Published customer image: . Rabbit anti Mouse MCP-1 antibody used for the identification of MCP-1 expressing cells in murine glioma by indirect immunofluorescence on formailin fixed vibratome sections. Image caption: . CCL2 + cells are GFAP + astrocytes in human glioma. (A) Confocal images show co-localization of CCL2-expressing cells (red) with GFAP (green) in glioma. Insert shows a detail of a single CCL2-expressing cell (red) co-localizing with GFAP (green) and counterstained with DAPI (blue). Scale bar: 30 um. (B) Areas of infiltration of CD3 + T-cells (green) coincide with the areas of CCL2-expressing cells (red) in glioma. Confocal images show a tumorigenic area, infiltrated with CD3 + T-cells (green) in an area with numerous CCL2-expressing cells (red). The immunostaining was combined with a counterstaining with DAPI to detect the nucleus (blue). Scale bar: 50 um. (C) Examples of characteristic CD3 infiltration in samples of glioma. CD3 + T-cells can be observed grouped in BVs (BV, limited by broken red line in 1 and 3) but also infiltrated in the parenchyma (2, 4, blue insert magnified in 5). (D) CCL2 expression correlates with the infiltration of T-cells in the tumor areas. The quantification in serial sections of the number of T-cells, either infiltrated or located in the BV lumen, revealed that the level of infiltration of CD3 + T-cells in the parenchyma (green) is positively correlated with the level of CCL2 expression (BV; BV lumen). From: Citation: Carrillo-de Sauvage MA, G mez A, Ros CM, Ros-Bernal F, Mart n ED, Perez-Vall s A, et al. (2012). CCL2-Expressing Astrocytes Mediate the Extravasation of T Lymphocytes in the Brain. Evidence from Patients with Glioma and Experimental Models In Vivo . PLoS ONE 7(2): e30762. doi:10.1371/journal.pone.0030762 .