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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse CD3ε
catalog :
100319
quantity :
25 μg
price :
88 USD
clonality :
monoclonal
host :
hamsters
conjugate :
PE/Cy7
clone name :
145-2C11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s3a
Cohen J, Edwards T, Liu A, Hirai T, Jones M, Wu J, et al. Cutaneous TRPV1+ Neurons Trigger Protective Innate Type 17 Anticipatory Immunity. Cell. 2019;178:919-932.e14 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3i
Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
  • flow cytometry; mouse; fig s1d
Kirschbaum K, Sonner J, Zeller M, Deumelandt K, Bode J, Sharma R, et al. In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis. Proc Natl Acad Sci U S A. 2016;113:13227-13232 pubmed
  • flow cytometry; mouse; fig 4
Yasuma K, Yasunaga J, Takemoto K, Sugata K, Mitobe Y, Takenouchi N, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT). PLoS Pathog. 2016;12:e1005372 pubmed publisher
  • flow cytometry; mouse; fig 1
Kamimura D, Katsunuma K, Arima Y, Atsumi T, Jiang J, Bando H, et al. KDEL receptor 1 regulates T-cell homeostasis via PP1 that is a key phosphatase for ISR. Nat Commun. 2015;6:7474 pubmed publisher
  • flow cytometry; mouse; fig 2
Liu B, Lee J, Chen C, Hershey G, Wang Y. Collaborative interactions between type 2 innate lymphoid cells and antigen-specific CD4+ Th2 cells exacerbate murine allergic airway diseases with prominent eosinophilia. J Immunol. 2015;194:3583-93 pubmed publisher
Chen L, Zhou C, Chen Q, Shang J, Liu Z, Guo Y, et al. Oncolytic Zika virus promotes intratumoral T cell infiltration and improves immunotherapy efficacy in glioblastoma. Mol Ther Oncolytics. 2022;24:522-534 pubmed publisher
Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, et al. Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. J Extracell Vesicles. 2022;11:e12197 pubmed publisher
Tacconi C, Commerford C, Dieterich L, Schwager S, He Y, Ikenberg K, et al. CD169+ lymph node macrophages have protective functions in mouse breast cancer metastasis. Cell Rep. 2021;35:108993 pubmed publisher
He Y, Fu L, Li Y, Wang W, Gong M, Zhang J, et al. Gut microbial metabolites facilitate anticancer therapy efficacy by modulating cytotoxic CD8+ T cell immunity. Cell Metab. 2021;33:988-1000.e7 pubmed publisher
Xiao Y, Cong M, Li J, He D, Wu Q, Tian P, et al. Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. Cancer Cell. 2021;39:423-437.e7 pubmed publisher
Westfall S, Caracci F, Zhao D, Wu Q, Frolinger T, Simon J, et al. Microbiota metabolites modulate the T helper 17 to regulatory T cell (Th17/Treg) imbalance promoting resilience to stress-induced anxiety- and depressive-like behaviors. Brain Behav Immun. 2021;91:350-368 pubmed publisher
Wang Y, Wang P, Zhang Y, Xu J, Li Z, Li Z, et al. Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation. Immunity. 2020;53:1168-1181.e7 pubmed publisher
Tuttle K, Waugh K, Araya P, Minter R, Orlicky D, Ludwig M, et al. JAK1 Inhibition Blocks Lethal Immune Hypersensitivity in a Mouse Model of Down Syndrome. Cell Rep. 2020;33:108407 pubmed publisher
Hong J, Reynoso G, Andhey P, Swain A, Turner J, Boon A, et al. An Agonistic Anti-CD137 Antibody Disrupts Lymphoid Follicle Structure and T-Cell-Dependent Antibody Responses. Cell Rep Med. 2020;1: pubmed publisher
Cabañero D, Ramírez López A, Drews E, Schmöle A, Otte D, Wawrzczak Bargiela A, et al. Protective role of neuronal and lymphoid cannabinoid CB2 receptors in neuropathic pain. elife. 2020;9: pubmed publisher
Tran N, Trombke J, Rajewsky K, Chu V. Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells. STAR Protoc. 2020;1:100028 pubmed publisher
Wang H, Sobral M, Zhang D, Cartwright A, Li A, Dellacherie M, et al. Metabolic labeling and targeted modulation of dendritic cells. Nat Mater. 2020;19:1244-1252 pubmed publisher
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Ireland L, Luckett T, Schmid M, Mielgo A. Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis. Front Immunol. 2020;11:297 pubmed publisher
Demircioglu F, Wang J, Candido J, Costa A, Casado P, de Luxán Delgado B, et al. Cancer associated fibroblast FAK regulates malignant cell metabolism. Nat Commun. 2020;11:1290 pubmed publisher
Alikhanyan K, Chen Y, Kraut S, Sotillo R. Targeting alveolar macrophages shows better treatment response than deletion of interstitial macrophages in EGFR mutant lung adenocarcinoma. Immun Inflamm Dis. 2020;8:181-187 pubmed publisher
Maniati E, Berlato C, Gopinathan G, Heath O, Kotantaki P, Lakhani A, et al. Mouse Ovarian Cancer Models Recapitulate the Human Tumor Microenvironment and Patient Response to Treatment. Cell Rep. 2020;30:525-540.e7 pubmed publisher
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
