product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse CD3
catalog :
100214
quantity :
100 μg
price :
195 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
17A2
reactivity :
mouse
application :
immunohistochemistry, flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 3c
Sitrin J, Suto E, Wuster A, Eastham Anderson J, Kim J, Austin C, et al. The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice. J Immunol. 2017;199:1238-1249 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8a
Griffiths K, Dolezal O, Cao B, Nilsson S, See H, Pfleger K, et al. i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016;291:12641-57 pubmed publisher
  • flow cytometry; mouse
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
Bitschar K, Sauer B, Focken J, Dehmer H, Moos S, Konnerth M, et al. Lugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors. Nat Commun. 2019;10:2730 pubmed publisher
van Erp E, Lakerveld A, Mulder H, Luytjes W, Ferwerda G, van Kasteren P. Pathogenesis of Respiratory Syncytial Virus Infection in BALB/c Mice Differs Between Intratracheal and Intranasal Inoculation. Viruses. 2019;11: pubmed publisher
Nowlan B, Williams E, Doran M, Levesque J. CD27, CD201, FLT3, CD48, and CD150 cell surface staining identifies long-term mouse hematopoietic stem cells in immunodeficient non-obese diabetic severe combined immune deficient derived strains. Haematologica. 2019;: pubmed publisher
Lasso P, Llano Murcia M, Sandoval T, Urueña C, Barreto A, Fiorentino S. Breast Tumor Cells Highly Resistant to Drugs Are Controlled Only by the Immune Response Induced in an Immunocompetent Mouse Model. Integr Cancer Ther. 2019;18:1534735419848047 pubmed publisher
Choi H, Deng J, Li S, Silk T, Dong L, Brea E, et al. Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer. Cell Rep. 2019;27:806-819.e5 pubmed publisher
Thompson P, Shah A, Ntranos V, Van Gool F, Atkinson M, Bhushan A. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes. Cell Metab. 2019;29:1045-1060.e10 pubmed publisher
Koh K, Cao Y, Mangos S, Tardi N, Dande R, Lee H, et al. Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist. J Clin Invest. 2019;129:1713-1726 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Cuburu N, Kim R, Guittard G, Thompson C, Day P, Hamm D, et al. A Prime-Pull-Amplify Vaccination Strategy To Maximize Induction of Circulating and Genital-Resident Intraepithelial CD8+ Memory T Cells. J Immunol. 2019;202:1250-1264 pubmed publisher
Wang X, Piersma S, Nelson C, Dai Y, Christensen T, Lazear E, et al. A herpesvirus encoded Qa-1 mimic inhibits natural killer cell cytotoxicity through CD94/NKG2A receptor engagement. elife. 2018;7: pubmed publisher
Peer S, Cappellano G, Hermann Kleiter N, Albrecht Schgoer K, Hinterleitner R, Baier G, et al. Regulation of Lymphatic GM-CSF Expression by the E3 Ubiquitin Ligase Cbl-b. Front Immunol. 2018;9:2311 pubmed publisher
Li C, DiSpirito J, Zemmour D, Spallanzani R, Kuswanto W, Benoist C, et al. TCR Transgenic Mice Reveal Stepwise, Multi-site Acquisition of the Distinctive Fat-Treg Phenotype. Cell. 2018;174:285-299.e12 pubmed publisher
Schneider C, O Leary C, von Moltke J, Liang H, Ang Q, Turnbaugh P, et al. A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling. Cell. 2018;174:271-284.e14 pubmed publisher
Liu Q, Ma W, Yang J, Zhao Z, Yan K, Yao Y, et al. The CXC Chemokine Receptor 3 Inhibits Autoimmune Cholangitis via CD8+ T Cells but Promotes Colitis via CD4+ T Cells. Front Immunol. 2018;9:1090 pubmed publisher
Trompette A, Gollwitzer E, Pattaroni C, Lopez Mejia I, Riva E, Pernot J, et al. Dietary Fiber Confers Protection against Flu by Shaping Ly6c- Patrolling Monocyte Hematopoiesis and CD8+ T Cell Metabolism. Immunity. 2018;48:992-1005.e8 pubmed publisher
Divan A, Casselli T, Narayanan S, Mukherjee S, Zawieja D, Watt J, et al. Borrelia burgdorferi adhere to blood vessels in the dura mater and are associated with increased meningeal T cells during murine disseminated borreliosis. PLoS ONE. 2018;13:e0196893 pubmed publisher
García Rodríguez S, Rosal Vela A, Botta D, Cumba Garcia L, Zumaquero E, Prados Maniviesa V, et al. CD38 promotes pristane-induced chronic inflammation and increases susceptibility to experimental lupus by an apoptosis-driven and TRPM2-dependent mechanism. Sci Rep. 2018;8:3357 pubmed publisher
Ma W, Liu Q, Yang J, Yang Y, Zhao Z, Ma H, et al. A Mouse Model of Autoimmune Cholangitis via Syngeneic Bile Duct Protein Immunization. Sci Rep. 2017;7:15246 pubmed publisher
Nair S, Poddar S, Shimak R, Diamond M. Interferon Regulatory Factor 1 Protects against Chikungunya Virus-Induced Immunopathology by Restricting Infection in Muscle Cells. J Virol. 2017;91: pubmed publisher
Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, et al. T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity. 2017;47:349-362.e5 pubmed publisher
Ma W, Jia Y, Liu Q, Yang Y, Yang J, Zhao Z, et al. Modulation of liver regeneration via myeloid PTEN deficiency. Cell Death Dis. 2017;8:e2827 pubmed publisher
Chen S, Huang A, Chen H, Yang Y, Xia F, Jin L, et al. miR-34a inhibits the apoptosis of MDSCs by suppressing the expression of N-myc. Immunol Cell Biol. 2016;94:563-72 pubmed publisher
Nagaoka M, Hatta Y, Kawaoka Y, Malherbe L. Antigen signal strength during priming determines effector CD4 T cell function and antigen sensitivity during influenza virus challenge. J Immunol. 2014;193:2812-20 pubmed publisher
Christian D, Koshy A, Reuter M, Betts M, Boothroyd J, Hunter C. Use of transgenic parasites and host reporters to dissect events that promote interleukin-12 production during toxoplasmosis. Infect Immun. 2014;82:4056-67 pubmed publisher
Shiomi A, Usui T, Ishikawa Y, Shimizu M, Murakami K, Mimori T. GM-CSF but not IL-17 is critical for the development of severe interstitial lung disease in SKG mice. J Immunol. 2014;193:849-59 pubmed publisher
O E, Lee Y, Ko E, Kim K, Lee Y, Song J, et al. Roles of major histocompatibility complex class II in inducing protective immune responses to influenza vaccination. J Virol. 2014;88:7764-75 pubmed publisher
Brenndörfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol. 2014;192:1671-80 pubmed publisher
Mizraji G, Segev H, Wilensky A, Hovav A. Isolation, processing and analysis of murine gingival cells. J Vis Exp. 2013;:e50388 pubmed publisher
Neff T, Sinha A, Kluk M, Zhu N, Khattab M, Stein L, et al. Polycomb repressive complex 2 is required for MLL-AF9 leukemia. Proc Natl Acad Sci U S A. 2012;109:5028-33 pubmed publisher
Xiao J, Angsana J, Wen J, Smith S, Park P, Ford M, et al. Syndecan-1 displays a protective role in aortic aneurysm formation by modulating T cell-mediated responses. Arterioscler Thromb Vasc Biol. 2012;32:386-96 pubmed publisher
Morgado P, Ong Y, Boothroyd J, Lodoen M. Toxoplasma gondii induces B7-2 expression through activation of JNK signal transduction. Infect Immun. 2011;79:4401-12 pubmed publisher
de Lalla C, Rinaldi A, Montagna D, Azzimonti L, Bernardo M, Sangalli L, et al. Invariant NKT cell reconstitution in pediatric leukemia patients given HLA-haploidentical stem cell transplantation defines distinct CD4+ and CD4- subset dynamics and correlates with remission state. J Immunol. 2011;186:4490-9 pubmed publisher
Chiossone L, Chaix J, Fuseri N, Roth C, Vivier E, Walzer T. Maturation of mouse NK cells is a 4-stage developmental program. Blood. 2009;113:5488-96 pubmed publisher
Achtman A, Höpken U, Bernert C, Lipp M. CCR7-deficient mice develop atypically persistent germinal centers in response to thymus-independent type 2 antigens. J Leukoc Biol. 2009;85:409-17 pubmed publisher
product information
Antigen :
CD3
Apps. Abbrev. :
Flow Cytometry
Cat # :
100214
Clone :
17A2
Item :
Pacific Blue™ anti-mouse CD3
Isotype :
Rat IgG2b, κ
Other Names :
T cell antigen receptor complex, T3
Size :
100 μg
Price (USD) :
195 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
γδTCR-positive T-T hybridoma D1
Application Notes :
The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3 + cells in vivo . Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , complement-mediated cytotoxicity 1,3 , immunohistochemical staining of acetone-fixed frozen sections 1,4 , in vitro stimulation of T cells 1 and depletion of CD3 + cells in vivo 2 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA