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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD3
catalog :
100221
quantity :
25 μg
price :
95 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
17A2
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig s4d
Abreu Mota T, Hagen K, Cooper K, Jahrling P, Tan G, Wirblich C, et al. Non-neutralizing antibodies elicited by recombinant Lassa-Rabies vaccine are critical for protection against Lassa fever. Nat Commun. 2018;9:4223 pubmed publisher
  • flow cytometry; mouse; 2.5 ug/ml; loading ...; fig s4
Schrand B, Clark E, Levay A, Capote A, Martínez O, Brenneman R, et al. Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity. Nat Commun. 2018;9:3348 pubmed publisher
  • flow cytometry; mouse
Chen J, Miyanishi M, Wang S, Yamazaki S, Sinha R, Kao K, et al. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016;530:223-7 pubmed publisher
Rohner L, Reinhart R, Iype J, Bachmann S, Kaufmann T, Fux M. Impact of BH3-mimetics on Human and Mouse Blood Leukocytes: A Comparative Study. Sci Rep. 2020;10:222 pubmed publisher
Dokoshi T, Zhang L, Li F, Nakatsuji T, Butcher A, Yoshida H, et al. Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection. Cell Rep. 2020;30:61-68.e4 pubmed publisher
Sanchez H, Moroney J, Gan H, Shen T, Im J, Li T, et al. B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids. Nat Commun. 2020;11:60 pubmed publisher
Rashidi M, Simpson D, Hempel A, Frank D, Petrie E, Vince A, et al. The Pyroptotic Cell Death Effector Gasdermin D Is Activated by Gout-Associated Uric Acid Crystals but Is Dispensable for Cell Death and IL-1β Release. J Immunol. 2019;: pubmed publisher
Choi H, Deng J, Li S, Silk T, Dong L, Brea E, et al. Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer. Cell Rep. 2019;27:806-819.e5 pubmed publisher
Adane B, Ye H, Khan N, Pei S, Minhajuddin M, Stevens B, et al. The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells. Cell Rep. 2019;27:238-254.e6 pubmed publisher
Yin S, Gambe R, Sun J, Martinez A, Cartun Z, Regis F, et al. A Murine Model of Chronic Lymphocytic Leukemia Based on B Cell-Restricted Expression of Sf3b1 Mutation and Atm Deletion. Cancer Cell. 2019;35:283-296.e5 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Tordesillas L, Lozano Ojalvo D, Dunkin D, Mondoulet L, Agudo J, Merad M, et al. PDL2+ CD11b+ dermal dendritic cells capture topical antigen through hair follicles to prime LAP+ Tregs. Nat Commun. 2018;9:5238 pubmed publisher
Durgeau A, Virk Y, Gros G, Voilin E, Corgnac S, Djenidi F, et al. Human preprocalcitonin self-antigen generates TAP-dependent and -independent epitopes triggering optimised T-cell responses toward immune-escaped tumours. Nat Commun. 2018;9:5097 pubmed publisher
Alissafi T, Hatzioannou A, Mintzas K, Barouni R, Banos A, Sormendi S, et al. Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells. J Clin Invest. 2018;128:3840-3852 pubmed publisher
Habib S, El Andaloussi A, Elmasry K, Handoussa A, Azab M, Elsawey A, et al. PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani. Infect Immun. 2018;86: pubmed publisher
Draijer C, Robbe P, Boorsma C, Hylkema M, Melgert B. Dual role of YM1+ M2 macrophages in allergic lung inflammation. Sci Rep. 2018;8:5105 pubmed publisher
Revathikumar P, Estelius J, Karmakar U, Le Maitre E, Korotkova M, Jakobsson P, et al. Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mouse spleen. PLoS ONE. 2018;13:e0193210 pubmed publisher
Haertel E, Joshi N, Hiebert P, Kopf M, Werner S. Regulatory T cells are required for normal and activin-promoted wound repair in mice. Eur J Immunol. 2018;48:1001-1013 pubmed publisher
