Published customer image: . Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated aortic cell preparations by immunofluorescence. Image caption: . Characterization of the isolated cells. (A) Representative phase contrast micrographs show the cell population collected at each of the four consecutive rounds of digestion (well 1 to 4) on the intimal (I) and adventitial (A) side of the aorta. In both cases, three main phenotypes were observed: small polygonal cells forming areas of cobblestone appearance, giant mono- or multinucleated cells and elongated cells. All three phenotypes were also detected at P1. Immunofluorescence analysis performed on each P0 population identified ECs by positive vWf staining (insert). The small polygonal mononucleated cells and the giant mono- or polynucleated morphologies, stained both for vWf. Rare elongated cells could also be vWf positive, their unusual shape likely being due to surrounding peer pressure. Magnification of the phase contrast pictures and immunofluorescence images is 10x and 63x, respectively. (B) Immunofluorescent micrographs showing the diversity of vWf presentation. vWf staining performed on adventitial aortic endothelial cells shows diverse and typical vWF staining patterns such as that of elongated WPBs throughout the cell body (d and e), residual vWf granules after degranulation of WPB or seen in cross section (a), reticular vWf around the nucleus corresponding to vWf re-synthesis after degranulation (b and c) or extracellular string of vWf (e). (C) Immunofluorescent micrographs of isolated cells double-stained for vWf together with either CD31 or VE-cadherin validates vWf staining. Small polygonal mono-nucleated cells and most of the giant mono- or polynucleated vWf positive cells also stained for CD31 or VE-cadherin at P0 and P2 and in IEC and AEC. From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al . (2015). A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta. PLoS ONE 10(11): e0143144 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .