Published customer image: . Rabbit anti Chicken interferon-gamma antibody ( AHP945Z ) used for the evaluation of interferon- producing T-cells by flow cytometry. Image caption: . (A) The frequency of IFN- + CD4 + or IFN- + CD8 + T cells in lungs. Chickens in groups of five were vaccinated with mock vaccine or SD/01/10-ca on day 0 and challenged intranasally with 10 6 EID 50 of A/chicken/Hebei/YT/2010 on day 14 after vaccination. Lungs were harvested on day 7 after challenge. IFN- + CD4 + or IFN- + CD8 + T cells in lungs stimulated with SD/01/10-wt were measured. Percentages of IFN- + CD4 + or IFN- + CD8 + T cells within CD4 + or CD8 + T cells were analyzed. *P 0.05, by one-way analysis of variance of the difference between SD/01/10-ca and mock vaccine group on day 7 after challenge. (B) HI antibody responses to homologous and heterologous viruses after vaccination with SD/01/10-ca. Serum samples were collected on day 14 post immunization, and the reactivity of collected sera (n = 3) to SD/01/10-wt or five heterologous H9N2 influenza viruses was assessed by an HI assay. Abbreviations: BJ, Beijing; Ck, chicken; GD, Guangdong; HeB, Hebei; SD, Shandong. From: Wei Y, Qi L, Gao H, Sun H, Pu J, Sun Y, Liu J. Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine. Sci Rep. 2016 Jul 26;6:30382 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .