Published customer image: . Mouse anti Influenza A Nucleoprotein antibody, clone AA5H used for the detection of viral nucleoproitein in influenza infected HEK-293 cells by western blotting. Image caption: . Forced expression of influenza viral proteins does not result in impaired IFN -induced STAT1 and STAT2 phosphorylation. HEK293 cells were transfected with 500 ng plasmid DNA for expression of viral NP, M, NS, (A) PA, PB1 and PB2 (C) genes (see Table 1 for accession numbers of viral genes) using L2000 according to manufacturer s instructions. Note that the Pol II constructs in use also give rise to expression of second reading frames in the NS, M and PB1 genes (NS2, M2, PB1-F2). 48 h post transfection cells were stimulated with human IFN (500 U/ml) for 15 minutes. Total protein lysates were subjected to Western blot analysis using anti-phospho-STAT1, anti-phospho-STAT2, anti-STAT1 antibodies. Expression of influenza viral proteins was monitored with antibodies against NP, M1, NS1, PA, PB1 or PB2. (E) HEK293 cells were infected with the human influenza virus PR8 (H1N1) (MOI = 5) for the indicated time points and were subsequently stimulated for 15 min with either human IFN at a concentration of 100 U/ml. Cell lysates were subjected to Western blots as described. (B, D, F) Quantification of relative pSTAT1 and pSTAT2 band intensities in A, C and E using AIDA software and 2D densitometry (Fuji). From: Pauli E-K, Schmolke M, Wolff T, Viemann D, Roth J, Bode JG, et al. (2008) Influenza A Virus Inhibits Type I IFN Signaling via NF- B-Dependent Induction of SOCS-3 Expression. PLoS Pathog 4(11): e1000196 .