Published customer image: . Mouse anti 5-Methylcytidine antibody, clone 33D3 ( MCA2201 ) used for the detection of 5-meC in Zea mays root tip cells by immunofluorescence. . Image caption: . Reduction in 5-meC in TSA- and 5-AC-treated cells. (A) Immunostaining for 5-meC in representative maize root tip cells in interphase or mitosis after TSA or 5-AC treatment. The 'DAPI' panel shows DAPI-stained DNA images, the '5-meC' panel shows immunostained images and the 'Merge' panel shows a combination of blue and green signals. As the untreated control cells progressed into metaphase and early telophase, the amount of 5-meC within mitotic chromosomes increased compared with the amount in interphase cells. The DNA methylation level was reduced in each cell cycle phase after TSA and 5-AC treatments compared with control cells. Bar = 5 μm. (B) Histogram showing mean gray values of the immunostaining signals for 5-meC. The mean gray value of 5-meC signal after treatment with TSA or 5-AC is lower than that of the control. The mean gray values for 5-meC were derived from Additional file 1: Table S3. Error bars represent standard error of the mean. From: Yang F, Zhang L, Li J, Huang J, Wen R, Ma L, Zhou D, Li L. Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize. BMC Plant Biol. 2010 Aug 18;10:178 .