Published customer image: . Immunohistochemical staining of formalin fixed, paraffin embedded murine colonic tissue sections for zonulin-1 and occludin using Permanent aqueous mounting medium (BUF058) as a mountant. Image caption: . Bl 7952 induces upregulation of zonulin-1 and occludin in colon. Mice were treated with Bl 7952 (n=10), with Bl 372 (n=8) or with PBS (n=10) on ten consecutive days or were left untreated (Na n = 5). Colonic inflammation was induced by addition of 2.5% (w/v) DSS in the drinking water for 7 days. (A) Immunohistochemical detection of occludin and zonulin-1 proteins on representative paraffin-embedded sections of colon. (B) Representative western blotting of occludin and zonulin-1 proteins in the colonic mucosa. Expression of -actin was used as internal control. (C) Evaluation of intestinal permeability by FITC-dextran. Serum levels of 4.0-kDa FITC-dextran were measured in na ve controls (n=5), PBS/DSS-treated (n=5) and Bl 7952/DSS-treated mice (n=5) 5 hours after its intragastric administration. Data are express as mean SEM. Unpaired Student s t-test was used for comparison of Bl 7952/DSS group vs. control PBS/DSS group (**p 0.01). From: Srutkova D, Schwarzer M, Hudcovic T, Zakostelska Z, Drab V, Spanova A, et al . (2015) Bifidobacterium longum CCM 7952 Promotes Epithelial Barrier Function and Prevents Acute DSS-Induced Colitis in Strictly Strain-Specific Manner. PLoS ONE 10(7): e0134050 .