Published customer image: . Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on human cells by immunofluorescence. Image caption: . Desmoglein-3 and flotillin-2 colocalise in human epidermis and human epithelial cell lines. Human skin cryosections, HaCaT and MCF10A cells were immunostained for Dsg3 (red) and flotillin-2 (green). In human epidermis, flotillin-2 is expressed throughout the epidermal layer and colocalises with Dsg3 in keratinocytes of the stratum basale and suprabasale. In HaCaT keratinocytes, flotillin-2 is localised at the cell membrane and in intracellular vesicular structures. Dsg3 is localised at the plasma membrane where it colocalises with flotillin-2. In MCF10A mammary epithelial cells, flotillin-2 and Dsg3 show a more profound colocalisation at the sites of cell-cell contact. The cell lines were grown on glass coverslips for at least 3 days. Scale bar: 20um. From: V llner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R. Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes. Sci Rep. 2016 Jun 27;6:28820 .