product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD3ε
catalog :
100308
quantity :
200 μg
price :
114 USD
clonality :
monoclonal
host :
hamsters
conjugate :
PE
clone name :
145-2C11
reactivity :
mouse
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 79
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 5b
Melese E, Franks E, Cederberg R, Harbourne B, Shi R, Wadsworth B, et al. CCL5 production in lung cancer cells leads to an altered immune microenvironment and promotes tumor development. Oncoimmunology. 2022;11:2010905 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 4a, s3a
Wutschka J, Kast B, Sator Schmitt M, Appak Baskoy S, Hess J, Sinn H, et al. JUNB suppresses distant metastasis by influencing the initial metastatic stage. Clin Exp Metastasis. 2021;38:411-423 pubmed publisher
  • flow cytometry; mouse; loading ...
Gvozdeva O, Achasova K, Litvinova N, Kozhevnikova E, Litvinova E. Female Scent Activated Expression of Arginase1 and Inducible NO-Synthetase in Lung of BALB/c Male Mice. Animals (Basel). 2021;11: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s8a
Bielecki P, Riesenfeld S, Hütter J, Torlai Triglia E, Kowalczyk M, Ricardo Gonzalez R, et al. Skin-resident innate lymphoid cells converge on a pathogenic effector state. Nature. 2021;592:128-132 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1a, s2g
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s4d
Tang H, Liang Y, Anders R, Taube J, Qiu X, Mulgaonkar A, et al. PD-L1 on host cells is essential for PD-L1 blockade-mediated tumor regression. J Clin Invest. 2018;128:580-588 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Cecchinato V, Bernasconi E, Speck R, Proietti M, Sauermann U, D Agostino G, et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol. 2017;198:184-195 pubmed
  • flow cytometry; mouse; fig 1b
Takeda Y, Azuma M, Matsumoto M, Seya T. Tumoricidal efficacy coincides with CD11c up-regulation in antigen-specific CD8(+) T cells during vaccine immunotherapy. J Exp Clin Cancer Res. 2016;35:143 pubmed publisher
  • immunocytochemistry; mouse; 1:800
Boulay A, Mazeraud A, Cisternino S, Saubaméa B, Mailly P, Jourdren L, et al. Immune quiescence of the brain is set by astroglial connexin 43. J Neurosci. 2015;35:4427-39 pubmed publisher
  • flow cytometry; mouse; fig 1
Salinas N, Olguín J, Castellanos C, Saavedra R. T cell suppression in vitro during Toxoplasma gondii infection is the result of IL-2 competition between Tregs and T cells leading to death of proliferating T cells. Scand J Immunol. 2014;79:1-11 pubmed publisher
Abdelfattah N, Kumar P, Wang C, Leu J, Flynn W, Gao R, et al. Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. Nat Commun. 2022;13:767 pubmed publisher
Hara T, Chanoch Myers R, Mathewson N, Myskiw C, Atta L, Bussema L, et al. Interactions between cancer cells and immune cells drive transitions to mesenchymal-like states in glioblastoma. Cancer Cell. 2021;39:779-792.e11 pubmed publisher
Mah Som A, Keppel M, Tobin J, Kolicheski A, Saucier N, Sexl V, et al. Reliance on Cox10 and oxidative metabolism for antigen-specific NK cell expansion. Cell Rep. 2021;35:109209 pubmed publisher
Avgustinova A, Laudanna C, Pascual García M, Rovira Q, Djurec M, Castellanos A, et al. Repression of endogenous retroviruses prevents antiviral immune response and is required for mammary gland development. Cell Stem Cell. 2021;: pubmed publisher
Chulpanova D, Gilazieva Z, Kletukhina S, Aimaletdinov A, Garanina E, James V, et al. Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer Cells. Biology (Basel). 2021;10: pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Faust H, Zhang H, Han J, Wolf M, Jeon O, Sadtler K, et al. IL-17 and immunologically induced senescence regulate response to injury in osteoarthritis. J Clin Invest. 2020;130:5493-5507 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Hu K, Eichorst J, McGinnis C, Patterson D, Chow E, Kersten K, et al. ZipSeq: barcoding for real-time mapping of single cell transcriptomes. Nat Methods. 2020;17:833-843 pubmed publisher
Gomzikova M, Kletukhina S, Kurbangaleeva S, Neustroeva O, Vasileva O, Garanina E, et al. Mesenchymal Stem Cell Derived Biocompatible Membrane Vesicles Demonstrate Immunomodulatory Activity Inhibiting Activation and proliferation of Human Mononuclear Cells. Pharmaceutics. 2020;12: pubmed publisher
Siamishi I, Iwanami N, Clapes T, Trompouki E, O Meara C, Boehm T. Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020;31:107756 pubmed publisher
Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Rep. 2020;31:107628 pubmed publisher
Sarapulov A, Petrov P, Hernández Pérez S, Sustar V, Kuokkanen E, Cords L, et al. Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses. Front Immunol. 2020;11:599 pubmed publisher
Chang Y, Ko B, Ju J, Chang H, Huang S, Lin C. SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation. Int J Mol Sci. 2020;21: pubmed publisher
Grødeland G, Baranowska Hustad M, Abadejos J, Blane T, Teijaro J, Nemazee D, et al. Induction of Cross-Reactive and Protective Antibody Responses After DNA Vaccination With MHCII-Targeted Stem Domain From Influenza Hemagglutinin. Front Immunol. 2020;11:431 pubmed publisher
Yu X, Dai Y, Zhao Y, Qi S, Liu L, Lu L, et al. Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response. Nat Commun. 2020;11:1110 pubmed publisher
Kristensen L, Christensen C, Alfsen M, Cold S, Nielsen C, Kjaer A. Monitoring CD8a+ T Cell Responses to Radiotherapy and CTLA-4 Blockade Using [64Cu]NOTA-CD8a PET Imaging. Mol Imaging Biol. 2020;22:1021-1030 pubmed publisher
Qi S, Lu L, Zhou F, Chen Y, Xu M, Chen L, et al. Neutrophil infiltration and whole-cell vaccine elicited by N-dihydrogalactochitosan combined with NIR phototherapy to enhance antitumor immune response and T cell immune memory. Theranostics. 2020;10:1814-1832 pubmed publisher
Kretschmer L, Flossdorf M, Mir J, Cho Y, Plambeck M, Treise I, et al. Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nat Commun. 2020;11:113 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Miller B, Yates K, Gillis J, et al. PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019;20:1335-1347 pubmed publisher
He L, Zhou M, Li Y. Vitamin D/Vitamin D Receptor Signaling Is Required for Normal Development and Function of Group 3 Innate Lymphoid Cells in the Gut. iScience. 2019;17:119-131 pubmed publisher
Kubli S, Vornholz L, Duncan G, Zhou W, Ramachandran P, Fortin J, et al. Fcmr regulates mononuclear phagocyte control of anti-tumor immunity. Nat Commun. 2019;10:2678 pubmed publisher
Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
Leal A, Misek S, Lisabeth E, Neubig R, Liby K. The Rho/MRTF pathway inhibitor CCG-222740 reduces stellate cell activation and modulates immune cell populations in KrasG12D; Pdx1-Cre (KC) mice. Sci Rep. 2019;9:7072 pubmed publisher
Ji X, Hao J, Li G, Dong N, Wang X, Zhou M, et al. Exendin-4 Exacerbates Burn-Induced Morbidity in Mice by Activation of the Sympathetic Nervous System. Mediators Inflamm. 2019;2019:2750528 pubmed publisher
Dyer D, Medina Ruiz L, Bartolini R, Schuette F, Hughes C, Pallas K, et al. Chemokine Receptor Redundancy and Specificity Are Context Dependent. Immunity. 2019;50:378-389.e5 pubmed publisher
Jayachandran R, Gumienny A, Bolinger B, Ruehl S, Lang M, Fucile G, et al. Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity. 2019;50:152-165.e8 pubmed publisher
Hiraishi Y, Yamaguchi S, Yoshizaki T, Nambu A, Shimura E, Takamori A, et al. IL-33, IL-25 and TSLP contribute to development of fungal-associated protease-induced innate-type airway inflammation. Sci Rep. 2018;8:18052 pubmed publisher
Malik A, Sharma D, Malireddi R, Guy C, Chang T, Olsen S, et al. SYK-CARD9 Signaling Axis Promotes Gut Fungi-Mediated Inflammasome Activation to Restrict Colitis and Colon Cancer. Immunity. 2018;49:515-530.e5 pubmed publisher
Smith L, Boukhaled G, Condotta S, Mazouz S, Guthmiller J, Vijay R, et al. Interleukin-10 Directly Inhibits CD8+ T Cell Function by Enhancing N-Glycan Branching to Decrease Antigen Sensitivity. Immunity. 2018;48:299-312.e5 pubmed publisher
Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze L, et al. Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction. Immunity. 2017;47:1114-1128.e6 pubmed publisher
Sierro F, Evrard M, Rizzetto S, Melino M, Mitchell A, Flórido M, et al. A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment. Immunity. 2017;47:374-388.e6 pubmed publisher
Pan W, Zhu S, Qu K, Meeth K, Cheng J, He K, et al. The DNA Methylcytosine Dioxygenase Tet2 Sustains Immunosuppressive Function of Tumor-Infiltrating Myeloid Cells to Promote Melanoma Progression. Immunity. 2017;47:284-297.e5 pubmed publisher
Dave K, Sur I, Yan J, Zhang J, Kaasinen E, Zhong F, et al. Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth. elife. 2017;6: pubmed publisher
Celià Terrassa T, Liu D, Choudhury A, Hang X, Wei Y, Zamalloa J, et al. Normal and cancerous mammary stem cells evade interferon-induced constraint through the miR-199a-LCOR axis. Nat Cell Biol. 2017;19:711-723 pubmed publisher
Thelin M, Kissler S, Vigneault F, Watters A, White D, Koshy S, et al. In Vivo Enrichment of Diabetogenic T Cells. Diabetes. 2017;66:2220-2229 pubmed publisher
Bansal K, Yoshida H, Benoist C, Mathis D. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol. 2017;18:263-273 pubmed publisher
Qi S, Li H, Lu L, Qi Z, Liu L, Chen L, et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. elife. 2016;5: pubmed publisher
Kim D, Lee S, Jun H. Impact of T-cell-specific Smad4 deficiency on the development of autoimmune diabetes in NOD mice. Immunol Cell Biol. 2017;95:287-296 pubmed publisher
Zhang B, Kwon O, Henry G, Malewska A, Wei X, Zhang L, et al. Non-Cell-Autonomous Regulation of Prostate Epithelial Homeostasis by Androgen Receptor. Mol Cell. 2016;63:976-89 pubmed publisher
Qiu Z, Cervantes J, Cicek B, Mukherjee S, Venkatesh M, Maher L, et al. Pregnane X Receptor Regulates Pathogen-Induced Inflammation and Host Defense against an Intracellular Bacterial Infection through Toll-like Receptor 4. Sci Rep. 2016;6:31936 pubmed publisher
Campisi L, Barbet G, Ding Y, Esplugues E, Flavell R, Blander J. Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol. 2016;17:1084-92 pubmed publisher
Guo H, Cooper S, Friedman A. In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS ONE. 2016;11:e0150809 pubmed publisher
Kim I, Mlsna L, Yoon S, Le B, Yu S, Xu D, et al. A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers. Brain Behav. 2015;5:e00403 pubmed publisher
Ma J, Kim J, Kazane S, Choi S, Yun H, Kim M, et al. Versatile strategy for controlling the specificity and activity of engineered T cells. Proc Natl Acad Sci U S A. 2016;113:E450-8 pubmed publisher
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product information
Antigen :
CD3epsilon
Apps. Abbrev. :
Flow Cytometry
Cat # :
100308
Clone :
145-2C11
Item :
PE anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
200 μg
Price (USD) :
114 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PE
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA