product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti Human TNF Alpha
catalog :
MCA1560
quantity :
0.5 mg
price :
462 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MP9-20A4
reactivity :
human
application :
western blot, ELISA, flow cytometry, blocking or activating experiments
more info or order :
citations: 2
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; loading ...; fig 5b
Poussin C, Gallitz I, Schlage W, Steffen Y, Stolle K, Lebrun S, et al. Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis. Toxicol In Vitro. 2014;28:896-908 pubmed publisher
Kumagai M, Shimoda S, Wakabayashi R, Kunisawa Y, Ishii T, Osada K, et al. Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination. J Control Release. 2012;160:542-51 pubmed publisher
image
image 1 :
Bio-Rad MCA1560 image 1
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat IgG1 ( MCA6004PE ). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA1560
Description :
Rat anti Human TNF Alpha
Specificity :
TNF Alpha
TargetSpecies :
Human
Host :
Rat
Format :
Purified
Isotypes :
IgG1
Applications :
E,F,C,WB
ApplicationTypes :
ELISA,Flow Cytometry,Immunohistology - Frozen,Western Blotting
Clone :
MP9-20A4
Quantity :
0.5 mg
Quantity Value :
0.5
Quantity Type :
mg
GO Accessions :
GO:0000060,GO:0000185,GO:0000187,GO:0001891,GO:0002439,GO:0002740,GO:0009615,GO:0005887,GO:0005125,GO:0005164,GO:0005615,GO:0006916,GO:0006919,GO:0006927,GO:0050796,GO:0042802,GO:0009651,GO:0009897,GO:0010843,GO:0010888,GO:0016563,GO:0016564,GO:0030730,GO:0031334,GO:0031622,GO:0032582,GO:0032715,GO:0032722,GO:0032800,GO:0033138,GO:0033209,GO:0034116,GO:0042346,GO:0043123,GO:0043193,GO:0043243,GO:0045071,GO:0045080,GO:0045121,GO:0045429,GO:0045599,GO:0045672,GO:0048661,GO:0050715,GO:0050901,GO:0050995,GO:0051044,GO:0051092,GO:0051384,GO:0055037,GO:0060555,GO:0060557,GO:0060559,GO:0061048,GO:0070265,GO:0070374
GOTerms :
protein import into nucleus, translocation,activation of MAPKKK activity,activation of MAPK activity,phagocytic cup,chronic inflammatory response to antigenic stimulus,negative regulation of cytokine secretion involved in immune response,response to virus,integral to plasma membrane,cytokine activity,tumor necrosis factor receptor binding,extracellular space,anti-apoptosis,activation of caspase activity,transformed cell apoptosis,regulation of insulin secretion,identical protein binding,response to salt stress,external side of plasma membrane,promoter binding,negative regulation of lipid storage,transcription activator activity,transcription repressor activity,sequestering of triglyceride,positive regulation of protein complex assembly,positive regulation of fever generation,negative regulation of gene-specific transcription,negative regulation of interleukin-6 production,positive regulation of chemokine production,receptor biosynthetic process,positive regulation of peptidyl-serine phosphorylation,tumor necrosis factor-mediated signaling pathway,positive regulation of heterotypic cell-cell adhesion,positive regulation of NF-kappaB import into nucleus,positive regulation of I-kappaB kinase/NF-kappaB cascade,positive regulation of gene-specific transcription,positive regulation of protein complex disassembly,negative regulation of viral genome replication,positive regulation of chemokine biosynthetic process,membrane raft,positive regulation of nitric oxide biosynthetic process,negative regulation of fat cell differentiation,positive regulation of osteoclast differentiation,positive regulation of smooth muscle cell proliferation,positive regulation of cytokine secretion,leukocyte tethering or rolling,negative regulation of lipid catabolic process,positive regulation of membrane protein ectodomain proteolysis,positive regulation of NF-kappaB transcription factor activity,response to glucocorticoid stimulus,recycling endosome,induction of necroptosis by extracellular signals,positive regulation of vitamin D biosynthetic process,positive regulation of calcidiol 1-monooxygenase activity,negative regulation of branching involved in lung morphogenesis,necrotic cell death,positive regulation of ERK1 and ERK2 cascade
Uniprot Accessions :
P01375
Gene :
TNF
GeneID URL :
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7124
Entity Formats :
Alexa Fluor® 488,Alexa Fluor® 647,Purified
UNSPSC :
41116161
Concentration :
1.0 mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
332
PriceEUR :
462
PriceUSD :
462 USD
PriceCHF :
503
PriceSEK :
5036
PriceNOK :
5061
PriceDKK :
3445
more info or order :
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.