Published customer image: . Mouse anti Bovine CD172a antibody, clone CC149 ( MCA2041GA ) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry. Image caption: . BM of Csf1r-EGFP sheep contains a heterogeneous EGFP + population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFP lo and EGFP hi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFP lo and GFP hi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP + cells (combined EGFP lo and EGFP hi ). Scale bar, 10 um. Dot plots and Leishman staining of EGFP + cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal. From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep. J Immunol. 2016 Sep 15;197(6):2297-305 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .