Published customer image: . Mouse anti Human CD151 antibody, clone 11G5a ( MCA1856 ) used for the detection of CD151 positive microdomains on the surface and in intracellular vesicles in pseudovirus infected HeLa cells by immuneoelectron microscopy. Image caption: . Virions associate with CD151 positive microdomains on the cell surface and in intracellular vesicles. (A) HeLa cells were fixed with paraformaldehyde and the cell surface was immunostained with a monoclonal anti-CD63 antibody and polyclonal anti-CD151 antibody. (B) HeLa cells were exposed to HPV16 pseudovirions for the indicated time periods, and fixed with paraformaldehyde. The cell surface was immunostained with polyclonal anti-L1 antibody (K75, red) and a monoclonal anti-CD151 antibody (sc-5275). Inserts display enlarged sections that are shown in the right column. (C) HeLa cells were exposed to HPV16 pseudovirions for 7 hours. Cells were fixed and permeabilized with methanol. Intracellular PsVs were immunostained with monoclonal anti-L1 antibody (L1-7, red) and CD151 with a polyclonal anti-CD151 antibody (sc-33123, green). Bars, 20 um. (D) HeLa cells were infected with pseudovirions for 4 hours and then fixed with paraformaldehyde. Cell surface CD151 was immunolabeled with 10 nm gold particles. Pseudovirions and CD151 were visualized by electron microscopy. Bar, 200 nm. From: Spoden G, Freitag K, Husmann M, Boller K, Sapp M, Lambert C, et al. (2008). Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs). PLoS ONE 3(10): e3313 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .