Published customer image: . Rat anti Mouse IgG2a antibody, clone LO-MGSa-9 antibody ( MCA1588P ) used as a tertiary detection antibody for the detection of bound mouse IgG2a antibody in an antibody response ELISA following BSRV challenge. Image caption: . Mucosal IgA antibodies in the upper and lower airways, before and after BRSV challenge. Four groups of 5 calves were vaccinated as described in Fig. 1 and challenged with BRSV, 5 weeks after vaccination. Two weeks before challenge, one calf (c5) was euthanized due to traumatic injury. BRSV-specific IgA antibodies were analyzed by ELISA. (A) shows group mean levels of BRSV-specific IgA in nasal secretions on post-infection day (PID) 0 and 7, whereas (B) and (C) show group mean titers of total BRSV- and HRSV-N-specific IgA in bronchoalveolar lavage (BAL) on PID 7, respectively. BAL samples were titrated, whereas antibody levels in nasal secretions were semi-quantitatively determined and expressed as a percentage of a positive control sample, due to lack of sample material. Standard deviations are indicated by upward deflecting lines. Statistically significant differences between PID 0 and PID 7 in panel A are indicated by a horizontal line, whereas in all panels significant differences between groups for the same time-point are indicated by asterisks and the corresponding group letter; p 0.05 (*); p 0.01 (**); p 0.005 (***); p 0.001 (****). From: Blod rn K, H gglund S, Fix J, Dubuquoy C, Makabi-Panzu B, Thom M, et al. (2014). Vaccine Safety and Efficacy Evaluation of a Recombinant Bovine Respiratory Syncytial Virus (BRSV) with Deletion of the SH Gene and Subunit Vaccines Based On Recombinant Human RSV Proteins: N-nanorings, P and M2-1, in Calves with Maternal Antibodies. PLoS ONE 9(6): e100392 .