Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .