product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti Tubulin Alpha
catalog :
MCA78G
quantity :
0.5 mg
price :
544 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
YOL1/34
reactivity :
Saccharomycetales, human, mouse, rat, brewer's yeast, roundworm
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 1f
  • immunocytochemistry; human; 1:1000; loading ...; fig 1d
Liccardi G, Ramos Garcia L, Tenev T, Annibaldi A, Legrand A, Robertson D, et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell. 2019;73:413-428.e7 pubmed publisher
  • immunocytochemistry; roundworm ; fig 2
  • immunocytochemistry; human; fig 1
Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
  • immunocytochemistry; rat; 1:1000; fig 3
  • western blot; rat; 1:1000
Uehara R, Kamasaki T, Hiruma S, Poser I, Yoda K, Yajima J, et al. Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission. Mol Biol Cell. 2016;27:812-27 pubmed publisher
  • immunocytochemistry; human; 1:3000
Zielinska A, Holubcová Z, Blayney M, Elder K, Schuh M. Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect. elife. 2015;4:e11389 pubmed publisher
  • immunohistochemistry; mouse; 1:3000; fig s2
Clift D, Schuh M. A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes. Nat Commun. 2015;6:7217 pubmed publisher
  • immunocytochemistry; Saccharomycetales
Tsai F, Vijayraghavan S, Prinz J, MacAlpine H, MacAlpine D, Schwacha A. Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate. Mol Cell Biol. 2015;35:2131-43 pubmed publisher
  • flow cytometry; brewer's yeast; fig s3
Iacovella M, Golfieri C, Massari L, Busnelli S, Pagliuca C, Dal Maschio M, et al. Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation. Nat Commun. 2015;6:6643 pubmed publisher
  • western blot; brewer's yeast; fig s1
Sánchez de Groot N, Gomes R, Villar Piqué A, Babu M, Coelho A, Ventura S. Proteome response at the edge of protein aggregation. Open Biol. 2015;5:140221 pubmed publisher
  • immunocytochemistry; human
Hasegawa H, Ishibashi K, Kubota S, Yamaguchi C, Yuki R, Nakajo H, et al. Cdk1-mediated phosphorylation of human ATF7 at Thr-51 and Thr-53 promotes cell-cycle progression into M phase. PLoS ONE. 2014;9:e116048 pubmed publisher
  • immunocytochemistry; human
Sarbajna S, Davies D, West S. Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe. Genes Dev. 2014;28:1124-36 pubmed publisher
  • western blot; human
Bersten D, Bruning J, Peet D, Whitelaw M. Human variants in the neuronal basic helix-loop-helix/Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. PLoS ONE. 2014;9:e85768 pubmed publisher
  • western blot; mouse
  • western blot; human
Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
Scheffler K, Uraji J, Jentoft I, Cavazza T, Mönnich E, Mogessie B, et al. Two mechanisms drive pronuclear migration in mouse zygotes. Nat Commun. 2021;12:841 pubmed publisher
Jühlen R, Martinelli V, Vinci C, Breckpot J, Fahrenkrog B. Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts. Sci Rep. 2020;10:19301 pubmed publisher
Zielinska A, Bellou E, Sharma N, Frombach A, Seres K, Gruhn J, et al. Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs. Curr Biol. 2019;29:3749-3765.e7 pubmed publisher
Adamakis I, Panteris E, Eleftheriou E. Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum. Biomolecules. 2019;9: pubmed publisher
Karasu M, Bouftas N, Keeney S, Wassmann K. Cyclin B3 promotes anaphase I onset in oocyte meiosis. J Cell Biol. 2019;218:1265-1281 pubmed publisher
Sawyer E, Joshi P, Jorgensen V, Yunus J, Berchowitz L, Unal E. Developmental regulation of an organelle tether coordinates mitochondrial remodeling in meiosis. J Cell Biol. 2019;218:559-579 pubmed publisher
Fink S, Zugelder L, Roth B, Brandt E, Meloche S, Izsvák Z, et al. A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors. PLoS ONE. 2018;13:e0205585 pubmed publisher
Yamamoto Hino M, Katsumata E, Suzuki E, Maeda Y, Kinoshita T, Goto S. Nuclear envelope localization of PIG-B is essential for GPI-anchor synthesis in Drosophila. J Cell Sci. 2018;131: pubmed publisher
Horiuchi M, Kuga T, Saito Y, Nagano M, Adachi J, Tomonaga T, et al. The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1. J Biol Chem. 2018;293:15524-15537 pubmed publisher
Balmus G, Larrieu D, Barros A, Collins C, Abrudan M, Demir M, et al. Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome. Nat Commun. 2018;9:1700 pubmed publisher
Parker F, Batchelor M, Wolny M, Hughes R, Knight P, Peckham M. A1603P and K1617del, Mutations in β-Cardiac Myosin Heavy Chain that Cause Laing Early-Onset Distal Myopathy, Affect Secondary Structure and Filament Formation In Vitro and In Vivo. J Mol Biol. 2018;430:1459-1478 pubmed publisher
Wang S, Stoops E, Cp U, Markus B, Reuveny A, Ordan E, et al. Mechanotransduction via the LINC complex regulates DNA replication in myonuclei. J Cell Biol. 2018;217:2005-2018 pubmed publisher
Touati S, Kataria M, Jones A, Snijders A, Uhlmann F. Phosphoproteome dynamics during mitotic exit in budding yeast. EMBO J. 2018;37: pubmed publisher
Novák D, Vadovic P, Ovecka M, Samajová O, Komis G, Colcombet J, et al. Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy. Front Plant Sci. 2018;9:371 pubmed publisher
Veri A, Miao Z, Shapiro R, Tebbji F, O Meara T, Kim S, et al. Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space. PLoS Genet. 2018;14:e1007270 pubmed publisher
Murayama Y, Samora C, Kurokawa Y, Iwasaki H, Uhlmann F. Establishment of DNA-DNA Interactions by the Cohesin Ring. Cell. 2018;172:465-477.e15 pubmed publisher
Vaškebová L, Samaj J, Ovecka M. Single-point ACT2 gene mutation in the Arabidopsis root hair mutant der1-3 affects overall actin organization, root growth and plant development. Ann Bot. 2018;122:889-901 pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
Tungadi E, Ito A, Kiyomitsu T, Goshima G. Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. J Cell Sci. 2017;130:3676-3684 pubmed publisher
Carvalhal S, Stevense M, Koehler K, Naumann R, Huebner A, Jessberger R, et al. ALADIN is required for the production of fertile mouse oocytes. Mol Biol Cell. 2017;28:2470-2478 pubmed publisher
Herwald S, Zucchi P, Tan S, Kumamoto C. The two transmembrane regions of Candida albicans Dfi1 contribute to its biogenesis. Biochem Biophys Res Commun. 2017;488:153-158 pubmed publisher
Lucchetta E, Ohlstein B. Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine. Cell Stem Cell. 2017;20:609-620.e6 pubmed publisher
Morii M, Kubota S, Honda T, Yuki R, Morinaga T, Kuga T, et al. Src Acts as an Effector for Ku70-dependent Suppression of Apoptosis through Phosphorylation of Ku70 at Tyr-530. J Biol Chem. 2017;292:1648-1665 pubmed publisher
Chen C, Lim H, Shi J, Tamura S, Maeshima K, Surana U, et al. Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo. Mol Biol Cell. 2016;27:3357-3368 pubmed
Baumann J, Wong J, Sun Y, Conklin D. Palmitate-induced ER stress increases trastuzumab sensitivity in HER2/neu-positive breast cancer cells. BMC Cancer. 2016;16:551 pubmed publisher
O Meara T, Veri A, Polvi E, Li X, Valaei S, Diezmann S, et al. Mapping the Hsp90 Genetic Network Reveals Ergosterol Biosynthesis and Phosphatidylinositol-4-Kinase Signaling as Core Circuitry Governing Cellular Stress. PLoS Genet. 2016;12:e1006142 pubmed publisher
Bersten D, Sullivan A, Li D, Bhakti V, Bent S, Whitelaw M. Inducible and reversible lentiviral and Recombination Mediated Cassette Exchange (RMCE) systems for controlling gene expression. PLoS ONE. 2015;10:e0116373 pubmed publisher
Tape C, Norrie I, Worboys J, Lim L, Lauffenburger D, Jørgensen C. Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture. Mol Cell Proteomics. 2014;13:1866-76 pubmed publisher
Sullivan A, Raimondo A, Schwab T, Bruning J, Froguel P, Farooqi I, et al. Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2. Biochem J. 2014;461:403-12 pubmed publisher
Nakayama Y, Saito Y, Soeda S, Iwamoto E, Ogawa S, Yamagishi N, et al. Genistein induces cytokinesis failure through RhoA delocalization and anaphase chromosome bridging. J Cell Biochem. 2014;115:763-71 pubmed publisher
Hammond S, Byrum S, Namjoshi S, Graves H, Dennehey B, Tackett A, et al. Mitotic phosphorylation of histone H3 threonine 80. Cell Cycle. 2014;13:440-52 pubmed publisher
Aoyama K, Yuki R, Horiike Y, Kubota S, Yamaguchi N, Morii M, et al. Formation of long and winding nuclear F-actin bundles by nuclear c-Abl tyrosine kinase. Exp Cell Res. 2013;319:3251-68 pubmed publisher
Zhang A, Liu Z, Myers L. Differential regulation of white-opaque switching by individual subunits of Candida albicans mediator. Eukaryot Cell. 2013;12:1293-304 pubmed publisher
Ramachandrappa S, Raimondo A, Cali A, Keogh J, Henning E, Saeed S, et al. Rare variants in single-minded 1 (SIM1) are associated with severe obesity. J Clin Invest. 2013;123:3042-50 pubmed publisher
Hao N, Bhakti V, Peet D, Whitelaw M. Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation. Nucleic Acids Res. 2013;41:5626-38 pubmed publisher
Soeda S, Nakayama Y, Honda T, Aoki A, Tamura N, Abe K, et al. v-Src causes delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone during cytokinesis failure. Exp Cell Res. 2013;319:1382-97 pubmed publisher
Bürstenbinder K, Savchenko T, Muller J, Adamson A, Stamm G, Kwong R, et al. Arabidopsis calmodulin-binding protein IQ67-domain 1 localizes to microtubules and interacts with kinesin light chain-related protein-1. J Biol Chem. 2013;288:1871-82 pubmed publisher
Robbins N, Leach M, Cowen L. Lysine deacetylases Hda1 and Rpd3 regulate Hsp90 function thereby governing fungal drug resistance. Cell Rep. 2012;2:878-88 pubmed publisher
González Santamaría J, Campagna M, Ortega Molina A, Marcos Villar L, de la Cruz Herrera C, Gonzalez D, et al. Regulation of the tumor suppressor PTEN by SUMO. Cell Death Dis. 2012;3:e393 pubmed publisher
Hao N, Lee K, Furness S, Bosdotter C, Poellinger L, Whitelaw M. Xenobiotics and loss of cell adhesion drive distinct transcriptional outcomes by aryl hydrocarbon receptor signaling. Mol Pharmacol. 2012;82:1082-93 pubmed publisher
Nakayama Y, Matsui Y, Takeda Y, Okamoto M, Abe K, Fukumoto Y, et al. c-Src but not Fyn promotes proper spindle orientation in early prometaphase. J Biol Chem. 2012;287:24905-15 pubmed publisher
Walsh C. The structure of the mitotic spindle and nucleolus during mitosis in the amebo-flagellate Naegleria. PLoS ONE. 2012;7:e34763 pubmed publisher
Sato H, Yamada R, Yanagihara M, Okuzawa H, Iwata H, Kurosawa A, et al. New 2-aryl-1,4-naphthoquinone-1-oxime methyl ether compound induces microtubule depolymerization and subsequent apoptosis. J Pharmacol Sci. 2012;118:467-78 pubmed
Aoyama K, Fukumoto Y, Ishibashi K, Kubota S, Morinaga T, Horiike Y, et al. Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp Cell Res. 2011;317:2874-903 pubmed publisher
Robbins N, Uppuluri P, Nett J, Rajendran R, Ramage G, Lopez Ribot J, et al. Hsp90 governs dispersion and drug resistance of fungal biofilms. PLoS Pathog. 2011;7:e1002257 pubmed publisher
Keeling J, Miller R. Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast. Methods Mol Biol. 2011;782:231-44 pubmed publisher
Hao N, Whitelaw M, Shearwin K, Dodd I, Chapman Smith A. Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR. Nucleic Acids Res. 2011;39:3695-709 pubmed publisher
To S, Rodda S, Rathjen P, Keough R. Modulation of CP2 family transcriptional activity by CRTR-1 and sumoylation. PLoS ONE. 2010;5:e11702 pubmed publisher
Whelan F, Hao N, Furness S, Whitelaw M, Chapman Smith A. Amino acid substitutions in the aryl hydrocarbon receptor ligand binding domain reveal YH439 as an atypical AhR activator. Mol Pharmacol. 2010;77:1037-46 pubmed publisher
Higashiyama Y, Takahashi A, Fukumoto Y, Nakayama Y, Yamaguchi N. Induction of chromatin condensation by nuclear expression of a novel arginine-rich cationic protein genetically engineered from the enhanced green fluorescent protein. Cytotechnology. 2009;60:153-9 pubmed publisher
Sdralia N, Patmanidi A, Velentzas A, Margaritis L, Baltatzis G, Hatzinikolaou D, et al. The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent. Respir Res. 2009;10:82 pubmed publisher
Nakayama Y, Igarashi A, Kikuchi I, Obata Y, Fukumoto Y, Yamaguchi N. Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp Cell Res. 2009;315:2515-28 pubmed publisher
Hartl T, Sweeney S, Knepler P, Bosco G. Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis. PLoS Genet. 2008;4:e1000228 pubmed publisher
Kuga T, Hoshino M, Nakayama Y, Kasahara K, Ikeda K, Obata Y, et al. Role of Src-family kinases in formation of the cortical actin cap at the dorsal cell surface. Exp Cell Res. 2008;314:2040-54 pubmed publisher
Kuga T, Nakayama Y, Hoshino M, Higashiyama Y, Obata Y, Matsuda D, et al. Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells. Arch Biochem Biophys. 2007;466:116-24 pubmed
Yu H, Koshland D. The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis. J Cell Biol. 2007;176:911-8 pubmed
Kateneva A, Konovchenko A, Guacci V, Dresser M. Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae. J Cell Biol. 2005;171:241-53 pubmed
White J, Stead E, Faast R, Conn S, Cartwright P, Dalton S. Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation. Mol Biol Cell. 2005;16:2018-27 pubmed
Lavoie B, Hogan E, Koshland D. In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin. J Cell Biol. 2002;156:805-15 pubmed
image
image 1 :
Bio-Rad MCA78G image 1
Published customer image: . Rat anti tubulin alpha antibody, clone YOL1/34 ( MCA78G ) used as a loading control for the detection of tubulin- in Candida albicans lysates by western blotting. Image caption: . Staurosporine induces filamentation independent of Nrg1 degradation. (A) Staurosporine-induced filamentation is not accompanied by Nrg1 protein degradation. SN95 wild-type cells expressing native levels of HA-tagged Nrg1 were grown in YPD plus 10% serum at 37 C, YPD plus 200 mM hydroxyurea (HU) at 30 C, or YPD plus 0.5 ug/ml staurosporine at 30 C for 5 or 65 min. Total proteins were resolved by SDS-PAGE gel, and the blot was hybridized with anti-HA to detect Nrg1 and anti-tubulin to monitor tubulin as a loading control. (B) Staurosporine induces germ tube formation at different kinetics from serum and hydroxyurea. Cells were imaged by DIC microscopy. The scale bar indicates 10 um. From: Xie JL, O'Meara TR, Polvi EJ, Robbins N, Cowen LE. Staurosporine Induces Filamentation in the Human Fungal Pathogen Candida albicans via Signaling through Cyr1 and Protein Kinase A. mSphere. 2017 Mar 1;2(2). pii: e00056-17. eCollection 2017 Mar-Apr .
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA78G
Description :
Rat anti Tubulin Alpha
Specificity :
Tubulin Alpha
TargetSpecies :
Yeast
Host :
Rat
Format :
Purified
Isotypes :
IgG2a
Applications :
WB,IF,C,E,R
ApplicationTypes :
Western Blotting,Immunofluorescence,Immunohistology - Frozen,ELISA,Radioimmunoassays
Cross Reactivities :
Birds,Mammals,Drosophila,Fungal,Human,Arabidopsis thaliana,Saccharomyces,Platyzoma ,Ashbya,Mouse,Naegleria,Asplenium nidus,Seagrass (Cymodocea nodosa),Rye (Secale cereale L.)
Clone :
YOL1/34
Quantity :
0.5 mg
Quantity Value :
0.5
Quantity Type :
mg
Entity Formats :
Purified
UNSPSC :
41116161
Concentration :
IgG concentration 1.0mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
388
PriceEUR :
544
PriceUSD :
544 USD
PriceCHF :
592
PriceSEK :
5930
PriceNOK :
5959
PriceDKK :
4056
more info or order :
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.