product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti Tubulin Alpha
catalog :
MCA77G
quantity :
0.5 mg
price :
561 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
YL1/2
reactivity :
Aglantha digitale, coelenterates , Beroe, Pleurobrachia bachei, human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 4c
Moon H, Hippenmeyer S, Luo L, Wynshaw Boris A. LIS1 determines cleavage plane positioning by regulating actomyosin-mediated cell membrane contractility. elife. 2020;9: pubmed publisher
  • immunohistochemistry; Aglantha digitale; fig 2a
Norekian T, Moroz L. Atlas of the neuromuscular system in the Trachymedusa Aglantha digitale: Insights from the advanced hydrozoan. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry; coelenterates ; loading ...; fig 2
Norekian T, Moroz L. Comparative neuroanatomy of ctenophores: Neural and muscular systems in Euplokamis dunlapae and related species. J Comp Neurol. 2020;528:481-501 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig s5
Akera T, Trimm E, Lampson M. Molecular Strategies of Meiotic Cheating by Selfish Centromeres. Cell. 2019;178:1132-1144.e10 pubmed publisher
  • immunohistochemistry; Beroe; fig 5a
Norekian T, Moroz L. Neural system and receptor diversity in the ctenophore Beroe abyssicola. J Comp Neurol. 2019;527:1986-2008 pubmed publisher
  • immunohistochemistry; Pleurobrachia bachei; 1:40; fig 7
Norekian T, Moroz L. Neuromuscular organization of the Ctenophore Pleurobrachia bachei. J Comp Neurol. 2019;527:406-436 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2h
Gholkar A, Cheung K, Williams K, Lo Y, Hamideh S, Nnebe C, et al. Fatostatin Inhibits Cancer Cell Proliferation by Affecting Mitotic Microtubule Spindle Assembly and Cell Division. J Biol Chem. 2016;291:17001-8 pubmed publisher
  • western blot; human; fig S3
Reinardy J, Corey D, Golzio C, Mueller S, Katsanis N, Kontos C. Phosphorylation of Threonine 794 on Tie1 by Rac1/PAK1 Reveals a Novel Angiogenesis Regulatory Pathway. PLoS ONE. 2015;10:e0139614 pubmed publisher
  • immunocytochemistry; human; 1:250
Ong C, Gierke S, Pitt C, Sagolla M, Cheng C, Zhou W, et al. Small molecule inhibition of group I p21-activated kinases in breast cancer induces apoptosis and potentiates the activity of microtubule stabilizing agents. Breast Cancer Res. 2015;17:59 pubmed publisher
  • immunoprecipitation; human
Geissler A, Haun F, Frank D, Wieland K, Simon M, Idzko M, et al. Apoptosis induced by the fungal pathogen gliotoxin requires a triple phosphorylation of Bim by JNK. Cell Death Differ. 2013;20:1317-29 pubmed publisher
  • western blot; human
De Faveri L, Hurst C, Platt F, Taylor C, Roulson J, Sanchez Carbayo M, et al. Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer. Br J Cancer. 2013;108:1368-77 pubmed publisher
Bernkopf D, Brückner M, Hadjihannas M, Behrens J. An aggregon in conductin/axin2 regulates Wnt/β-catenin signaling and holds potential for cancer therapy. Nat Commun. 2019;10:4251 pubmed publisher
Iida T, Kobayashi T. RNA Polymerase I Activators Count and Adjust Ribosomal RNA Gene Copy Number. Mol Cell. 2019;73:645-654.e13 pubmed publisher
Hussain S, Turnbull M, Wise H, Jagger B, Beard P, Kovacikova K, et al. Mutation of Influenza A Virus PA-X Decreases Pathogenicity in Chicken Embryos and Can Increase the Yield of Reassortant Candidate Vaccine Viruses. J Virol. 2019;93: pubmed publisher
Schubert F, Rapp J, Brauns Schubert P, Schlicher L, Stock K, Wissler M, et al. Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling. Cell Death Dis. 2018;9:470 pubmed publisher
Wojciech S, Ahmad R, Belaid Choucair Z, Journé A, Gallet S, Dam J, et al. The orphan GPR50 receptor promotes constitutive TGFβ receptor signaling and protects against cancer development. Nat Commun. 2018;9:1216 pubmed publisher
Shen E, Chen H, Ozturk A, Tu S, Shirayama M, Tang W, et al. Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline. Cell. 2018;172:937-951.e18 pubmed publisher
Bernkopf D, Jalal K, Brückner M, Knaup K, Gentzel M, Schambony A, et al. Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling. J Cell Biol. 2018;217:1383-1394 pubmed publisher
Abeysundara N, Simmonds A, Hughes S. Moesin is involved in polarity maintenance and cortical remodeling during asymmetric cell division. Mol Biol Cell. 2018;29:419-434 pubmed publisher
Jenkins B, Saunders H, Record H, Johnson Schlitz D, Wildonger J. Effects of mutating ?-tubulin lysine 40 on sensory dendrite development. J Cell Sci. 2017;130:4120-4131 pubmed publisher
Kleiner R, Hang L, Molloy K, Chait B, Kapoor T. A Chemical Proteomics Approach to Reveal Direct Protein-Protein Interactions in Living Cells. Cell Chem Biol. 2018;25:110-120.e3 pubmed publisher
Graessl M, Koch J, Calderon A, Kamps D, Banerjee S, Mazel T, et al. An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns. J Cell Biol. 2017;216:4271-4285 pubmed publisher
Pitaval A, Senger F, Letort G, Gidrol X, Guyon L, Sillibourne J, et al. Microtubule stabilization drives 3D centrosome migration to initiate primary ciliogenesis. J Cell Biol. 2017;216:3713-3728 pubmed publisher
Gao L, Yang Z, Hiremath C, Zimmerman S, Long B, BRAKEMAN P, et al. Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017;144:3511-3520 pubmed publisher
Leduc C, Etienne Manneville S. Regulation of microtubule-associated motors drives intermediate filament network polarization. J Cell Biol. 2017;216:1689-1703 pubmed publisher
Korotkevich E, Niwayama R, Courtois A, Friese S, Berger N, Buchholz F, et al. The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo. Dev Cell. 2017;40:235-247.e7 pubmed publisher
Gaudet A, Mandrekar Colucci S, Hall J, Sweet D, Schmitt P, Xu X, et al. miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair. J Neurosci. 2016;36:8516-32 pubmed publisher
Higashide M, Shinohara M. Budding Yeast SLX4 Contributes to the Appropriate Distribution of Crossovers and Meiotic Double-Strand Break Formation on Bivalents During Meiosis. G3 (Bethesda). 2016;6:2033-42 pubmed publisher
Broadhead M, Horrocks M, Zhu F, Muresan L, Benavides Piccione R, DeFelipe J, et al. PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits. Sci Rep. 2016;6:24626 pubmed publisher
Iwasaki D, Hayashihara K, Shima H, Higashide M, Terasawa M, Gasser S, et al. The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA-Dependent Tel1 Activation. PLoS Genet. 2016;12:e1005942 pubmed publisher
Postiglione I, Chiaviello A, Barra F, Roscetto E, Soriano A, Catania M, et al. Mitochondrial Malfunctioning, Proteasome Arrest and Apoptosis in Cancer Cells by Focused Intracellular Generation of Oxygen Radicals. Int J Mol Sci. 2015;16:20375-91 pubmed publisher
Gaudet A, Sweet D, Polinski N, Guan Z, Popovich P. Galectin-1 in injured rat spinal cord: implications for macrophage phagocytosis and neural repair. Mol Cell Neurosci. 2015;64:84-94 pubmed publisher
Laranjeiro R, Whitmore D. Transcription factors involved in retinogenesis are co-opted by the circadian clock following photoreceptor differentiation. Development. 2014;141:2644-56 pubmed publisher
Shahbazi M, Megias D, Epifano C, Akhmanova A, Gundersen G, Fuchs E, et al. CLASP2 interacts with p120-catenin and governs microtubule dynamics at adherens junctions. J Cell Biol. 2013;203:1043-61 pubmed publisher
Perez M, Cuadros R, Pallas Bazarra N, Garcia C, Langa E, Jurado Arjona J, et al. Boronate-tau mediated uptake in neurons. J Alzheimers Dis. 2014;40:143-51 pubmed publisher
Taylor C, Platt F, Hurst C, Thygesen H, Knowles M. Frequent inactivating mutations of STAG2 in bladder cancer are associated with low tumour grade and stage and inversely related to chromosomal copy number changes. Hum Mol Genet. 2014;23:1964-74 pubmed publisher
Moser S, Bensaddek D, Ortmann B, Maure J, Mudie S, Blow J, et al. PHD1 links cell-cycle progression to oxygen sensing through hydroxylation of the centrosomal protein Cep192. Dev Cell. 2013;26:381-92 pubmed publisher
Peltzer N, Vanli G, Yang J, Widmann C. Role of mTOR, Bad, and Survivin in RasGAP Fragment N-Mediated Cell Protection. PLoS ONE. 2013;8:e68123 pubmed publisher
di Martino E, Taylor C, Roulson J, Knowles M. An integrated genomic, transcriptional and protein investigation of FGFRL1 as a putative 4p16.3 deletion target in bladder cancer. Genes Chromosomes Cancer. 2013;52:860-71 pubmed publisher
Wang W, Tay H, Soni R, Perumal G, Goll M, Macaluso F, et al. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nat Cell Biol. 2013;15:591-601 pubmed publisher
Lyle K, Corleto J, Wittmann T. Microtubule dynamics regulation contributes to endothelial morphogenesis. Bioarchitecture. 2012;2:220-7 pubmed publisher
Gomez Ferreria M, Bashkurov M, Mullin M, Gingras A, Pelletier L. CEP192 interacts physically and functionally with the K63-deubiquitinase CYLD to promote mitotic spindle assembly. Cell Cycle. 2012;11:3555-8 pubmed publisher
Courtois A, Schuh M, Ellenberg J, Hiiragi T. The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. J Cell Biol. 2012;198:357-70 pubmed publisher
Brüning Richardson A, Bond J, Alsiary R, Richardson J, Cairns D, McCormac L, et al. NuMA overexpression in epithelial ovarian cancer. PLoS ONE. 2012;7:e38945 pubmed publisher
Tomlinson D, Baxter E, Loadman P, Hull M, Knowles M. FGFR1-induced epithelial to mesenchymal transition through MAPK/PLC?/COX-2-mediated mechanisms. PLoS ONE. 2012;7:e38972 pubmed publisher
Chiaviello A, Paciello I, Veneziani B, Palumbo G, Aloj S. Cells derived from normal or cancer breast tissue exhibit different growth properties when deprived of arginine. Med Oncol. 2012;29:2543-51 pubmed publisher
Wang S, Wu J, Suburu J, Gu Z, Cai J, Axanova L, et al. Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer. Carcinogenesis. 2012;33:404-12 pubmed publisher
Gajewski S, Comeaux E, Jafari N, Bharatham N, Bashford D, White S, et al. Analysis of the active-site mechanism of tyrosyl-DNA phosphodiesterase I: a member of the phospholipase D superfamily. J Mol Biol. 2012;415:741-58 pubmed publisher
Guaragna A, Chiaviello A, Paolella C, D Alonzo D, Palumbo G, Palumbo G. Synthesis and evaluation of folate-based chlorambucil delivery systems for tumor-targeted chemotherapy. Bioconjug Chem. 2012;23:84-96 pubmed publisher
Svobodova S, Bell S, Crump C. Analysis of the interaction between the essential herpes simplex virus 1 tegument proteins VP16 and VP1/2. J Virol. 2012;86:473-83 pubmed publisher
Ren Y, Bell S, Zenner H, Lau S, Crump C. Glycoprotein M is important for the efficient incorporation of glycoprotein H-L into herpes simplex virus type 1 particles. J Gen Virol. 2012;93:319-29 pubmed publisher
Hughes R, Gilley J, Kristiansen M, Ham J. The MEK-ERK pathway negatively regulates bim expression through the 3' UTR in sympathetic neurons. BMC Neurosci. 2011;12:69 pubmed publisher
Bakircioglu M, Carvalho O, Khurshid M, Cox J, Tuysuz B, Barak T, et al. The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis. Am J Hum Genet. 2011;88:523-35 pubmed publisher
Foeglein g, Loucaides E, Mura M, Wise H, Barclay W, Digard P. Influence of PB2 host-range determinants on the intranuclear mobility of the influenza A virus polymerase. J Gen Virol. 2011;92:1650-61 pubmed publisher
León Ortiz A, Reid R, Dittmar J, Rothstein R, Nicolas A. Srs2 overexpression reveals a helicase-independent role at replication forks that requires diverse cell functions. DNA Repair (Amst). 2011;10:506-17 pubmed publisher
Amorim M, Bruce E, Read E, Foeglein A, Mahen R, Stuart A, et al. A Rab11- and microtubule-dependent mechanism for cytoplasmic transport of influenza A virus viral RNA. J Virol. 2011;85:4143-56 pubmed publisher
Cheishvili D, Maayan C, Cohen Kupiec R, Lefler S, Weil M, Ast G, et al. IKAP/Elp1 involvement in cytoskeleton regulation and implication for familial dysautonomia. Hum Mol Genet. 2011;20:1585-94 pubmed publisher
Abe Y, Okumura E, Hosoya T, Hirota T, Kishimoto T. A single starfish Aurora kinase performs the combined functions of Aurora-A and Aurora-B in human cells. J Cell Sci. 2010;123:3978-88 pubmed publisher
Nicholas A, Khurshid M, Desir J, Carvalho O, Cox J, Thornton G, et al. WDR62 is associated with the spindle pole and is mutated in human microcephaly. Nat Genet. 2010;42:1010-4 pubmed publisher
Chiaviello A, Paciello I, Postiglione I, Crescenzi E, Palumbo G. Combination of photodynamic therapy with aspirin in human-derived lung adenocarcinoma cells affects proteasome activity and induces apoptosis. Cell Prolif. 2010;43:480-93 pubmed publisher
Mendez M, Kojima S, Goldman R. Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J. 2010;24:1838-51 pubmed publisher
Heaslip A, Ems McClung S, Hu K. TgICMAP1 is a novel microtubule binding protein in Toxoplasma gondii. PLoS ONE. 2009;4:e7406 pubmed publisher
Pawliczek T, Crump C. Herpes simplex virus type 1 production requires a functional ESCRT-III complex but is independent of TSG101 and ALIX expression. J Virol. 2009;83:11254-64 pubmed publisher
Annibaldi A, Michod D, Vanetta L, Cruchet S, Nicod P, Dubuis G, et al. Role of the sub-cellular localization of RasGAP fragment N2 for its ability to sensitize cancer cells to genotoxin-induced apoptosis. Exp Cell Res. 2009;315:2081-91 pubmed publisher
Abuhatzira L, Shemer R, Razin A. MeCP2 involvement in the regulation of neuronal alpha-tubulin production. Hum Mol Genet. 2009;18:1415-23 pubmed publisher
Hartl T, Sweeney S, Knepler P, Bosco G. Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis. PLoS Genet. 2008;4:e1000228 pubmed publisher
Wagner S, Kiupel M, Peterson R, Heikinheimo M, Wilson D. Cytochrome b5 expression in gonadectomy-induced adrenocortical neoplasms of the domestic ferret (Mustela putorius furo). Vet Pathol. 2008;45:439-42 pubmed publisher
Peters U, Cherian J, Kim J, Kwok B, Kapoor T. Probing cell-division phenotype space and Polo-like kinase function using small molecules. Nat Chem Biol. 2006;2:618-26 pubmed
Cutiño Moguel T, Fassati A. A phenotypic recessive, post-entry block in rabbit cells that results in aberrant trafficking of HIV-1. Traffic. 2006;7:978-92 pubmed
Wheeler A, Wells C, Smith S, Vega F, Henderson R, Tybulewicz V, et al. Rac1 and Rac2 regulate macrophage morphology but are not essential for migration. J Cell Sci. 2006;119:2749-57 pubmed
image
image 1 :
Bio-Rad MCA77G image 1
HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP (MCA77P)
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA77G
Description :
Rat anti Tubulin Alpha
Specificity :
Tubulin Alpha
TargetSpecies :
Yeast
Host :
Rat
Format :
Purified
Isotypes :
IgG2a
Applications :
WB,IF,IP,C,E,R
ApplicationTypes :
Western Blotting,Immunofluorescence,Immunoprecipitation,Immunohistology - Frozen,ELISA,Radioimmunoassays
Cross Reactivities :
Ashbya,Birds,Echinoderm,Human,Mouse,Dog,Rat,Pig,Drosophila,Plants,Amphibia,Saccharomyces,Pleurobrachia,Caenorhabditis,Dictyostelium discoideum,Xenopus,Pig-tailed macaque,Clytia sp.,Arabidopsis,Strongylocentrotus purpuratus,Dendraster excentricus,Trypanosoma brucei,Potorous tridactylis,Bovine,Nephrotoma suturalis ,Hemicentrotus pulcherrimus,Potato,Bombyx mori,Rhodnius prolixus,Beroe abyssicola,Candida sp.
Clone :
YL1/2
Quantity :
0.5 mg
Quantity Value :
0.5
Quantity Type :
mg
Entity Formats :
HRP,Purified,DyLight®680
UNSPSC :
41116161
Concentration :
IgG concentration 1.0 mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
400
PriceEUR :
561
PriceUSD :
561 USD
PriceCHF :
610
PriceSEK :
6395
PriceNOK :
6386
PriceDKK :
4184
more info or order :
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.