product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti Mouse F4/80:Low Endotoxin
catalog :
MCA497EL
quantity :
0.5 mg
price :
641 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
Cl:A3-1
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Bio-Rad product webpage
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 7a
Huang Z, Liu S, Tang A, Al Rabadi L, Henkemeyer M, Mimche P, et al. Key role for EphB2 receptor in kidney fibrosis. Clin Sci (Lond). 2021;135:2127-2142 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a, s2b
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
Sorrentino C, Yin Z, Ciummo S, Lanuti P, Lu L, Marchisio M, et al. Targeting Interleukin(IL)-30/IL-27p28 signaling in cancer stem-like cells and host environment synergistically inhibits prostate cancer growth and improves survival. J Immunother Cancer. 2019;7:201 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s2a, s2b
Aarts S, Seijkens T, Kusters P, Van Tiel C, Reiche M, den Toom M, et al. Macrophage CD40 signaling drives experimental autoimmune encephalomyelitis. J Pathol. 2019;247:471-480 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1b
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch S, et al. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018;128:1852-1866 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 6h
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
A Gonzalez N, Quintana J, Garcia Silva S, Mazariegos M, González de la Aleja A, Nicolás Ávila J, et al. Phagocytosis imprints heterogeneity in tissue-resident macrophages. J Exp Med. 2017;214:1281-1296 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig s1
Lim E, Nakanishi S, Hoghooghi V, Eaton S, Palmer A, Frederick A, et al. AlphaB-crystallin regulates remyelination after peripheral nerve injury. Proc Natl Acad Sci U S A. 2017;114:E1707-E1716 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s1c
Herrtwich L, Nanda I, Evangelou K, Nikolova T, Horn V, Sagar -, et al. DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas. Cell. 2016;167:1264-1280.e18 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig S7A
Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Naeem A, Tommasi C, Cole C, Brown S, Zhu Y, Way B, et al. A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic d. J Allergy Clin Immunol. 2017;139:1228-1241 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s2
Fransén Pettersson N, Duarte N, Nilsson J, Lundholm M, Mayans S, Larefalk A, et al. A New Mouse Model That Spontaneously Develops Chronic Liver Inflammation and Fibrosis. PLoS ONE. 2016;11:e0159850 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 5
Tsuboki J, Fujiwara Y, Horlad H, Shiraishi D, Nohara T, Tayama S, et al. Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages. Sci Rep. 2016;6:29588 pubmed publisher
  • flow cytometry; mouse; fig s3
Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, et al. Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor. Sci Rep. 2016;6:26091 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Hirasawa M, Takubo K, Osada H, Miyake S, Toda E, Endo M, et al. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration. J Biol Chem. 2016;291:7373-85 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse; loading ...
Leiva M, Quintana J, Ligos J, Hidalgo A. Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability. Nat Commun. 2016;7:10222 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 4a
Ni Y, Nagashimada M, Zhuge F, Zhan L, Nagata N, Tsutsui A, et al. Astaxanthin prevents and reverses diet-induced insulin resistance and steatohepatitis in mice: A comparison with vitamin E. Sci Rep. 2015;5:17192 pubmed publisher
  • flow cytometry; mouse
Conde P, Rodriguez M, van der Touw W, Jimenez A, Burns M, Miller J, et al. DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance. Immunity. 2015;42:1143-58 pubmed publisher
  • flow cytometry; human
Sun C, Schattgen S, Pisitkun P, Jorgensen J, Hilterbrand A, Wang L, et al. Evasion of innate cytosolic DNA sensing by a gammaherpesvirus facilitates establishment of latent infection. J Immunol. 2015;194:1819-31 pubmed publisher
  • flow cytometry; mouse; fig s1
Evrard M, Chong S, Devi S, Chew W, Lee B, Poidinger M, et al. Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy. J Leukoc Biol. 2015;97:611-9 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4b
Jannasch K, Wegwitz F, Lenfert E, Maenz C, Deppert W, Alves F. Chemotherapy of WAP-T mouse mammary carcinomas aggravates tumor phenotype and enhances tumor cell dissemination. Int J Cancer. 2015;137:25-36 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Taniguchi T, Asano Y, Akamata K, Noda S, Takahashi T, Ichimura Y, et al. Fibrosis, vascular activation, and immune abnormalities resembling systemic sclerosis in bleomycin-treated Fli-1-haploinsufficient mice. Arthritis Rheumatol. 2015;67:517-26 pubmed publisher
  • immunohistochemistry - frozen section; mouse
  • flow cytometry; mouse
Liu G, Zhang W, Xiao Y, Lu P. Critical Role of IP-10 on Reducing Experimental Corneal Neovascularization. Curr Eye Res. 2015;40:891-901 pubmed publisher
  • flow cytometry; mouse
Döring M, Lessin I, Frenz T, Spanier J, Kessler A, Tegtmeyer P, et al. M27 expressed by cytomegalovirus counteracts effective type I interferon induction of myeloid cells but not of plasmacytoid dendritic cells. J Virol. 2014;88:13638-50 pubmed publisher
  • flow cytometry; mouse
Bernasconi E, D Angelo F, Michetti P, Velin D. Critical role of the GM-CSF signaling pathway in macrophage pro-repair activities. Pathobiology. 2014;81:183-9 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500
de Bock L, Somers K, Fraussen J, Hendriks J, van Horssen J, Rouwette M, et al. Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis. J Immunol. 2014;193:2147-56 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200
Watatani H, Maeshima Y, Hinamoto N, Yamasaki H, Ujike H, Tanabe K, et al. Vasohibin-1 deficiency enhances renal fibrosis and inflammation after unilateral ureteral obstruction. Physiol Rep. 2014;2: pubmed publisher
  • flow cytometry; mouse
Forgèt M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, et al. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer. PLoS ONE. 2014;9:e98623 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50
Orim F, Bychkov A, Shimamura M, Nakashima M, Ito M, Matsuse M, et al. Thyrotropin signaling confers more aggressive features with higher genomic instability on BRAF(V600E)-induced thyroid tumors in a mouse model. Thyroid. 2014;24:502-10 pubmed publisher
Phillips E, Lorch A, Durkes A, Goergen C. Early pathological characterization of murine dissecting abdominal aortic aneurysms. APL Bioeng. 2018;2:046106 pubmed publisher
Kortlever R, Sodir N, Wilson C, Burkhart D, Pellegrinet L, Brown Swigart L, et al. Myc Cooperates with Ras by Programming Inflammation and Immune Suppression. Cell. 2017;171:1301-1315.e14 pubmed publisher
Kennelly K, Holmes T, Wallace D, O Farrelly C, Keegan D. Early Subretinal Allograft Rejection Is Characterized by Innate Immune Activity. Cell Transplant. 2017;26:983-1000 pubmed publisher
Hudson N, Powner M, Sarker M, Burgoyne T, Campbell M, Ockrim Z, et al. Differential apicobasal VEGF signaling at vascular blood-neural barriers. Dev Cell. 2014;30:541-52 pubmed publisher
Khare R, Hillestad M, Xu Z, Byrnes A, Barry M. Circulating antibodies and macrophages as modulators of adenovirus pharmacology. J Virol. 2013;87:3678-86 pubmed publisher
Fisher J, McKenzie T, Lillegard J, Yu Y, Juskewitch J, Nedredal G, et al. Role of Kupffer cells and toll-like receptor 4 in acetaminophen-induced acute liver failure. J Surg Res. 2013;180:147-55 pubmed publisher
Pagani A, Nai A, Corna G, Bosurgi L, Rovere Querini P, Camaschella C, et al. Low hepcidin accounts for the proinflammatory status associated with iron deficiency. Blood. 2011;118:736-46 pubmed publisher
Khare R, May S, Vetrini F, Weaver E, Palmer D, Rosewell A, et al. Generation of a Kupffer cell-evading adenovirus for systemic and liver-directed gene transfer. Mol Ther. 2011;19:1254-62 pubmed publisher
Ibe S, Qin Z, Schuler T, Preiss S, Blankenstein T. Tumor rejection by disturbing tumor stroma cell interactions. J Exp Med. 2001;194:1549-59 pubmed
image
image 1 :
Bio-Rad MCA497EL image 1
Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 ( MCA497A647 ) at a 1/5 dilution and CD11b ( MCA74F ) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock ( BUF041B )
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA497EL
Description :
Rat anti Mouse F4/80:Low Endotoxin
Specificity :
F4/80
TargetSpecies :
Mouse
Host :
Rat
Format :
Low Endotoxin
Isotypes :
IgG2b
Applications :
F,WB,C,P,IP,R,FN,RE
ApplicationTypes :
Flow Cytometry,Western Blotting,Immunohistology - Frozen,Immunohistology - Paraffin,Immunoprecipitation,Radioimmunoassays,Functional Assays,Immunohistology - Resin
Clone :
Cl:A3-1
Quantity :
0.5 mg
Quantity Value :
0.5
Quantity Type :
mg
GO Accessions :
GO:0005509,GO:0016021,GO:0004930,GO:0007218,GO:0009897
GOTerms :
calcium ion binding,integral to membrane,G-protein coupled receptor activity,neuropeptide signaling pathway,external side of plasma membrane
Uniprot Accessions :
Q61549
Gene :
Emr1
GeneID URL :
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=13733
Entity Formats :
Alexa Fluor® 488,Alexa Fluor® 647,APC,Biotin,FITC,Low Endotoxin,Pacific Blue®,Purified,RPE,S/N,Alexa Fluor® 700,StarBright Blue 700,Amethyst Orange,StarBright Violet 440,StarBright Violet 515,StarBright Violet 610,StarBright Violet 670,StarBright Violet 570,StarBright Violet 710,StarBright Violet 790,StarBright Violet 475,StarBright UltraViolet 400,StarBright UltraViolet 665,StarBright UltraViolet 795,StarBright Violet 760,StarBright UltraViolet 445,StarBright UltraViolet 510,StarBright UltraViolet 740,StarBright UltraViolet 575,StarBright UltraViolet 605,StarBright Blue 580,StarBright Blue 615,StarBright Blue 675,StarBright Blue 765,StarBright Blue 810,StarBright Yellow 575,StarBright Yellow 605,StarBright Yellow 665,StarBright Yellow 800,StarBright Yellow 720,StarBright Red 670,StarBright Red 715,StarBright Red 775,StarBright Red 815
UNSPSC :
41116161
Concentration :
IgG concentration 1 mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
459
PriceEUR :
641
PriceUSD :
641 USD
PriceCHF :
697
PriceSEK :
7307
PriceNOK :
7297
PriceDKK :
4781
more info or order :
Bio-Rad product webpage
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.