PE/Cyanine5 anti-mouse CD3ε | BioLegend 100309 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-mouse CD3ε

catalog :

100309

quantity :

25 μg

price :

70 USD

clonality :

monoclonal

host :

hamsters

conjugate :

PE-Cy5

clone name :

145-2C11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s2f	Mathew N, Baumgartner F, Braun L, O Sullivan D, Thomas S, Waterhouse M, et al. Sorafenib promotes graft-versus-leukemia activity in mice and humans through IL-15 production in FLT3-ITD-mutant leukemia cells. Nat Med. 2018;24:282-291 pubmed publisher
	Ciecko A, Foda B, Barr J, Ramanathan S, Atkinson M, Serreze D, et al. Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation. Cell Rep. 2019;29:3073-3086.e5 pubmed publisher
	Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
	Radulović V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, et al. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis. Cell Rep. 2019;27:2826-2836.e5 pubmed publisher
	Di Genua C, Norfo R, Rodriguez Meira A, Wen W, Drissen R, Booth C, et al. Cell-intrinsic depletion of Aml1-ETO-expressing pre-leukemic hematopoietic stem cells by K-Ras activating mutation. Haematologica. 2019;: pubmed publisher
	Halvarsson C, Rörby E, Eliasson P, Lang S, Soneji S, Jönsson J. Putative role of NF-kB but not HIF-1α in hypoxia-dependent regulation of oxidative stress in hematopoietic stem and progenitor cells. Antioxid Redox Signal. 2019;: pubmed publisher
	Säwén P, Eldeeb M, Erlandsson E, Kristiansen T, Laterza C, Kokaia Z, et al. Murine HSCs contribute actively to native hematopoiesis but with reduced differentiation capacity upon aging. elife. 2018;7: pubmed publisher
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	Marcus A, Mao A, Lensink Vasan M, Wang L, Vance R, Raulet D. Tumor-Derived cGAMP Triggers a STING-Mediated Interferon Response in Non-tumor Cells to Activate the NK Cell Response. Immunity. 2018;49:754-763.e4 pubmed publisher
	Peltzer N, Darding M, Montinaro A, Dráber P, Draberova H, Kupka S, et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature. 2018;557:112-117 pubmed publisher
	Zhou T, Zhu M, Liang Z. (-)-Epigallocatechin-3-gallate modulates peripheral immunity in the MPTP-induced mouse model of Parkinson's disease. Mol Med Rep. 2018;17:4883-4888 pubmed publisher
	Natale C, Li J, Zhang J, Dahal A, Dentchev T, Stanger B, et al. Activation of G protein-coupled estrogen receptor signaling inhibits melanoma and improves response to immune checkpoint blockade. elife. 2018;7: pubmed publisher
	Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
	Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, et al. Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth. elife. 2017;6: pubmed publisher
	Naik A, Hawwari A, Krangel M. Specification of VÎ´ and VÎ± usage by Tcra/Tcrd locus V gene segment promoters. J Immunol. 2015;194:790-4 pubmed publisher
	Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
	Koma T, Yoshimatsu K, Nagata N, Sato Y, Shimizu K, Yasuda S, et al. Neutrophil depletion suppresses pulmonary vascular hyperpermeability and occurrence of pulmonary edema caused by hantavirus infection in C.B-17 SCID mice. J Virol. 2014;88:7178-88 pubmed publisher
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	Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
	Balkow S, Heinz S, Schmidbauer P, Kolanus W, Holzmann B, Grabbe S, et al. LFA-1 activity state on dendritic cells regulates contact duration with T cells and promotes T-cell priming. Blood. 2010;116:1885-94 pubmed publisher
	Li X, Liu Y, Knight D, Odaka Y, Mathis J, Shi R, et al. Effect of B7.1 costimulation on T-cell based immunity against TAP-negative cancer can be facilitated by TAP1 expression. PLoS ONE. 2009;4:e6385 pubmed publisher
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product information

Antigen :

CD3epsilon

Apps. Abbrev. :

Flow Cytometry

Cat # :

100309

Clone :

145-2C11

Item :

PE/Cyanine5 anti-mouse CD3ε

Isotype :

Armenian Hamster IgG

Other Names :

CD3ε, T3, CD3

Size :

25 μg

Price (USD) :

70 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

PE_CYANINE5

Immunogen :

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Application Notes :

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).

more info or order :

BioLegend product webpage