Rat anti Mouse CD45 | Bio-Rad MCA1388 product information

product summary

company name :

Bio-Rad

other brands :

Biogenesis, Serotec, AbD Serotec, Oxford Biotechnology

product type :

antibody

product name :

Rat anti Mouse CD45

catalog :

MCA1388

quantity :

0.25 mg

price :

372 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

IBL-3/16

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

Bio-Rad product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3a	Une H, Yamasaki R, Nagata S, Yamaguchi H, Nakamuta Y, Indiasari U, et al. Brain gray matter astroglia-specific connexin 43 ablation attenuates spinal cord inflammatory demyelination. J Neuroinflammation. 2021;18:126 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig 5s1e	Ma W, Silverman S, Zhao L, Villasmil R, Campos M, Amaral J, et al. Absence of TGFβ signaling in retinal microglia induces retinal degeneration and exacerbates choroidal neovascularization. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 1a	Gardner P, Liyanage S, Cristante E, Sampson R, Dick A, Ali R, et al. Hypoxia inducible factors are dispensable for myeloid cell migration into the inflamed mouse eye. Sci Rep. 2017;7:40830 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:250; fig s1	Szalay G, Martinecz B, Lénárt N, Kornyei Z, Orsolits B, Judák L, et al. Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke. Nat Commun. 2016;7:11499 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 3a	Tzekov R, Dawson C, Orlando M, Mouzon B, Reed J, Evans J, et al. Sub-Chronic Neuropathological and Biochemical Changes in Mouse Visual System after Repetitive Mild Traumatic Brain Injury. PLoS ONE. 2016;11:e0153608 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250	Crawford G, Boldison J, Copland D, Adamson P, Gale D, Brandt M, et al. The role of lipoprotein-associated phospholipase A2 in a murine model of experimental autoimmune uveoretinitis. PLoS ONE. 2015;10:e0122093 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:250	Lee S, Xu G, Jay T, Bhatta S, Kim K, Jung S, et al. Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway. J Neurosci. 2014;34:12538-46 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 2	Lambertsen K, Østergaard K, Clausen B, Hansen S, Stenvang J, Thorsen S, et al. No effect of ablation of surfactant protein-D on acute cerebral infarction in mice. J Neuroinflammation. 2014;11:123 pubmed publisher
immunohistochemistry - frozen section; mouse	Hultman K, Cortes Canteli M, Bounoutas A, Richards A, Strickland S, Norris E. Plasmin deficiency leads to fibrin accumulation and a compromised inflammatory response in the mouse brain. J Thromb Haemost. 2014;12:701-12 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:250	Bhaskar K, Maphis N, Xu G, Varvel N, Kokiko Cochran O, Weick J, et al. Microglial derived tumor necrosis factor-? drives Alzheimer's disease-related neuronal cell cycle events. Neurobiol Dis. 2014;62:273-85 pubmed publisher
	Markova L, Umek N, Horvat S, Hadzic A, Kuroda M, Pintaric T, et al. Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice. BMC Vet Res. 2020;16:247 pubmed publisher
	Boza Serrano A, Ruiz R, Sanchez Varo R, García Revilla J, Yang Y, Jimenez Ferrer I, et al. Galectin-3, a novel endogenous TREM2 ligand, detrimentally regulates inflammatory response in Alzheimer's disease. Acta Neuropathol. 2019;: pubmed publisher
	Sawicki C, Kim J, Weber M, Faw T, McKim D, Madalena K, et al. Microglia Promote Increased Pain Behavior through Enhanced Inflammation in the Spinal Cord during Repeated Social Defeat Stress. J Neurosci. 2019;39:1139-1149 pubmed publisher
	Bemiller S, Maphis N, Formica S, Wilson G, Miller C, Xu G, et al. Genetically enhancing the expression of chemokine domain of CX3CL1 fails to prevent tau pathology in mouse models of tauopathy. J Neuroinflammation. 2018;15:278 pubmed publisher
	Liao F, Li A, Xiong M, Bien Ly N, Jiang H, Zhang Y, et al. Targeting of nonlipidated, aggregated apoE with antibodies inhibits amyloid accumulation. J Clin Invest. 2018;128:2144-2155 pubmed publisher
	He P, Cheng X, Staufenbiel M, Li R, Shen Y. Long-term treatment of thalidomide ameliorates amyloid-like pathology through inhibition of ?-secretase in a mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e55091 pubmed publisher
	Kou J, Song M, Pattanayak A, Lim J, Yang J, Cao D, et al. Combined treatment of A? immunization with statin in a mouse model of Alzheimer's disease. J Neuroimmunol. 2012;244:70-83 pubmed publisher
	Gruenenfelder F, Thomson G, Penderis J, Edgar J. Axon-glial interaction in the CNS: what we have learned from mouse models of Pelizaeus-Merzbacher disease. J Anat. 2011;219:33-43 pubmed publisher
	Lodygensky G, West T, Moravec M, Back S, Dikranian K, Holtzman D, et al. Diffusion characteristics associated with neuronal injury and glial activation following hypoxia-ischemia in the immature brain. Magn Reson Med. 2011;66:839-45 pubmed publisher
	Jørgensen L, Blain A, Greally E, Laval S, Blamire A, Davison B, et al. Long-term blocking of calcium channels in mdx mice results in differential effects on heart and skeletal muscle. Am J Pathol. 2011;178:273-83 pubmed publisher
	Santos A, Martín Oliva D, Ferrer Martín R, Tassi M, Calvente R, Sierra A, et al. Microglial response to light-induced photoreceptor degeneration in the mouse retina. J Comp Neurol. 2010;518:477-92 pubmed publisher
	Clausen B, Lambertsen K, Babcock A, Holm T, Dagnaes Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008;5:46 pubmed publisher
	Li L, Liu J, Suo W. GRK5 deficiency exaggerates inflammatory changes in TgAPPsw mice. J Neuroinflammation. 2008;5:24 pubmed publisher
	Sato E, Shick H, Ransohoff R, Hirose K. Repopulation of cochlear macrophages in murine hematopoietic progenitor cell chimeras: the role of CX3CR1. J Comp Neurol. 2008;506:930-42 pubmed
	Santos A, Calvente R, Tassi M, Carrasco M, Martín Oliva D, Marín Teva J, et al. Embryonic and postnatal development of microglial cells in the mouse retina. J Comp Neurol. 2008;506:224-39 pubmed
	He P, Zhong Z, Lindholm K, Berning L, Lee W, Lemere C, et al. Deletion of tumor necrosis factor death receptor inhibits amyloid beta generation and prevents learning and memory deficits in Alzheimer's mice. J Cell Biol. 2007;178:829-41 pubmed
	Yang Y, Varvel N, Lamb B, Herrup K. Ectopic cell cycle events link human Alzheimer's disease and amyloid precursor protein transgenic mouse models. J Neurosci. 2006;26:775-84 pubmed

