Published customer image: . Phycoerythrin conjugated Mouse anti Bovine TNF alpha antibody, clone CC327 used for the evaluation of TNF- expression by bovine PBMC following M. bovis infection. Image caption: . Identification of multifunctional IFN- , IL-2 and TNF- CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN- , IL-2 and TNF- determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN- , IL-2 or TNF- following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN- + and IFN- − CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle. From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194 .