Schadt L, Sparano C, Schweiger N, Silina K, Cecconi V, Lucchiari G, et al. Cancer-Cell-Intrinsic cGAS Expression Mediates Tumor Immunogenicity. Cell Rep. 2019;29:1236-1248.e7 pubmed publisher
Chun E, Lavoie S, Fonseca Pereira D, Bae S, Michaud M, Hoveyda H, et al. Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity. Immunity. 2019;51:871-884.e6 pubmed publisher
Tran N, Sommermann T, Graf R, Trombke J, Pempe J, Petsch K, et al. Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells. Cell Rep. 2019;28:3510-3522.e5 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Tan L, Sandrock I, Odak I, Aizenbud Y, Wilharm A, Barros Martins J, et al. Single-Cell Transcriptomics Identifies the Adaptation of Scart1+ Vγ6+ T Cells to Skin Residency as Activated Effector Cells. Cell Rep. 2019;27:3657-3671.e4 pubmed publisher
Walker J, Clark P, Crisp A, Barlow J, Szeto A, Ferreira A, et al. Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow. Immunity. 2019;: pubmed publisher
Kubota S, Tokunaga K, Umezu T, Yokomizo Nakano T, Sun Y, Oshima M, et al. Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm. Nat Commun. 2019;10:1653 pubmed publisher
Huai W, Liu X, Wang C, Zhang Y, Chen X, Chen X, et al. KAT8 selectively inhibits antiviral immunity by acetylating IRF3. J Exp Med. 2019;216:772-785 pubmed publisher
Zhou J, Peng H, Li K, Qu K, Wang B, Wu Y, et al. Liver-Resident NK Cells Control Antiviral Activity of Hepatic T Cells via the PD-1-PD-L1 Axis. Immunity. 2019;50:403-417.e4 pubmed publisher
Yoshida H, Lareau C, Ramirez R, Rose S, Maier B, Wroblewska A, et al. The cis-Regulatory Atlas of the Mouse Immune System. Cell. 2019;176:897-912.e20 pubmed publisher
Schulthess J, Pandey S, Capitani M, Rue Albrecht K, Arnold I, Franchini F, et al. The Short Chain Fatty Acid Butyrate Imprints an Antimicrobial Program in Macrophages. Immunity. 2019;50:432-445.e7 pubmed publisher
Zhu H, Zhang L, Wu Y, Dong B, Guo W, Wang M, et al. T-ALL leukemia stem cell 'stemness' is epigenetically controlled by the master regulator SPI1. elife. 2018;7: pubmed publisher
Li Z, Liu S, Xu J, Zhang X, Han D, Liu J, et al. Adult Connective Tissue-Resident Mast Cells Originate from Late Erythro-Myeloid Progenitors. Immunity. 2018;49:640-653.e5 pubmed publisher
Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
White J, Xiong S, Malvin N, Khoury Hanold W, Heuckeroth R, Stappenbeck T, et al. Intestinal Dysmotility Syndromes following Systemic Infection by Flaviviruses. Cell. 2018;175:1198-1212.e12 pubmed publisher
Halim T, Rana B, Walker J, Kerscher B, Knolle M, Jolin H, et al. Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells. Immunity. 2018;48:1195-1207.e6 pubmed publisher
Tippimanchai D, Nolan K, Poczobutt J, Verzosa G, Li H, Scarborough H, et al. Adenoviral vectors transduce alveolar macrophages in lung cancer models. Oncoimmunology. 2018;7:e1438105 pubmed publisher
Zhao N, Cao J, Xu L, Tang Q, Dobrolecki L, Lv X, et al. Pharmacological targeting of MYC-regulated IRE1/XBP1 pathway suppresses MYC-driven breast cancer. J Clin Invest. 2018;128:1283-1299 pubmed publisher
Emgård J, Kammoun H, García Cassani B, Chesne J, Parigi S, Jacob J, et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity. 2018;48:120-132.e8 pubmed publisher
Oetjen L, Mack M, Feng J, Whelan T, Niu H, Guo C, et al. Sensory Neurons Co-opt Classical Immune Signaling Pathways to Mediate Chronic Itch. Cell. 2017;171:217-228.e13 pubmed publisher
de Riva A, Wallberg M, Ronchi F, Coulson R, Sage A, Thorne L, et al. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment. PLoS ONE. 2017;12:e0181964 pubmed publisher
Williams J, Elvington A, Ivanov S, Kessler S, Luehmann H, Baba O, et al. Thermoneutrality but Not UCP1 Deficiency Suppresses Monocyte Mobilization Into Blood. Circ Res. 2017;121:662-676 pubmed publisher
Galani I, Triantafyllia V, Eleminiadou E, Koltsida O, Stavropoulos A, Manioudaki M, et al. Interferon-? Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness. Immunity. 2017;46:875-890.e6 pubmed publisher
Uematsu T, Fujita T, Nakaoka H, Hara T, Kobayashi N, Murakami Y, et al. Mint3/Apba3 depletion ameliorates severe murine influenza pneumonia and macrophage cytokine production in response to the influenza virus. Sci Rep. 2016;6:37815 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
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Uematsu T, Iizasa E, Kobayashi N, Yoshida H, Hara H. Loss of CARD9-mediated innate activation attenuates severe influenza pneumonia without compromising host viral immunity. Sci Rep. 2015;5:17577 pubmed publisher
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product information
Antigen :
CD3epsilon
Apps. Abbrev. :
Flow Cytometry
Cat # :
100319
Clone :
145-2C11
Item :
PE/Cyanine7 anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
25 μg
Price (USD) :
88 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA