product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse CD3ε
catalog :
100312
quantity :
100 μg
price :
105 USD
clonality :
monoclonal
host :
hamsters
conjugate :
APC
clone name :
145-2C11
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 95
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig 3a, 3d, s8a
Omatsu Y, Aiba S, Maeta T, Higaki K, Aoki K, Watanabe H, et al. Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells. Nat Commun. 2022;13:2654 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s8a
Bielecki P, Riesenfeld S, Hütter J, Torlai Triglia E, Kowalczyk M, Ricardo Gonzalez R, et al. Skin-resident innate lymphoid cells converge on a pathogenic effector state. Nature. 2021;592:128-132 pubmed publisher
  • flow cytometry; mouse; fig s4d
Phan T, Schink L, Mann J, Merk V, Zwicky P, Mundt S, et al. Keratinocytes control skin immune homeostasis through de novo-synthesized glucocorticoids. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a, 4b, s2, s3a
Antony A, Lian Z, Perrard X, Perrard J, Liu H, Cox A, et al. Deficiency of Stat1 in CD11c+ Cells Alters Adipose Tissue Inflammation and Improves Metabolic Dysfunctions in Mice Fed a High-Fat Diet. Diabetes. 2021;70:720-732 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Rombouts M, Cools N, Grootaert M, de Bakker F, Van Brussel I, Wouters A, et al. Long-Term Depletion of Conventional Dendritic Cells Cannot Be Maintained in an Atherosclerotic Zbtb46-DTR Mouse Model. PLoS ONE. 2017;12:e0169608 pubmed publisher
  • flow cytometry; mouse; fig 1
Pelly V, Kannan Y, Coomes S, Entwistle L, Rückerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
  • flow cytometry; human; fig 1
Sewald X, Ladinsky M, Uchil P, Beloor J, Pi R, Herrmann C, et al. Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infection. Science. 2015;350:563-567 pubmed publisher
  • flow cytometry; mouse
Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
Volpedo G, Pacheco Fernández T, Holcomb E, Zhang W, Lypaczewski P, Cox B, et al. Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis. NPJ Vaccines. 2022;7:32 pubmed publisher
Bajana S, Pankow A, Liu K, Michniowska M, Urban J, Chen W, et al. Correlation between circulating innate lymphoid cell precursors and thymic function. iScience. 2022;25:103732 pubmed publisher
Oksenych V, Su D, Daniel J. Acetyltransferases GCN5 and PCAF Are Required for B Lymphocyte Maturation in Mice. Biomolecules. 2021;12: pubmed publisher
Huang X, Ferris S, Kim S, Choudhary M, Belk J, Fan C, et al. Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis. Immunity. 2021;54:1417-1432.e7 pubmed publisher
Altshuler A, Amitai Lange A, Tarazi N, Dey S, Strinkovsky L, Hadad Porat S, et al. Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing. Cell Stem Cell. 2021;28:1248-1261.e8 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Nahrendorf W, Ivens A, Spence P. Inducible mechanisms of disease tolerance provide an alternative strategy of acquired immunity to malaria. elife. 2021;10: pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Nosho S, Tosa I, Ono M, Hara E, Ishibashi K, Mikai A, et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. Int J Mol Sci. 2020;21: pubmed publisher
Montel Hagen A, Sun V, Casero D, Tsai S, Zampieri A, Jackson N, et al. In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells. Cell Rep. 2020;33:108320 pubmed publisher
Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
Gong N, Zhang Y, Teng X, Wang Y, Huo S, Qing G, et al. Proton-driven transformable nanovaccine for cancer immunotherapy. Nat Nanotechnol. 2020;15:1053-1064 pubmed publisher
Arensman M, Yang X, Zhong W, Bisulco S, Upeslacis E, Rosfjord E, et al. Anti-tumor immunity influences cancer cell reliance upon ATG7. Oncoimmunology. 2020;9:1800162 pubmed publisher
McCauley M, O Rourke J, Yáñez A, Markman J, Ho R, Wang X, et al. C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature. 2020;585:96-101 pubmed publisher
Parhi L, Alon Maimon T, Sol A, Nejman D, Shhadeh A, Fainsod Levi T, et al. Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression. Nat Commun. 2020;11:3259 pubmed publisher
Ulaganathan V. TraPS-VarI: Identifying genetic variants altering phosphotyrosine based signalling motifs. Sci Rep. 2020;10:8453 pubmed publisher
Ferrari de Andrade L, Kumar S, Luoma A, Ito Y, Alves da Silva P, Pan D, et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res. 2020;8:769-780 pubmed publisher
Wang Q, Pujol Canadell M, Taveras M, Garty G, Perrier J, Bueno Betí C, et al. DNA damage response in peripheral mouse blood leukocytes in vivo after variable, low-dose rate exposure. Radiat Environ Biophys. 2020;59:89-98 pubmed publisher
Ghandhi S, Smilenov L, Shuryak I, Pujol Canadell M, Amundson S. Discordant gene responses to radiation in humans and mice and the role of hematopoietically humanized mice in the search for radiation biomarkers. Sci Rep. 2019;9:19434 pubmed publisher
Castañeda Zegarra S, Huse C, Røsand Ø, Sarno A, Xing M, Gago Fuentes R, et al. Generation of a Mouse Model Lacking the Non-Homologous End-Joining Factor Mri/Cyren. Biomolecules. 2019;9: pubmed publisher
Lercher A, Bhattacharya A, Popa A, Caldera M, Schlapansky M, Baazim H, et al. Type I Interferon Signaling Disrupts the Hepatic Urea Cycle and Alters Systemic Metabolism to Suppress T Cell Function. Immunity. 2019;51:1074-1087.e9 pubmed publisher
Gao J, Owusu Ansah A, Paun A, Beacht K, Yim E, Siwicki M, et al. Low-level Cxcr4-haploinsufficient HSC engraftment is sufficient to correct leukopenia in WHIM syndrome mice. JCI Insight. 2019;4: pubmed publisher
Xu H, Ding J, Porter C, Wallrapp A, Tabaka M, Ma S, et al. Transcriptional Atlas of Intestinal Immune Cells Reveals that Neuropeptide α-CGRP Modulates Group 2 Innate Lymphoid Cell Responses. Immunity. 2019;51:696-708.e9 pubmed publisher
Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Chan L, Li C, Xia W, Hsu J, Lee H, Cha J, et al. IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasion. J Clin Invest. 2019;129:3324-3338 pubmed publisher
Fujita K, Chakarov S, Kobayashi T, Sakamoto K, Voisin B, Duan K, et al. Cell-autonomous FLT3L shedding via ADAM10 mediates conventional dendritic cell development in mouse spleen. Proc Natl Acad Sci U S A. 2019;116:14714-14723 pubmed publisher
Omenetti S, Bussi C, Metidji A, Iseppon A, Lee S, Tolaini M, et al. The Intestine Harbors Functionally Distinct Homeostatic Tissue-Resident and Inflammatory Th17 Cells. Immunity. 2019;: pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Radulović V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, et al. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis. Cell Rep. 2019;27:2826-2836.e5 pubmed publisher
Ajina R, Zamalin D, Zuo A, Moussa M, Catalfamo M, Jablonski S, et al. SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent mice. Oncoimmunology. 2019;8:e1577127 pubmed publisher
Agelidis A, Koujah L, Suryawanshi R, Yadavalli T, Mishra Y, Adelung R, et al. An Intra-Vaginal Zinc Oxide Tetrapod Nanoparticles (ZOTEN) and Genital Herpesvirus Cocktail Can Provide a Novel Platform for Live Virus Vaccine. Front Immunol. 2019;10:500 pubmed publisher
Codina A, Renauer P, Wang G, Chow R, Park J, Ye H, et al. Convergent Identification and Interrogation of Tumor-Intrinsic Factors that Modulate Cancer Immunity In Vivo. Cell Syst. 2019;8:136-151.e7 pubmed publisher
Kang Y, Biswas A, Field M, Snapper S. STAT1 signaling shields T cells from NK cell-mediated cytotoxicity. Nat Commun. 2019;10:912 pubmed publisher
Parks C, Henning K, Pavelko K, Hansen M, Van Keulen V, Reed B, et al. Breaking tolerance with engineered class I antigen-presenting molecules. Proc Natl Acad Sci U S A. 2019;116:3136-3145 pubmed publisher
Tong Y, Zhou L, Yang L, Guo P, Cao Y, Qin F, et al. Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production. EBioMedicine. 2019;39:132-144 pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Gubin M, Esaulova E, Ward J, Malkova O, Runci D, Wong P, et al. High-Dimensional Analysis Delineates Myeloid and Lymphoid Compartment Remodeling during Successful Immune-Checkpoint Cancer Therapy. Cell. 2018;175:1014-1030.e19 pubmed publisher
Casanova Acebes M, Nicolás Ávila J, Li J, Garcia Silva S, Balachander A, Rubio Ponce A, et al. Neutrophils instruct homeostatic and pathological states in naive tissues. J Exp Med. 2018;215:2778-2795 pubmed publisher
Metidji A, Omenetti S, Crotta S, Li Y, Nye E, Ross E, et al. The Environmental Sensor AHR Protects from Inflammatory Damage by Maintaining Intestinal Stem Cell Homeostasis and Barrier Integrity. Immunity. 2018;49:353-362.e5 pubmed publisher
Liu S, Xia Q, Wu X, Sun F, Hu Q, Wu J, et al. Stimulator of Interferon Genes in Classical Dendritic Cells Controls Mucosal Th17 Responses to Cyclic Dinucleotides for Host Defenses Against Microbial Infections in Gut. Front Immunol. 2018;9:1085 pubmed publisher
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, et al. A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018;48:688-701.e7 pubmed publisher
Wang J, Liu S, Hou B, Yang M, Dong Z, Qi H, et al. PTEN-Regulated AID Transcription in Germinal Center B Cells Is Essential for the Class-Switch Recombination and IgG Antibody Responses. Front Immunol. 2018;9:371 pubmed publisher
Glasner A, Isaacson B, Viukov S, Neuman T, Friedman N, Mandelboim M, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression. Sci Rep. 2017;7:13090 pubmed publisher
Harvey R, Howard V, Lemus M, Jois T, Andrews Z, Sleeman M. The Ghrelin/GOAT System Regulates Obesity-Induced Inflammation in Male Mice. Endocrinology. 2017;158:2179-2189 pubmed publisher
Miura N, Akita H, Tateshita N, Nakamura T, Harashima H. Modifying Antigen-Encapsulating Liposomes with KALA Facilitates MHC Class I Antigen Presentation and Enhances Anti-tumor Effects. Mol Ther. 2017;25:1003-1013 pubmed publisher
Gorczynski R, Sadozai H, Zhu F, Khatri I. Effect of infusion of monoclonal antibodies to tumour necrosis factor-receptor super family 25 on graft rejection in allo-immune mice receiving autologous marrow transplantation. Immunology. 2017;150:418-431 pubmed publisher
Roufaiel M, Gracey E, Siu A, Zhu S, Lau A, Ibrahim H, et al. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima. Nat Immunol. 2016;17:1263-1272 pubmed publisher
Wang Y, Jiang X, Zhu J, Dan Yue -, Zhang X, Wang X, et al. IL-21/IL-21R signaling suppresses intestinal inflammation induced by DSS through regulation of Th responses in lamina propria in mice. Sci Rep. 2016;6:31881 pubmed publisher
Campisi L, Barbet G, Ding Y, Esplugues E, Flavell R, Blander J. Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol. 2016;17:1084-92 pubmed publisher
Barrett N, Malouf C, Kapeni C, Bacon W, Giotopoulos G, Jacobsen S, et al. Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development. Cell Rep. 2016;16:1039-1054 pubmed publisher
Ku M, Edes I, Bendix I, Pohlmann A, Waiczies H, Prozorovski T, et al. ERK1 as a Therapeutic Target for Dendritic Cell Vaccination against High-Grade Gliomas. Mol Cancer Ther. 2016;15:1975-87 pubmed publisher
Uto T, Fukaya T, Takagi H, Arimura K, Nakamura T, Kojima N, et al. Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity. Nat Commun. 2016;7:11273 pubmed publisher
Yu Y, O Koren E, Hotten D, Kan M, Kopin D, Nelson E, et al. A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues. PLoS ONE. 2016;11:e0150606 pubmed publisher
Cruz F, Borg Z, Goodwin M, Coffey A, Wagner D, Rocco P, et al. CD11b+ and Sca-1+ Cells Exert the Main Beneficial Effects of Systemically Administered Bone Marrow-Derived Mononuclear Cells in a Murine Model of Mixed Th2/Th17 Allergic Airway Inflammation. Stem Cells Transl Med. 2016;5:488-99 pubmed publisher
Leiva M, Quintana J, Ligos J, Hidalgo A. Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability. Nat Commun. 2016;7:10222 pubmed publisher
Holvoet B, Quattrocelli M, Belderbos S, Pollaris L, Wolfs E, Gheysens O, et al. Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice. Stem Cell Reports. 2015;5:1183-1195 pubmed publisher
Ito C, Okuyama Dobashi K, Miyasaka T, Masuda C, Sato M, Kawano T, et al. CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma. PLoS ONE. 2015;10:e0140808 pubmed publisher
Siggs O, Popkin D, Krebs P, Li X, Tang M, Zhan X, et al. Mutation of the ER retention receptor KDELR1 leads to cell-intrinsic lymphopenia and a failure to control chronic viral infection. Proc Natl Acad Sci U S A. 2015;112:E5706-14 pubmed publisher
Burrack K, Tan J, McCarthy M, Her Z, Berger J, Ng L, et al. Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells. PLoS Pathog. 2015;11:e1005191 pubmed publisher
