Published customer image: . Mouse anti Rat MHC class II antibody, clone OX-3 ( MCA45R ) used to label MHC class II expressing cells in formalin fixed mouse brain cryosections by immunohistochemistry. Image caption: . (a) Representative immunostained micrographs show Iba1 + cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10 um. Quantitative results of Iba1 + cell areas (b) and the number of Iba1 + cells (c) in the SN of female and male mice at different ages (n = 5). *(p 0.05), **(p 0.01), ***(p 0.001): versus the opposite sex. (d) Representative immunostained micrographs show MHC class II + cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10 um. Quantitative results of MHC class II + cell areas (e) and the number of MHC class II + cells (f) in the SN of female and male mice at different ages (n = 5). ***(p 0.001): versus the opposite sex. (g) Representative immunostained micrographs show TH + cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 200 um. (h) Quantitative results of TH+ cells in the SN of female and male mice at different ages (n = 5). *(p 0.05), **(p 0.01): versus the opposite sex. From: Wu SY, Chen YW, Tsai SF, Wu SN, Shih YH, Jiang-Shieh YF, Yang TT, Kuo YM. Estrogen ameliorates microglial activation by inhibiting the Kir2.1 inward-rectifier K(+) channel. Sci Rep. 2016 Mar 10;6:22864.