Published investigator image: . Mouse anti Pig CD28 antibody, clone 3D11( MCA6406 ) used to evaluate CD28 expression on CD4 + T cells by flow cytometry. Image caption: . Expression of CD28 in ConA-stimulated and violet proliferation dye-stained CD8+ T cells in the presence of DON and DOM-1. PBMC were cultivated and analyzed by flow cytometry under the conditions described in Figure 2. After gating on lymphocytes and live lymphocytes, CD8+ T-cells were gated within CD3+ T cells and analyzed for expression of CD28 and proliferation. Original flow cytometry data shown on the top of (b) are from one representative animal. CD28 expression was analyzed by the median fluorescence intensity (MFI) in the total population (both gates together indicated by black rectangles), the parental cell generation (gate on the right), and the proliferating cell generations (gate on the left) for CD8+ T cells (b). The boxplots summarize the results from seven animals and present the expression (MFI) of CD28 within total, parental, and proliferating ConA-stimulated CD8+ T cells (b). Different small letters on boxplots indicate significant differences. a–b: p 0.05. When small letters are not displayed above the whisker plots, no significant differences were observed (p 0.05). From: Vatzia E, Pierron A, Saalmüller A, Mayer E, Gerner W. Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets. Toxins (Basel). 2019 Nov 5;11(11):644 . doi: 10.3390/toxins11110644. This image is from an open access article distributed under terms of a Creative Commons Attribution License .