Published customer image: . Mouse anti Yellow Fever virus antibody, clone 2D12 used for the detection of yellow fever envelope protein in infected dendritic cells by flow cytometry. Image caption: . Monocyte derived dendritic cells (MoDCs) infection with dengue virus 2 (DENV2) and yellow fever (YF) 17DD. MoDCs were infected with DENV2 or YF17DD at equivalent multiplicities of infections (MOIs) [2.5 x 10 5 50% tissue culture infectious dose (TCID)/ml] or YF17DD at higher MOI (1.25 x 10 6 TCID 50 /ml) or mock infected. Cells were labelled with anti DENV or anti YF antibodies using triplicates for each peripheral blood mononuclear cell (PBMC) donor. A: flow cytometry patterns for viral antigens on gated MoDCs 24 h after viral infection. Histograms demonstrate cell distribution by Alexa Fluor-488 fluorescence intensity (FL1-H: level of fluorescence). Cells were cultured with mock (dark grey) or DENV2 or YF17DD (light grey) at concentrated (conc) or equivalent (equiv) doses; B: viral antigen detection kinetics on infected MoDcs (data from four PBMC donors) [hpi: hours post-infection; X axis: time after infection (hours); Y axis: percentage of viral antigen positive cells (Ag + )]; C: intracellular viral antigen detection 24h after infection representing 11 cell donors (horizontal lines: distribution mean; X axis: viral inoculum; Y axis: percentage of viral Ag + cells. Asterisks mean: p 0.0001 in one way analysis of variance and Tukey s multiple comparison test). From: Gandini M, Reis SR, Torrentes-Carvalho A, Azeredo EL, Freire Mda S, Galler R, Kubelka CF. Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-α and IFN-α profiles. Mem Inst Oswaldo Cruz. 2011 Aug;106(5):594-605 .