Teater M, Domínguez P, Redmond D, Chen Z, Ennishi D, Scott D, et al. AICDA drives epigenetic heterogeneity and accelerates germinal center-derived lymphomagenesis. Nat Commun. 2018;9:222 pubmed publisher
Kunimoto H, Meydan C, Nazir A, Whitfield J, Shank K, Rapaport F, et al. Cooperative Epigenetic Remodeling by TET2 Loss and NRAS Mutation Drives Myeloid Transformation and MEK Inhibitor Sensitivity. Cancer Cell. 2018;33:44-59.e8 pubmed publisher
Kleppe M, Koche R, Zou L, van Galen P, Hill C, Dong L, et al. Dual Targeting of Oncogenic Activation and Inflammatory Signaling Increases Therapeutic Efficacy in Myeloproliferative Neoplasms. Cancer Cell. 2018;33:29-43.e7 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Zhou Y, Weng X, Dou R, Tan X, Zhang T, Fang J, et al. Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice. Acta Pharmacol Sin. 2017;38:201-210 pubmed publisher
Kojima T, Tsuchiya K, Ikemizu S, Yoshikawa S, Yamanishi Y, Watanabe M, et al. Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R. Sci Rep. 2016;6:36457 pubmed publisher
Di Marco Barros R, Roberts N, Dart R, Vantourout P, Jandke A, Nussbaumer O, et al. Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments. Cell. 2016;167:203-218.e17 pubmed publisher
Wen J, Wang X, Pei H, Xie C, Qiu N, Li S, et al. Anti-psoriatic effects of Honokiol through the inhibition of NF-κB and VEGFR-2 in animal model of K14-VEGF transgenic mouse. J Pharmacol Sci. 2015;128:116-24 pubmed publisher
Voss M, Wolf L, Kamyschnikow A, Wonnenberg B, Honecker A, Herr C, et al. Il-17A contributes to maintenance of pulmonary homeostasis in a murine model of cigarette smoke-induced emphysema. Am J Physiol Lung Cell Mol Physiol. 2015;309:L188-95 pubmed publisher
Chung Y, Kim E, Abdel Wahab O. Femoral bone marrow aspiration in live mice. J Vis Exp. 2014;: pubmed publisher
Mathews J, Williams A, Brand J, Wurmbrand A, Chen L, Ninin F, et al. γδ T cells are required for pulmonary IL-17A expression after ozone exposure in mice: role of TNFα. PLoS ONE. 2014;9:e97707 pubmed publisher
Jie Z, Liang Y, Hou L, Dong C, Iwakura Y, Soong L, et al. Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection. J Immunol. 2014;192:3289-300 pubmed publisher
Spees A, Kingsbury D, Wangdi T, Xavier M, Tsolis R, BAUMLER A. Neutrophils are a source of gamma interferon during acute Salmonella enterica serovar Typhimurium colitis. Infect Immun. 2014;82:1692-7 pubmed publisher
Xiao J, Angsana J, Wen J, Smith S, Park P, Ford M, et al. Syndecan-1 displays a protective role in aortic aneurysm formation by modulating T cell-mediated responses. Arterioscler Thromb Vasc Biol. 2012;32:386-96 pubmed publisher
Morgado P, Ong Y, Boothroyd J, Lodoen M. Toxoplasma gondii induces B7-2 expression through activation of JNK signal transduction. Infect Immun. 2011;79:4401-12 pubmed publisher
product information
Antigen :
CD3
Apps. Abbrev. :
Flow Cytometry
Cat # :
100221
Clone :
17A2
Item :
APC/Cyanine7 anti-mouse CD3
Isotype :
Rat IgG2b, κ
Other Names :
T cell antigen receptor complex, T3
Size :
25 μg
Price (USD) :
95 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
γδTCR-positive T-T hybridoma D1
Application Notes :
The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3 + cells in vivo . Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , complement-mediated cytotoxicity 1,3 , immunohistochemical staining of acetone-fixed frozen sections 1,4 , in vitro stimulation of T cells 1 and depletion of CD3 + cells in vivo 2 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA