product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse CD3ε
catalog :
100304
quantity :
500 μg
price :
110 USD
clonality :
monoclonal
host :
hamsters
conjugate :
biotin
clone name :
145-2C11
reactivity :
mouse
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s2a
Tang A, Choi J, Kotzin J, Yang Y, Hong C, Hobson N, et al. Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature. 2017;545:305-310 pubmed publisher
  • blocking or activating experiments; mouse; 10 ug/ml
Okada T, Nitta T, Kaji K, Takashima A, Oda H, Tamehiro N, et al. Differential function of Themis CABIT domains during T cell development. PLoS ONE. 2014;9:e89115 pubmed publisher
Domingues A, Kulkarni R, Giotopoulos G, Gupta S, Vinnenberg L, Arede L, et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. elife. 2020;9: pubmed publisher
O Boyle C, Haley M, Lemarchand E, Smith C, Allan S, Konkel J, et al. Ligature-induced periodontitis induces systemic inflammation but does not alter acute outcome after stroke in mice. Int J Stroke. 2020;15:175-187 pubmed publisher
Hurrell B, Galle Treger L, Jahani P, Howard E, Helou D, Banie H, et al. TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity. Cell Rep. 2019;29:4509-4524.e5 pubmed publisher
van der Poel C, Bajic G, Macaulay C, van den Broek T, Ellson C, Bouma G, et al. Follicular Dendritic Cells Modulate Germinal Center B Cell Diversity through FcγRIIB. Cell Rep. 2019;29:2745-2755.e4 pubmed publisher
Onodera T, Hashi K, Shukla R, Miki M, Takai Todaka R, Fujimoto A, et al. Immune-Focusing Properties of Virus-like Particles Improve Protective IgA Responses. J Immunol. 2019;203:3282-3292 pubmed publisher
Davis F, Schaller M, denDekker A, Joshi A, Kimball A, Evanoff H, et al. Sepsis Induces Prolonged Epigenetic Modifications in Bone Marrow and Peripheral Macrophages Impairing Inflammation and Wound Healing. Arterioscler Thromb Vasc Biol. 2019;39:2353-2366 pubmed publisher
Adachi Y, Tonouchi K, Nithichanon A, Kuraoka M, Watanabe A, Shinnakasu R, et al. Exposure of an occluded hemagglutinin epitope drives selection of a class of cross-protective influenza antibodies. Nat Commun. 2019;10:3883 pubmed publisher
Roco J, Mesin L, Binder S, Nefzger C, Gonzalez Figueroa P, Canete P, et al. Class-Switch Recombination Occurs Infrequently in Germinal Centers. Immunity. 2019;51:337-350.e7 pubmed publisher
Nagashima H, Mahlakõiv T, Shih H, Davis F, Meylan F, Huang Y, et al. Neuropeptide CGRP Limits Group 2 Innate Lymphoid Cell Responses and Constrains Type 2 Inflammation. Immunity. 2019;51:682-695.e6 pubmed publisher
Kimball A, Davis F, denDekker A, Joshi A, Schaller M, Bermick J, et al. The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair. Immunity. 2019;51:258-271.e5 pubmed publisher
Park D, Akuffo A, Muench D, Grimes H, Epling Burnette P, Maini P, et al. Clonal hematopoiesis of indeterminate potential and its impact on patient trajectories after stem cell transplantation. PLoS Comput Biol. 2019;15:e1006913 pubmed publisher
Goldstein J, Tabebordbar M, Zhu K, Wang L, Messemer K, Peacker B, et al. In Situ Modification of Tissue Stem and Progenitor Cell Genomes. Cell Rep. 2019;27:1254-1264.e7 pubmed publisher
Matsumura T, Ikebe T, Arikawa K, Hosokawa M, Aiko M, Iguchi A, et al. Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep. 2019;27:561-571.e6 pubmed publisher
Balzano M, De Grandis M, Vu Manh T, Chasson L, Bardin F, Farina A, et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep. 2019;26:3257-3271.e8 pubmed publisher
Galle Treger L, Sankaranarayanan I, Hurrell B, Howard E, Lo R, Maazi H, et al. Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes. Nat Commun. 2019;10:713 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Mann M, Mehta A, de Boer C, Kowalczyk M, Lee K, Haldeman P, et al. Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with Age. Cell Rep. 2018;25:2992-3005.e5 pubmed publisher
Giambra V, Gusscott S, Gracias D, Song R, Lam S, Panelli P, et al. Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity. Cell Stem Cell. 2018;23:714-726.e7 pubmed publisher
Tanimura N, Liao R, Wilson G, Dent M, Cao M, Burstyn J, et al. GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism. Dev Cell. 2018;46:581-594.e4 pubmed publisher
Kimball A, Schaller M, Joshi A, Davis F, denDekker A, Boniakowski A, et al. Ly6CHi Blood Monocyte/Macrophage Drive Chronic Inflammation and Impair Wound Healing in Diabetes Mellitus. Arterioscler Thromb Vasc Biol. 2018;38:1102-1114 pubmed publisher
Johnson J, Georgakilas G, Petrovic J, Kurachi M, Cai S, Harly C, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells. Immunity. 2018;48:243-257.e10 pubmed publisher
Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
Smith L, Boukhaled G, Condotta S, Mazouz S, Guthmiller J, Vijay R, et al. Interleukin-10 Directly Inhibits CD8+ T Cell Function by Enhancing N-Glycan Branching to Decrease Antigen Sensitivity. Immunity. 2018;48:299-312.e5 pubmed publisher
de Mingo Pulido Á, Gardner A, Hiebler S, Soliman H, Rugo H, Krummel M, et al. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell. 2018;33:60-74.e6 pubmed publisher
Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
Kim O, Park H, Dinh N, Choi S, Lee J, Kim J, et al. Bacterial outer membrane vesicles suppress tumor by interferon-?-mediated antitumor response. Nat Commun. 2017;8:626 pubmed publisher
Bieber K, Witte M, Sun S, Hundt J, Kalies K, Dräger S, et al. T cells mediate autoantibody-induced cutaneous inflammation and blistering in epidermolysis bullosa acquisita. Sci Rep. 2016;6:38357 pubmed publisher
Zysset D, Weber B, Rihs S, Brasseit J, Freigang S, Riether C, et al. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nat Commun. 2016;7:13151 pubmed publisher
Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, et al. Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. Nature. 2016;537:698-702 pubmed publisher
Kurkewich J, Klopfenstein N, Hallas W, Wood C, Sattler R, Das C, et al. Arid3b Is Critical for B Lymphocyte Development. PLoS ONE. 2016;11:e0161468 pubmed publisher
Lim H, Cordoba S, Dushek O, Goyette J, Taylor A, Rudd C, et al. Costimulation of IL-2 Production through CD28 Is Dependent on the Size of Its Ligand. J Immunol. 2015;195:5432-9 pubmed publisher
Doisne J, Balmas E, Boulenouar S, Gaynor L, Kieckbusch J, Gardner L, et al. Composition, Development, and Function of Uterine Innate Lymphoid Cells. J Immunol. 2015;195:3937-45 pubmed publisher
Pearce V, Bouabe H, MacQueen A, Carbonaro V, Okkenhaug K. PI3Kδ Regulates the Magnitude of CD8+ T Cell Responses after Challenge with Listeria monocytogenes. J Immunol. 2015;195:3206-17 pubmed publisher
Fournier M, Lebert Ghali C, Bijl J. HOXA4 provides stronger engraftment potential to short-term repopulating cells than HOXB4. Stem Cells Dev. 2015;24:2413-22 pubmed publisher
Suzuki M, Morita R, Hirata Y, Shichita T, Yoshimura A. Spred1, a Suppressor of the Ras-ERK Pathway, Negatively Regulates Expansion and Function of Group 2 Innate Lymphoid Cells. J Immunol. 2015;195:1273-81 pubmed publisher
Muro R, Nitta T, Okada T, Ideta H, Tsubata T, Suzuki H. The Ras GTPase-activating protein Rasal3 supports survival of naive T cells. PLoS ONE. 2015;10:e0119898 pubmed publisher
Engels N, König L, Schulze W, Radtke D, Vanshylla K, Lutz J, et al. The immunoglobulin tail tyrosine motif upgrades memory-type BCRs by incorporating a Grb2-Btk signalling module. Nat Commun. 2014;5:5456 pubmed publisher
Ho K, Chiang Y, Lai A, Liao N, Chang Y, Yang Yen H, et al. CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling. Cell Death Dis. 2014;5:e1518 pubmed publisher
Honda H, Nagai Y, Matsunaga T, Okamoto N, Watanabe Y, Tsuneyama K, et al. Isoliquiritigenin is a potent inhibitor of NLRP3 inflammasome activation and diet-induced adipose tissue inflammation. J Leukoc Biol. 2014;96:1087-100 pubmed publisher
Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
Nihon Yanagi Y, Terai K, Murano T, Kawai T, Kimura S, Okazumi S. ?-2 microglobulin is unsuitable as an internal reference gene for the analysis of gene expression in human colorectal cancer. Biomed Rep. 2013;1:193-196 pubmed
Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X, et al. Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity. Clin Vaccine Immunol. 2014;21:156-64 pubmed publisher
Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
Liao G, Nayak S, Regueiro J, Berger S, Detre C, Romero X, et al. GITR engagement preferentially enhances proliferation of functionally competent CD4+CD25+FoxP3+ regulatory T cells. Int Immunol. 2010;22:259-70 pubmed publisher
Qi Q, Xia M, Hu J, Hicks E, Iyer A, Xiong N, et al. Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production. Blood. 2009;114:564-71 pubmed publisher
Schulteis R, Chu H, Dai X, Chen Y, Edwards B, Haribhai D, et al. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood. 2008;112:4905-14 pubmed publisher
Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol Gastrointest Liver Physiol. 2008;295:G273-84 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Chappaz S, Flueck L, Farr A, Rolink A, Finke D. Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice. Blood. 2007;110:3862-70 pubmed
Perchonock C, Pajerowski A, Nguyen C, Shapiro M, Shapiro V. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007;179:1768-75 pubmed
Perchonock C, Fernando M, Quinn W, Nguyen C, Sun J, Shapiro M, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15 pubmed
de Koning B, van Dieren J, Lindenbergh Kortleve D, Van Der Sluis M, Matsumoto T, Yamaguchi K, et al. Contributions of mucosal immune cells to methotrexate-induced mucositis. Int Immunol. 2006;18:941-9 pubmed
Curtsinger J, Lins D, Johnson C, Mescher M. Signal 3 tolerant CD8 T cells degranulate in response to antigen but lack granzyme B to mediate cytolysis. J Immunol. 2005;175:4392-9 pubmed
product information
Antigen :
CD3epsilon
Apps. Abbrev. :
Flow Cytometry
Cat # :
100304
Clone :
145-2C11
Item :
Biotin anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
500 μg
Price (USD) :
110 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
BIOTIN
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA