Published customer image: . Mouse anti Humaqn CD206 antibody, clone 15-2 used for the identification of M2 macrophages in culture by immunofluorescence. Image caption: . Surface receptor expression and phagocytic activity of differentiated cells. G-LPS-M s (A, C) and M-IL-4-M s (B, D) were fixed with 5% formalin, then blocked and stained with anti- MHC class II (A, B) or anti-CD206 (C, D) antibodies. Merged images of fluorescence and counterstaining with DAPI show the expression of MHC class II (A; white arrows) on G-LPS-M s and CD206 (D) on M-IL-4-M s. Uptake of fluorescent latex beads in G-LPS-M s (E) and M-IL-4-M s (F). The cells were incubated with fluorescently labeled opsonized latex beads for 90 minutes. After washing with phosphate buffer, cells were stained with trypan blue. The fluorescent image is overlaid on phase contrast image of cells. Bars represent 50 μum. The total fluorescence collectively derived from the internalized beads and cell surface-associated particles was quantified by measuring the fluorescent intensity using a Fluorescence Spectrometer (G). This assay was performed in quintuplicate and repeated two times. Statistical analysis was performed using the Student t-test. * P 0.00001. From: Yamane, K. and Leung, K.-P. (2016),. Rabbit M1 and M2 macrophages can be induced by human recombinant GM-CSF and M-CSF. FEBS Open Bio. Accepted Author Manuscript. doi:10.1002/2211-5463.12101 .