product summary
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company name :
Bio-Rad
other brands :
Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology
product type :
antibody
product name :
Rat anti Human CD3
catalog :
MCA1477T
quantity :
25 µg
price :
103 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
CD3-12
reactivity :
African green monkey, human, mouse, dogs, cat
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Bio-Rad product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:600; loading ...; fig 4c
Zhang Q, Hresko M, Picton L, Su L, Hollander M, Nunez Cruz S, et al. A human orthogonal IL-2 and IL-2Rβ system enhances CAR T cell expansion and antitumor activity in a murine model of leukemia. Sci Transl Med. 2021;13:eabg6986 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3c
Guo X, Kolpakov M, Hooshdaran B, Schappell W, Wang T, Eguchi S, et al. Cardiac Expression of Factor X Mediates Cardiac Hypertrophy and Fibrosis in Pressure Overload. JACC Basic Transl Sci. 2020;5:69-83 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig e8d
Aghajanian H, Kimura T, Rurik J, Hancock A, Leibowitz M, Li L, et al. Targeting cardiac fibrosis with engineered T cells. Nature. 2019;573:430-433 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
De S, Li D, Song S, Matta B, Barnes B. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep. 2016;6:31002 pubmed publisher
  • immunocytochemistry; human; fig 3
Heylmann D, Kaina B. The γH2AX DNA damage assay from a drop of blood. Sci Rep. 2016;6:22682 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 6
Finkin S, Yuan D, Stein I, Taniguchi K, Weber A, Unger K, et al. Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma. Nat Immunol. 2015;16:1235-44 pubmed publisher
  • immunohistochemistry; human
de Winde C, Zuidscherwoude M, Vasaturo A, van der Schaaf A, Figdor C, van Spriel A. Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs. Histochem Cell Biol. 2015;144:133-46 pubmed publisher
  • immunohistochemistry - paraffin section; African green monkey
Kwun J, Page E, Hong J, GIBBY A, Yoon J, Farris A, et al. Neutralizing BAFF/APRIL with atacicept prevents early DSA formation and AMR development in T cell depletion induced nonhuman primate AMR model. Am J Transplant. 2015;15:815-22 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2
Kobayashi T, Hamaguchi Y, Hasegawa M, Fujimoto M, Takehara K, Matsushita T. B cells promote tumor immunity against B16F10 melanoma. Am J Pathol. 2014;184:3120-9 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
de Bock L, Somers K, Fraussen J, Hendriks J, van Horssen J, Rouwette M, et al. Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis. J Immunol. 2014;193:2147-56 pubmed publisher
  • immunohistochemistry; dogs; 1:500
Little J, Bleedorn J, Sutherland B, Sullivan R, Kalscheur V, Ramaker M, et al. Arthroscopic assessment of stifle synovitis in dogs with cranial cruciate ligament rupture. PLoS ONE. 2014;9:e97329 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Chan I, Jain R, Tessmer M, Gorman D, Mangadu R, Sathe M, et al. Interleukin-23 is sufficient to induce rapid de novo gut tumorigenesis, independent of carcinogens, through activation of innate lymphoid cells. Mucosal Immunol. 2014;7:842-56 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Puentes F, van der Star B, Victor M, Kipp M, Beyer C, Peferoen Baert R, et al. Characterization of immune response to neurofilament light in experimental autoimmune encephalomyelitis. J Neuroinflammation. 2013;10:118 pubmed publisher
  • immunocytochemistry; cat
Gilroy C, Forzán M, Drew A, Vernau W. Eosinophilia in a cat with acute leukemia. Can Vet J. 2011;52:1004-8 pubmed
Koppejan H, Jansen D, Hameetman M, Thomas R, Toes R, van Gaalen F. Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Res Ther. 2019;21:3 pubmed publisher
Magi G, Mariotti F, Berardi S, Piccinini A, Vullo C, Palumbo Piccionello A, et al. Loss of alpha-smooth muscle actin expression associated with chronic intestinal pseudo-obstruction in a young Miniature Bull Terrier. Acta Vet Scand. 2018;60:25 pubmed publisher
Haran K, Hajduczki A, Pampusch M, Mwakalundwa G, Vargas Inchaustegui D, Rakasz E, et al. Simian Immunodeficiency Virus (SIV)-Specific Chimeric Antigen Receptor-T Cells Engineered to Target B Cell Follicles and Suppress SIV Replication. Front Immunol. 2018;9:492 pubmed publisher
Janova H, Arinrad S, Balmuth E, Mitjans M, Hertel J, Habes M, et al. Microglia ablation alleviates myelin-associated catatonic signs in mice. J Clin Invest. 2018;128:734-745 pubmed publisher
Galler A, Rütgen B, Haas E, Saalmuller A, Hirt R, Gerner W, et al. Immunophenotype of Peripheral Blood Lymphocytes in Dogs with Inflammatory Bowel Disease. J Vet Intern Med. 2017;31:1730-1739 pubmed publisher
Silveira E, Rogers K, Gumber S, Amancha P, Xiao P, Woollard S, et al. Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus. J Immunol. 2017;199:1003-1011 pubmed publisher
Pfaender S, Walter S, Grabski E, Todt D, Bruening J, Romero Brey I, et al. Immune protection against reinfection with nonprimate hepacivirus. Proc Natl Acad Sci U S A. 2017;114:E2430-E2439 pubmed publisher
Lukas D, Yogev N, Kel J, Regen T, Mufazalov I, Tang Y, et al. TGF-β inhibitor Smad7 regulates dendritic cell-induced autoimmunity. Proc Natl Acad Sci U S A. 2017;114:E1480-E1489 pubmed publisher
Sieber M, Jaenisch N, Brehm M, Guenther M, Linnartz Gerlach B, Neumann H, et al. Attenuated inflammatory response in triggering receptor expressed on myeloid cells 2 (TREM2) knock-out mice following stroke. PLoS ONE. 2013;8:e52982 pubmed publisher
Ludes Meyers J, Kil H, Nunez M, Conti C, Parker Thornburg J, Bedford M, et al. WWOX hypomorphic mice display a higher incidence of B-cell lymphomas and develop testicular atrophy. Genes Chromosomes Cancer. 2007;46:1129-36 pubmed
image
image 1 :
Bio-Rad MCA1477T image 1
Figure A. RPE conjugated Mouse anti Human CD8 ( MCA1226PE ) and Alexa Fluor 647 conjugated Rat IgG1 isotype control ( MCA6004A647 ). Figure B. RPE conjugated Mouse anti Human CD8 ( MCA1226PE ) and Alexa Fluor 647 conjugated Rat anti Human CD3 ( MCA1477A647 ). All experiments performed on red cell lysed human peripheral blood gated on leukocytes in the presence of human SeroBlock ( BUF070A ).
product information
Entity Type :
Monoclonal Antibody
Entity Category :
Antibodies
ProductCode :
MCA1477T
Description :
Rat anti Human CD3
Specificity :
CD3
TargetSpecies :
Human
Host :
Rat
Format :
Purified
Isotypes :
IgG1
Applications :
P,F,C,IF
ApplicationTypes :
Immunohistology - Paraffin,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence
Cross Reactivities :
Bovine,Dog,Horse,Rhesus Monkey,Pig,Chicken,Mouse,Duck,Koala,Harbour Porpoise,Alpaca,Cynomolgus monkey,Spotted Hyena,Sea Lion,Cat,Amazon Parrot,Mammals,Birds,Raccoon,Great horned owl (Bubo virginianus),Bullfrog,Xenopus,Amphibia,Rabbit,African green monkey
Clone :
CD3-12
Quantity :
25 µg
Quantity Value :
25
Quantity Type :
µg
GO Accessions :
GO:0004888,GO:0005057,GO:0005887,GO:0007172,GO:0007186,GO:0009897,GO:0017124,GO:0019901,GO:0030159,GO:0031295,GO:0042608,GO:0046982,GO:0050852
GOTerms :
transmembrane receptor activity,receptor signaling protein activity,integral to plasma membrane,signal complex assembly,G-protein coupled receptor protein signaling pathway,external side of plasma membrane,SH3 domain binding,protein kinase binding,receptor signaling complex scaffold activity,T cell costimulation,T cell receptor binding,protein heterodimerization activity,T cell receptor signaling pathway
Uniprot Accessions :
P07766
Gene :
CD3E
GeneID URL :
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=916
Entity Formats :
Alexa Fluor® 488,Alexa Fluor® 647,FITC,Pacific Blue®,Purified
UNSPSC :
41116161
Concentration :
IgG concentration 1.0 mg/ml
Product Form :
Purified IgG - liquid
PriceGBP :
74
PriceEUR :
103
PriceUSD :
103 USD
PriceCHF :
112
PriceSEK :
1174
PriceNOK :
1172
PriceDKK :
768
more info or order :
Bio-Rad product webpage
company information
Bio-Rad
Endeavour House, Langford Business Park
Langford Lane, Kidlington
OXON, OX5 1GE
antibody_sales_uk@bio-rad.com
https://www.bio-rad-antibodies.com
1 800 265 7376 (North America)
44 (0)1865 852 700 (Rest of World)
headquarters: UK
Bio-Rad is one of the world's leading antibody manufacturers, offering over 12,000 antibodies and related reagents for a focused range of research areas such as immunology, cancer, veterinary research and cell biology through its antibody experts formerly known as AbD Serotec.
The range includes apoptosis kits, autophagy reagents, CD markers, epitope tag antibodies, immunology antibodies, neuroscience antibodies, and veterinary reagents.
Bio-Rad has an ISO 9001 and ISO 13485 certified production facility in the United Kingdom as well as ISO 9001 certified facilities in Germany and the United States.
Our expertise extends to traditional and recombinant antibody generation and production services. Our custom generation service delivers antibodies in as little as 8 weeks with greater than 90% success. The in vitro technology enables selection of antibodies to challenging targets, long term consistent supply, and provision of the antibody sequence.
At Bio-Rad we are committed to your success when using our antibodies and we focus on providing the technology, products and all the supporting information you need to excel in your research.