product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti MBP (aa82-87)
catalog :
MCA409S
quantity :
2 ml
price :
508 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
12
reactivity :
human, mouse, rat, bovine, pigs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
Bio-Rad product webpage
citations: 46
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1b
Bauch J, Faissner A. The Extracellular Matrix Proteins Tenascin-C and Tenascin-R Retard Oligodendrocyte Precursor Maturation and Myelin Regeneration in a Cuprizone-Induced Long-Term Demyelination Animal Model. Cells. 2022;11: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 6a
Bauch J, Ort S, Ulc A, Faissner A. Tenascins Interfere With Remyelination in an Ex Vivo Cerebellar Explant Model of Demyelination. Front Cell Dev Biol. 2022;10:819967 pubmed publisher
  • immunocytochemistry; rat; 1:500; fig 2i
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1w, s1k
Aberle T, Piefke S, Hillg xe4 rtner S, Tamm E, Wegner M, K xfc spert M. Transcription factor Zfp276 drives oligodendroglial differentiation and myelination by switching off the progenitor cell program. Nucleic Acids Res. 2022;50:1951-1968 pubmed publisher
  • immunocytochemistry; rat; 1:500; loading ...; fig e6c
Dorrier C, Aran D, Haenelt E, Sheehy R, Hoi K, Pintarić L, et al. CNS fibroblasts form a fibrotic scar in response to immune cell infiltration. Nat Neurosci. 2021;24:234-244 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig e5m
Paredes I, Vieira J, Shah B, Ramunno C, Dyckow J, Adler H, et al. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk. Nat Neurosci. 2021;24:478-488 pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig 7a
Wiemann S, Reinhard J, Reinehr S, Cibir Z, Joachim S, Faissner A. Loss of the Extracellular Matrix Molecule Tenascin-C Leads to Absence of Reactive Gliosis and Promotes Anti-inflammatory Cytokine Expression in an Autoimmune Glaucoma Mouse Model. Front Immunol. 2020;11:566279 pubmed publisher
  • western blot; mouse; 1:300; loading ...; fig 3h, 3i, 3l, 3m
Wüst H, Wegener A, Fröb F, Hartwig A, Wegwitz F, Kari V, et al. Egr2-guided histone H2B monoubiquitination is required for peripheral nervous system myelination. Nucleic Acids Res. 2020;48:8959-8976 pubmed publisher
  • immunohistochemistry; rat; 1:250; loading ...; fig 4g
Werkman I, Sikkema A, Versluijs J, Qin J, de Boer P, Baron W. TLR3 agonists induce fibronectin aggregation by activated astrocytes: a role of pro-inflammatory cytokines and fibronectin splice variants. Sci Rep. 2020;10:532 pubmed publisher
  • western blot; mouse; 1:10,000; loading ...; fig 7c
Schäfer I, Kaisler J, Scheller A, Kirchhoff F, Haghikia A, Faissner A. Conditional Deletion of LRP1 Leads to Progressive Loss of Recombined NG2-Expressing Oligodendrocyte Precursor Cells in a Novel Mouse Model. Cells. 2019;8: pubmed publisher
  • immunocytochemistry; rat; 1:500; loading ...; fig 1a
Neumann B, Baror R, Zhao C, SEGEL M, Dietmann S, Rawji K, et al. Metformin Restores CNS Remyelination Capacity by Rejuvenating Aged Stem Cells. Cell Stem Cell. 2019;25:473-485.e8 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 2a
SEGEL M, Neumann B, Hill M, Weber I, Viscomi C, Zhao C, et al. Niche stiffness underlies the ageing of central nervous system progenitor cells. Nature. 2019;573:130-134 pubmed publisher
  • immunohistochemistry; pigs ; 1:200; loading ...; fig s11a
Vrselja Z, Daniele S, Silbereis J, Talpo F, Morozov Y, Sousa A, et al. Restoration of brain circulation and cellular functions hours post-mortem. Nature. 2019;568:336-343 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 2c
Gerber D, Ghidinelli M, Tinelli E, Somandin C, Gerber J, Pereira J, et al. Schwann cells, but not Oligodendrocytes, Depend Strictly on Dynamin 2 Function. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1d
Montani L, Pereira J, Norrmen C, Pohl H, Tinelli E, Trötzmüller M, et al. De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination. J Cell Biol. 2018;217:1353-1368 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 5b
Sousa A, Zhu Y, Raghanti M, Kitchen R, Onorati M, Tebbenkamp A, et al. Molecular and cellular reorganization of neural circuits in the human lineage. Science. 2017;358:1027-1032 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1e
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3a
Medina Rodríguez E, Bribian A, Boyd A, Palomo V, Pastor J, Lagares A, et al. Promoting in vivo remyelination with small molecules: a neuroreparative pharmacological treatment for Multiple Sclerosis. Sci Rep. 2017;7:43545 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1c
Berghoff S, Gerndt N, Winchenbach J, Stumpf S, Hosang L, Odoardi F, et al. Dietary cholesterol promotes repair of demyelinated lesions in the adult brain. Nat Commun. 2017;8:14241 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 7f
Kemp K, Cerminara N, Hares K, Redondo J, Cook A, Haynes H, et al. Cytokine therapy-mediated neuroprotection in a Friedreich's ataxia mouse model. Ann Neurol. 2017;81:212-226 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; tbl 1
Bassett E, Tokarew N, Allemano E, Mazerolle C, Morin K, Mears A, et al. Norrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation. elife. 2016;5: pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500; fig s2f
Figueres Oñate M, López Mascaraque L. Adult Olfactory Bulb Interneuron Phenotypes Identified by Targeting Embryonic and Postnatal Neural Progenitors. Front Neurosci. 2016;10:194 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3
  • western blot; mouse; 1:1000; fig 2
Gökbuget D, Pereira J, Bachofner S, Marchais A, Ciaudo C, Stoffel M, et al. The Lin28/let-7 axis is critical for myelination in the peripheral nervous system. Nat Commun. 2015;6:8584 pubmed publisher
  • western blot; human; 1:500
Henstridge C, Jackson R, Kim J, Herrmann A, Wright A, Harris S, et al. Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse. Acta Neuropathol Commun. 2015;3:53 pubmed publisher
  • immunocytochemistry; rat; 1:50; fig 2
  • western blot; rat; 1:500; fig 2
  • western blot; mouse; 1:500; fig 1
Müller C, Hochhaus N, Fontana X, Luhmann H, White R. SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis. PLoS ONE. 2015;10:e0136900 pubmed publisher
  • immunocytochemistry; rat; 1:500; fig 1
  • western blot; rat; 1:500; fig 1
Ettle B, Reiprich S, Deusser J, Schlachetzki J, Xiang W, Prots I, et al. Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells. Mol Cell Neurosci. 2014;62:68-78 pubmed publisher
  • immunohistochemistry - paraffin section; pigs ; 1:50
Zhou X, Xie Q, Xi G, Keep R, Hua Y. Brain CD47 expression in a swine model of intracerebral hemorrhage. Brain Res. 2014;1574:70-6 pubmed publisher
  • western blot; mouse; 1:200
May V, Ettle B, Poehler A, Nuber S, Ubhi K, Rockenstein E, et al. ?-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy. Neurobiol Aging. 2014;35:2357-68 pubmed publisher
Mészáros L, Riemenschneider M, Gassner H, Marxreiter F, von Horsten S, Hoffmann A, et al. Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophy. Acta Neuropathol Commun. 2021;9:68 pubmed publisher
Vaquié A, Sauvain A, Duman M, Nocera G, Egger B, Meyenhofer F, et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep. 2019;27:3152-3166.e7 pubmed publisher
Manso C, Querol L, Lleixà C, Poncelet M, Mekaouche M, Vallat J, et al. Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo. J Clin Invest. 2019;129:2222-2236 pubmed publisher
Spitzer S, Sitnikov S, Kamen Y, Evans K, Kronenberg Versteeg D, Dietmann S, et al. Oligodendrocyte Progenitor Cells Become Regionally Diverse and Heterogeneous with Age. Neuron. 2019;101:459-471.e5 pubmed publisher
Weider M, Starost L, Groll K, Küspert M, Sock E, Wedel M, et al. Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning. Nat Commun. 2018;9:899 pubmed publisher
Dillenburg A, Ireland G, Holloway R, Davies C, Evans F, Swire M, et al. Activin receptors regulate the oligodendrocyte lineage in health and disease. Acta Neuropathol. 2018;135:887-906 pubmed publisher
Xiao L, Ohayon D, McKenzie I, Sinclair Wilson A, Wright J, Fudge A, et al. Rapid production of new oligodendrocytes is required in the earliest stages of motor-skill learning. Nat Neurosci. 2016;19:1210-1217 pubmed publisher
Porcu G, Serone E, De Nardis V, Di Giandomenico D, Lucisano G, Scardapane M, et al. Clobetasol and Halcinonide Act as Smoothened Agonists to Promote Myelin Gene Expression and RxRγ Receptor Activation. PLoS ONE. 2015;10:e0144550 pubmed publisher
Henstridge C, Jackson R, Kim J, Herrmann A, Wright A, Harris S, et al. Erratum to: Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse. Acta Neuropathol Commun. 2015;3:83 pubmed publisher
Rittchen S, Boyd A, Burns A, Park J, Fahmy T, Metcalfe S, et al. Myelin repair in vivo is increased by targeting oligodendrocyte precursor cells with nanoparticles encapsulating leukaemia inhibitory factor (LIF). Biomaterials. 2015;56:78-85 pubmed publisher
Donega M, Giusto E, Cossetti C, Schaeffer J, Pluchino S. Systemic injection of neural stem/progenitor cells in mice with chronic EAE. J Vis Exp. 2014;: pubmed publisher
Barateiro A, Domingues H, Fernandes A, Relvas J, Brites D. Rat cerebellar slice cultures exposed to bilirubin evidence reactive gliosis, excitotoxicity and impaired myelinogenesis that is prevented by AMPA and TNF-? inhibitors. Mol Neurobiol. 2014;49:424-39 pubmed publisher
Zoupi L, Markoullis K, Kleopa K, Karagogeos D. Alterations of juxtaparanodal domains in two rodent models of CNS demyelination. Glia. 2013;61:1236-49 pubmed publisher
Onishi S, Tatsumi Y, Wada K, Yang H, Sugiura Y, Setou M, et al. Sulfatide accumulation in the dystrophic terminals of gracile axonal dystrophy mice: lipid analysis using matrix-assisted laser desorption/ionization imaging mass spectrometry. Med Mol Morphol. 2013;46:160-5 pubmed publisher
Barateiro A, Miron V, Santos S, Relvas J, Fernandes A, Ffrench Constant C, et al. Unconjugated bilirubin restricts oligodendrocyte differentiation and axonal myelination. Mol Neurobiol. 2013;47:632-44 pubmed publisher
Walczak P, All A, Rumpal N, Gorelik M, Kim H, Maybhate A, et al. Human glial-restricted progenitors survive, proliferate, and preserve electrophysiological function in rats with focal inflammatory spinal cord demyelination. Glia. 2011;59:499-510 pubmed publisher
Gordon D, Scolding N. Human mesenchymal stem cell culture for neural transplantation. Methods Mol Biol. 2009;549:103-18 pubmed publisher
Walczak P, Kedziorek D, Gilad A, Barnett B, Bulte J. Applicability and limitations of MR tracking of neural stem cells with asymmetric cell division and rapid turnover: the case of the shiverer dysmyelinated mouse brain. Magn Reson Med. 2007;58:261-9 pubmed
Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, et al. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature. 2006;441:880-4 pubmed
Walczak P, Kedziorek D, Gilad A, Lin S, Bulte J. Instant MR labeling of stem cells using magnetoelectroporation. Magn Reson Med. 2005;54:769-74 pubmed
image
image 1 :
Bio-Rad MCA409S image 1
Published customer image: . Rat anti MBP antibody, clone 12 ( MCA409S ) used to identify rat oligodendrocyte progenitors in vitro by immunofluorescence. Image caption: . Protandim promotes differentiation of primary rat OPCs under oxidative stress. After 2 day de-differentiation of OLs with growth factors bFGF-2 and PDGF-AA, primary rat OPCs were incubated with 30 ug/mL Protandim or vehicle control (EtOH) for 24 h. After removal of medium, cells were subsequently exposed to either control medium or medium with 10 uM tert-butyl hydrogen peroxide (tbH 2 O 2 ) for 5 days. MBP and Olig2 expression were assayed by immunocytochemistry. Data are presented as percentage of control and expressed as the mean SEM of 3 independent experiments. Statistics reflect student s t-test, one-tailed; * p 0.05. From: Lim JL, van der Pol SM, Baron W, McCord JM, de Vries HE, van Horssen J. Protandim Protects Oligodendrocytes against an Oxidative Insult. . Antioxidants (Basel). 2016 Sep 7;5(3). pii: E30 . This is an open access article distributed under the terms of the Creative Commons Attribution License .
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
Region :
(aa82-87)
ProductCode :
MCA409S
Description :
Rat anti MBP (aa82-87)
Specificity :
MBP
TargetSpecies :
Bovine
Host :
Rat
Format :
S/N
Isotypes :
IgG2a
Applications :
WB,E,IF,R
ApplicationTypes :
Western Blotting,ELISA,Immunofluorescence,Radioimmunoassays
Cross Reactivities :
Mouse,Rabbit,Mammals,Rat,Guinea Pig,Sheep,Human,Chicken,Pig
Clone :
12
Quantity :
2 ml
Quantity Value :
2
Quantity Type :
ml
GO Accessions :
GO:0005886,GO:0005515,GO:0019911,GO:0043209
GOTerms :
plasma membrane,protein binding,structural constituent of myelin sheath,myelin sheath
Uniprot Accessions :
P02687
Gene :
MBP
GeneID URL :
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=618684
Published Synonyms :
Myelin Basic Protein
Entity Formats :
S/N
UNSPSC :
41116161
Product Form :
Tissue Culture Supernatant - liquid
PriceGBP :
363
PriceEUR :
508
PriceUSD :
508 USD
PriceCHF :
553
PriceSEK :
5791
PriceNOK :
5783
PriceDKK :
3789
NotForSaleInCountries :
AU
more info or order :
Bio-Rad product webpage
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.