image

image 1 :

Published customer image: . Rat anti Mouse CD45 antibody, clone IBL-3/16 used for the detection of mouse leukocytes by flow cytometry. Image caption: . Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+ . (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 μm (A), 10 μm (B, C). 50 μm (I-P) *P J Neuroinflammation. 2008 Oct 23;5:46.

product information

Entity Type :

Monoclonal Antibody

Entity Category :

Antibodies

ProductCode :

MCA1388

Description :

Rat anti Mouse CD45

Specificity :

CD45

TargetSpecies :

Mouse

Host :

Rat

Format :

Purified

Isotypes :

IgG1

Applications :

WB,P,IP,F,C,IF,RE

ApplicationTypes :

Western Blotting,Immunohistology - Paraffin,Immunoprecipitation,Flow Cytometry,Immunohistology - Frozen,Immunofluorescence,Immunohistology - Resin

Cross Reactivities :

Rat,Human,Chicken

Clone :

IBL-3/16

Quantity :

0.25 mg

Quantity Value :

0.25

Quantity Type :

GO Accessions :

GO:0000187,GO:0001915,GO:0001916,GO:0001960,GO:0002925,GO:0051726,GO:0004725,GO:0005887,GO:0005925,GO:0006469,GO:0006470,GO:0007159,GO:0008201,GO:0009897,GO:0019901,GO:0030183,GO:0030890,GO:0031953,GO:0034113,GO:0042098,GO:0042100,GO:0043065,GO:0043395,GO:0043410,GO:0045059,GO:0045060,GO:0045121,GO:0045577,GO:0045588,GO:0046641,GO:0048304,GO:0050732,GO:0050852,GO:0050853,GO:0050855,GO:0050857,GO:0051209,GO:0051607

GOTerms :

activation of MAPK activity,negative regulation of T cell mediated cytotoxicity,positive regulation of T cell mediated cytotoxicity,negative regulation of cytokine-mediated signaling pathway,positive regulation of humoral immune response mediated by circulating immunoglobulin,regulation of cell cycle,protein tyrosine phosphatase activity,integral to plasma membrane,focal adhesion,negative regulation of protein kinase activity,protein dephosphorylation,leukocyte cell-cell adhesion,heparin binding,external side of plasma membrane,protein kinase binding,B cell differentiation,positive regulation of B cell proliferation,negative regulation of protein autophosphorylation,heterotypic cell-cell adhesion,T cell proliferation,B cell proliferation,positive regulation of apoptosis,heparan sulfate proteoglycan binding,positive regulation of MAPKKK cascade,positive thymic T cell selection,negative thymic T cell selection,membrane raft,regulation of B cell differentiation,positive regulation of gamma-delta T cell differentiation,positive regulation of alpha-beta T cell proliferation,positive regulation of isotype switching to IgG isotypes,negative regulation of peptidyl-tyrosine phosphorylation,T cell receptor signaling pathway,B cell receptor signaling pathway,regulation of B cell receptor signaling pathway,positive regulation of antigen receptor-mediated signaling pathway,release of sequestered calcium ion into cytosol,defense response to virus

Uniprot Accessions :

P06800

Gene :

Ptprc

GeneID URL :

http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=19264

Published Synonyms :

LCA

Entity Formats :

Purified

UNSPSC :

41116161

Concentration :

IgG concentration 0.5 mg/ml

Product Form :

Purified IgG - liquid

PriceGBP :

266

PriceEUR :

372

PriceUSD :

372 USD

PriceCHF :

405

PriceSEK :

4055

PriceNOK :

4075

PriceDKK :

2774

more info or order :

Bio-Rad product webpage