Sato K, Yamamoto H, Nomura T, Matsumoto I, Miyasaka T, Zong T, et al. Cryptococcus neoformans Infection in Mice Lacking Type I Interferon Signaling Leads to Increased Fungal Clearance and IL-4-Dependent Mucin Production in the Lungs. PLoS ONE. 2015;10:e0138291 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Tournier C, Reina M, Espel E. Dual role of ERK5 in the regulation of T cell receptor expression at the T cell surface. J Leukoc Biol. 2016;99:143-52 pubmed publisher
Xu G, Wu H, Zhang J, Li D, Wang Y, Wang Y, et al. Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free Radic Biol Med. 2015;87:15-25 pubmed publisher
Luck H, Tsai S, Chung J, Clemente Casares X, Ghazarian M, Revelo X, et al. Regulation of obesity-related insulin resistance with gut anti-inflammatory agents. Cell Metab. 2015;21:527-42 pubmed publisher
Burrack K, Montgomery S, Homann D, Morrison T. CD8+ T cells control Ross River virus infection in musculoskeletal tissues of infected mice. J Immunol. 2015;194:678-89 pubmed publisher
Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, et al. A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice. J Immunol. 2015;194:243-51 pubmed publisher
Pennock N, Gapin L, Kedl R. IL-27 is required for shaping the magnitude, affinity distribution, and memory of T cells responding to subunit immunization. Proc Natl Acad Sci U S A. 2014;111:16472-7 pubmed publisher
Revelo X, Tsai S, Lei H, Luck H, Ghazarian M, Tsui H, et al. Perforin is a novel immune regulator of obesity-related insulin resistance. Diabetes. 2015;64:90-103 pubmed publisher
Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
Aebischer D, Willrodt A, Halin C. Oxazolone-induced contact hypersensitivity reduces lymphatic drainage but enhances the induction of adaptive immunity. PLoS ONE. 2014;9:e99297 pubmed publisher
Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, et al. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting ROR?t and STAT3. J Immunol. 2014;192:5599-609 pubmed publisher
Bergquist M, Jirholt P, Nurkkala M, Rylander C, Hedenstierna G, Lindholm C. Glucocorticoid receptor function is decreased in neutrophils during endotoxic shock. J Infect. 2014;69:113-22 pubmed publisher
Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
Okamoto M, Oshiumi H, Azuma M, Kato N, Matsumoto M, Seya T. IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection. J Immunol. 2014;192:2770-7 pubmed publisher
Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, et al. α-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3. J Immunol. 2014;192:1972-81 pubmed publisher
Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X, et al. Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity. Clin Vaccine Immunol. 2014;21:156-64 pubmed publisher
Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
Qi Q, Xia M, Hu J, Hicks E, Iyer A, Xiong N, et al. Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production. Blood. 2009;114:564-71 pubmed publisher
Schulteis R, Chu H, Dai X, Chen Y, Edwards B, Haribhai D, et al. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood. 2008;112:4905-14 pubmed publisher
Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol Gastrointest Liver Physiol. 2008;295:G273-84 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Chappaz S, Flueck L, Farr A, Rolink A, Finke D. Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice. Blood. 2007;110:3862-70 pubmed
Perchonock C, Pajerowski A, Nguyen C, Shapiro M, Shapiro V. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007;179:1768-75 pubmed
Perchonock C, Fernando M, Quinn W, Nguyen C, Sun J, Shapiro M, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15 pubmed
de Koning B, van Dieren J, Lindenbergh Kortleve D, Van Der Sluis M, Matsumoto T, Yamaguchi K, et al. Contributions of mucosal immune cells to methotrexate-induced mucositis. Int Immunol. 2006;18:941-9 pubmed
Curtsinger J, Lins D, Johnson C, Mescher M. Signal 3 tolerant CD8 T cells degranulate in response to antigen but lack granzyme B to mediate cytolysis. J Immunol. 2005;175:4392-9 pubmed
product information
Antigen :
CD3epsilon
Apps. Abbrev. :
Flow Cytometry
Cat # :
100312
Clone :
145-2C11
Item :
APC anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
100 μg
Price (USD) :
105 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
APC
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA