Published customer image: . Mouse anti Bovine CD205 antibody, clone CC98 ( MCA1651GA ) used to evaluate CD205 expression on ovine lymph derived dendritic cells by flow cytometry. Image caption: . CD1b+ L-DCs are migratory mature DC capable of endocytic and phagocytic activities. After labelling lymph cells with anti- CD1b mAb (A), CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b+ L-DCs are shown (C). May-Gr nwald Giemsa staining of sorted and cytocentrifugated CD1b+ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b+ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP- Salmonella by CD1b+ L- DCs. Lymph cells (1×10 6 cells) were incubated with FITC-OVA for 1h at 4 C or 37 C, or with GFP- Salmonella for 30 min at 37 C, followed by CD1b labelling. CD1b+ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b+ and CD1b- subsets and GFP- Salmonella fluorescence shown on dot plot H. From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012). Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells. PLoS ONE 7(1): e30430 .