Published customer image: . Mouse anti Human factor H antibody, clone OX-23 ( MCA508G ) used as a capture reagent in a sandwich ELISA for HfH levels in murine sera with Sheep anti Human complement factor H antibody ( 4400-9504 ) as a detection reagent . Image caption: . Effect of the presence of hfH in mice on the nOMV immunogenicity. A. Evolution of hfH concentration in mouse serum over time after two IP injection of 0.5mg purified hfH (black arrows) 24 and 0.5 hours prior to immunization (grey arrow). B. Serum Bactericidal Assay (SBA) titers against 44/76-SL wild-type in pooled sera from C57bl/6 mice pre-injected with 800 μg/ml hfH or hSA, 14 days following immunization with 5 &mu:g of nOMV lpxl1 or nOMV dis . C. Individual SBA titers against 44/76-SL, dots represent individual animals D. Individual ELISA titers against rfHbp induced after one dose of H44/76 Lpxl1 OMV in mice pre-injected with hfH or hSA, *p = 0.016, not all sera were available. C, D dots represent individual animals, the geometric mean and 95% confidence interval are represented as horizontal bars. From: Daniels-Treffandier H, de Nie K, Marsay L, Dold C, Sadarangani M, Reyes-Sandoval A, et al . (2016). Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles. PLoS ONE 11(2): e0148840 .