product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Mouse anti Sheep Interleukin-8
catalog :
MCA1660
quantity :
0.25 mg
price :
411 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8M6
reactivity :
bovine, pigs , domestic sheep
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Bio-Rad product webpage
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; domestic sheep; 1:4000; loading ...; fig 6b
Melville J, McDonald C, Bischof R, Polglase G, Lim R, Wallace E, et al. Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs. PLoS ONE. 2017;12:e0173572 pubmed publisher
  • immunohistochemistry - paraffin section; pigs ; 1:1000; tbl 1
Erneholm K, Lorenzen E, Bøje S, Olsen A, Andersen P, Cassidy J, et al. Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D. BMC Vet Res. 2016;12:200 pubmed publisher
  • ELISA; bovine; fig 3a
Tahoun A, Jensen K, Corripio Miyar Y, McAteer S, Corbishley A, Mahajan A, et al. Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella. Vet Res. 2015;46:9 pubmed publisher
Schirmer L, Atallah P, Freudenberg U, Werner C. Chemokine-Capturing Wound Contact Layer Rescues Dermal Healing. Adv Sci (Weinh). 2021;8:e2100293 pubmed publisher
Redondo E, Gazquez A, Vadillo S, Garcia A, Franco A, Masot A. Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus. J Comp Pathol. 2014;150:434-48 pubmed publisher
Laursen H, Jensen H, Leifsson P, Jensen L, Christiansen J, Trebbien R, et al. Immunohistochemical detection of interleukin-8 in inflamed porcine tissues. Vet Immunol Immunopathol. 2014;159:97-102 pubmed publisher
Stinson L, Ireland D, Kemp M, Payne M, Stock S, Newnham J, et al. Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation in ex vivo human and ovine fetal membranes. Reproduction. 2014;147:313-20 pubmed publisher
Schnabel C, Wagner S, Wagner B, Duran M, Babasyan S, Nolte I, et al. Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines. Vet Immunol Immunopathol. 2013;156:1-19 pubmed publisher
Christiansen J, Jensen H, Jensen L, Koch J, Aalbaek B, Nielsen O, et al. Systemic inflammatory response and local cytokine expression in porcine models of endocarditis. APMIS. 2014;122:292-300 pubmed publisher
Zhang L, Saito M, Jobe A, Kallapur S, Newnham J, Cox T, et al. Intra-amniotic administration of E coli lipopolysaccharides causes sustained inflammation of the fetal skin in sheep. Reprod Sci. 2012;19:1181-9 pubmed publisher
Soerensen K, Skovgaard K, Heegaard P, Jensen H, Nielsen O, Leifsson P, et al. The impact of Staphylococcus aureus concentration on the development of pulmonary lesions and cytokine expression after intravenous inoculation of pigs. Vet Pathol. 2012;49:950-62 pubmed publisher
Bonnefont C, Rainard P, Cunha P, Gilbert F, Toufeer M, Aurel M, et al. Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant. Physiol Genomics. 2012;44:403-16 pubmed publisher
image
image 1 :
Bio-Rad MCA1660 image 1
Published customer image: . Mouse antiSheep interleukin-8 antbody, clone 8M6 (MCA1660) used, in conjunction with Rabbit anti Sheep interleuin-8 antibody ( AHP425 ) for the measurement of ovine CXCL8 in embryonal bovine lungs by ELISA. Image caption: . Analysis of bovine TLR5 activity in bovine epithelial cells. . The bovine EBL cell line was stably transfected with a bTLR5 clone and CXCL8 secretion assayed following challenge with flagellin. (A) CXCL8 levels from EBLs with and without transfection of bTLR5. H7 flagellin at a range of concentrations was added to the cells and CXCL8 levels were measured by ELISA following overnight incubation. Transfection with bTLR5 resulted in significantly higher levels of CXCL8 being produced by the bTLR5+ cells with addition of 50-50,000 ng/mL of H7 flagellin (p   0.001). The data shown are the means and 95% confidence intervals. (B) Analysis of CXCL8 production from EBL-TLR5 in response to addition of native H7 or an H7 flagellin preparation from which the majority of LPS has been removed. Medians and interquartile ranges are shown. (C) Secreted CXCL8 following addition of WT and mutated H7 flagellins to EBLs with transfected bTLR5. 50 ng/mL of WT-H7 and mutated flagellins were added to the EBLs transfected with bTLR5 and incubated overnight. CXCL8 was measured by ELISA. Addition of the R90T, R90A and L500A/I504A variants led to significantly lower levels of cytokine release (p  0.001) relative to WT-H7 stimulation (asterisks). R90T showed a significantly lower induction than R90A (p  0.001). Medians and interquartile ranges are shown. (D) Secreted CXCL8 following addition of WT H7 and the R90T flagellin mutant to EBL cells. A range of flagellin concentrations was incubated overnight with the cells and CXCL8 measured by ELISA. R90T flagellin demonstrated significantly reduced levels of CXCL8 activation at 50 ng/mL and 500 ng/mL (p  0.001), marked by asterisks. The data shown are the means and 95% confidence intervals. All CXCL8 data shown is from a minimum of three biological replicates. From: Tahoun A, et al. . Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella. Vet Res. 2015 Feb 19;46:9 .
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA1660
Description :
Mouse anti Sheep Interleukin-8
Specificity :
IL-8
TargetSpecies :
Sheep
Host :
Mouse
Format :
Purified
Isotypes :
IgG2a
Applications :
WB,F,E,FN
ApplicationTypes :
Western Blotting,Flow Cytometry,ELISA,Functional Assays
Cross Reactivities :
Dog,Rabbit,Bovine,Pig,Mustelid,Ferret,Mink,Cat
Clone :
8M6
Quantity :
0.25 mg
Quantity Value :
0.25
Quantity Type :
mg
GO Accessions :
GO:0005153,GO:0005615,GO:0006954,GO:0006955,GO:0007050,GO:0008009,GO:0030155,GO:0030593,GO:0045091,GO:0050930
GOTerms :
interleukin-8 receptor binding,extracellular space,inflammatory response,immune response,cell cycle arrest,chemokine activity,regulation of cell adhesion,neutrophil chemotaxis,regulation of retroviral genome replication,induction of positive chemotaxis
Uniprot Accessions :
P36925
Gene :
IL8
GeneID URL :
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=443418
Published Synonyms :
CXCL8
Entity Formats :
Purified
UNSPSC :
41116161
Concentration :
IgG concentration 1.0 mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
294
PriceEUR :
411
PriceUSD :
411 USD
PriceCHF :
447
PriceSEK :
4480
PriceNOK :
4502
PriceDKK :
3064
more info or order :
Bio-Rad product webpage
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.