Published customer image: . Mouse anti Human CD172 antibody, clone 15-414 ( MCA2518 ) used for the demonstration of SIRP expression on human thymus cells by immunofluorescence and flow cytometry. Image caption: . Thymic Sirp + DC-SIGN + cDCs can uptake antigens, which leak inside the IVRs. (A) Triple-color fluorescent image with the combination of Col IV (green), Ly-51 (blue), and Sirp (red) in mouse thymic tissue. Dashed lines indicate the boundary between cortex (C) and medulla (M). Scale bar, 100 um. (B) A double-color fluorescent immunostaining for DC-SIGN (green) and Sirp (red) in human thymic tissue. Dashed lines indicate the outer margin of thymic lobule. Scale bar, 100 um. (C) Thymic B220 − CD11c high Sirp − (Sirp − ) and B220 − CD11c high Sirp + (Sirp + ) cDCs were sorted (Sirp − purity, 97.8%; Sirp + purity, 97.1%) by using FACSAria (BD Biosciences), from thymic low-density cells isolated from 10 mice. Expression of DC-SIGN, SIGNR1, SIGNR2, SIGNR3, and SIGNR4 was determined by RT-PCR. GAPDH served as an internal positive control. RT-PCR analysis was repeated three times. (D) DC-SIGN expression on CD11c high Sirp − and CD11c high Sirp + cDCs. Gray-filled and red-open histograms indicate the results obtained using isotype control and specific mAbs for DC-SIGN, respectively. (E) Thymi were collected at the indicated time points after FITC-conjugated dextran was intravenously injected into WT mice. Triple-color fluorescent images with the combination of dextran (green), DAPI (blue), and Col IV (red) are shown here. Scale bar, 200 um. (F) Triple-color fluorescent images with the combination of dextran (green), Col IV (blue), and Sirp (red) at 6 hrs after dextran injection are shown here. The confocal images in the parenchyma nearby small vessel and inside the IVRs, and superposed 3-D image inside the IVRs are shown in the upper and lower left, and lower right panels, respectively. Scale bars, 50 um. (G) Uptake of OVA647 in low density cells at 4 hrs after injection. Percentage of CD11c (+) OVA647 (+) and CD11c (−) OVA647 (+) region are shown. Expression of Sirp molecule in region 1 (R1) and R2 are represented by unfilled and gray-filled histogram, respectively. (H) Uptake of OVA647 at 4 hrs after intravenous, subcutaneous, or intraperitoneal injection. Percentage of Sirp (+) OVA647 (+) region in thymic CD11c high DCs is shown in each panel. PBS was injected as a control. Representative results from three independent experiments are shown here. From: Baba T, Badr MES, Tomaru U, Ishizu A, Mukaida N (2012). Novel Process of Intrathymic Tumor-Immune Tolerance through CCR2-Mediated Recruitment of Sirp + Dendritic Cells: A Murine Model. PLoS ONE 7(7): e41154 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .