Cancer Immunol Res. 2020;8:192-202 pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
Cha J, Yang W, Xia W, Wei Y, Chan L, Lim S, et al. Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1. Mol Cell. 2018;71:606-620.e7 pubmed publisher
Lino A, Dang V, Lampropoulou V, Welle A, Joedicke J, Pohar J, et al. LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells. Immunity. 2018;49:120-133.e9 pubmed publisher
Iwamoto H, Abe M, Yang Y, Cui D, Seki T, Nakamura M, et al. Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance. Cell Metab. 2018;28:104-117.e5 pubmed publisher
Hsu J, Xia W, Hsu Y, Chan L, Yu W, Cha J, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018;9:1908 pubmed publisher
Oyarce C, Cruz Gómez S, Gálvez Cancino F, Vargas P, Moreau H, Diaz Valdivia N, et al. Caveolin-1 Expression Increases upon Maturation in Dendritic Cells and Promotes Their Migration to Lymph Nodes Thereby Favoring the Induction of CD8+ T Cell Responses. Front Immunol. 2017;8:1794 pubmed publisher
Neumann A, Abele J, Kirschstein T, Engelmann R, Sellmann T, Köhling R, et al. Mycophenolate mofetil prevents the delayed T cell response after pilocarpine-induced status epilepticus in mice. PLoS ONE. 2017;12:e0187330 pubmed publisher
Li X, Zhang X, Gong P, Xia F, Li L, Yang Z, et al. TLR2-/- Mice Display Decreased Severity of Giardiasis via Enhanced Proinflammatory Cytokines Production Dependent on AKT Signal Pathway. Front Immunol. 2017;8:1186 pubmed publisher
Arima Y, Ohki T, Nishikawa N, Higuchi K, Ota M, Tanaka Y, et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. elife. 2017;6: pubmed publisher
Fasbender F, Claus M, Wingert S, Sandusky M, Watzl C. Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes. Front Immunol. 2017;8:789 pubmed publisher
Cabrera Mora M, Fonseca J, Singh B, Zhao C, Makarova N, Dmitriev I, et al. A Recombinant Chimeric Ad5/3 Vector Expressing a Multistage Plasmodium Antigen Induces Protective Immunity in Mice Using Heterologous Prime-Boost Immunization Regimens. J Immunol. 2016;197:2748-61 pubmed publisher
Ito C, Okuyama Dobashi K, Miyasaka T, Masuda C, Sato M, Kawano T, et al. CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma. PLoS ONE. 2015;10:e0140808 pubmed publisher
Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
Könnecke I, Serra A, El Khassawna T, Schlundt C, Schell H, Hauser A, et al. T and B cells participate in bone repair by infiltrating the fracture callus in a two-wave fashion. Bone. 2014;64:155-65 pubmed publisher
Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X, et al. Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity. Clin Vaccine Immunol. 2014;21:156-64 pubmed publisher
Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
Chang J, Cha H, Lee D, Seo K, Kweon M. 1,25-Dihydroxyvitamin D3 inhibits the differentiation and migration of T(H)17 cells to protect against experimental autoimmune encephalomyelitis. PLoS ONE. 2010;5:e12925 pubmed publisher
Cha H, Chang S, Chang J, Kim J, Yang J, Kim C, et al. Downregulation of Th17 cells in the small intestine by disruption of gut flora in the absence of retinoic acid. J Immunol. 2010;184:6799-806 pubmed publisher
Xie X, Stadnisky M, Coats E, Ahmed Rahim M, Lundgren A, Xu W, et al. MHC class I D(k) expression in hematopoietic and nonhematopoietic cells confers natural killer cell resistance to murine cytomegalovirus. Proc Natl Acad Sci U S A. 2010;107:8754-9 pubmed publisher
Qi Q, Xia M, Hu J, Hicks E, Iyer A, Xiong N, et al. Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production. Blood. 2009;114:564-71 pubmed publisher
Schulteis R, Chu H, Dai X, Chen Y, Edwards B, Haribhai D, et al. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood. 2008;112:4905-14 pubmed publisher
Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol Gastrointest Liver Physiol. 2008;295:G273-84 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Chappaz S, Flueck L, Farr A, Rolink A, Finke D. Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice. Blood. 2007;110:3862-70 pubmed
Perchonock C, Pajerowski A, Nguyen C, Shapiro M, Shapiro V. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007;179:1768-75 pubmed
Perchonock C, Fernando M, Quinn W, Nguyen C, Sun J, Shapiro M, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15 pubmed
de Koning B, van Dieren J, Lindenbergh Kortleve D, Van Der Sluis M, Matsumoto T, Yamaguchi K, et al. Contributions of mucosal immune cells to methotrexate-induced mucositis. Int Immunol. 2006;18:941-9 pubmed
Curtsinger J, Lins D, Johnson C, Mescher M. Signal 3 tolerant CD8 T cells degranulate in response to antigen but lack granzyme B to mediate cytolysis. J Immunol. 2005;175:4392-9 pubmed
product information
Antigen :
CD3epsilon
Apps. Abbrev. :
FC
Cat # :
100325
Clone :
145-2C11
Item :
PerCP anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
25 μg
Price (USD) :
88 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PERCP
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis^1, as well as T cell activation assays, inducing proliferation and cytokine production^1,2,7,12,16. It also induces apoptosis in immature thymocytes^32, and in vivo T cell depletion^8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^1, immunohistochemical staining^14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting^4, complement-mediated cytotoxicity^6, in vitro and in vivo stimulation of T cells^1,2,7,12,16, immunofluorescent staining^5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells^11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA