Rat anti Human TNF Alpha
Bio-Rad
catalog: MCA1560
rat monoclonal (MP9-20A4)
reactivity: human
application: western blot, ELISA, flow cytometry, blocking or activating experiments
citations: 2

Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat IgG1 ( MCA6004PE ). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 0.5 mg
price: 462 USD
to the supplier
Mouse anti Human TNF Alpha
Bio-Rad
catalog: OBT1154
mouse monoclonal (195)
reactivity: human
application: western blot
citations: 1
quantity: 0.2 mg
price: 782 USD
to the supplier
Human anti Infliximab
Bio-Rad
catalog: HCA213G
human monoclonal (AbD17841_hIgG1)
reactivity: human
application: ELISA
citations: 1

HCA213 Specificity ELISA To confirm antibody specificity, a microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated HCA213 at 2μg/ml followed by QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 6200 USD
to the supplier
Mouse anti Human TNF Alpha
Bio-Rad
catalog: MCA747
mouse monoclonal (B-C7)
reactivity: human
application: ELISA
quantity: 0.5 mg
price: 998 USD
to the supplier
Goat anti Human TNF Alpha
Bio-Rad
catalog: AHP1212
domestic goat polyclonal
reactivity: human
application: western blot, ELISA
quantity: 0.1 mg
price: 526 USD
to the supplier
Goat anti Human TNF Alpha:Biotin
Bio-Rad
catalog: AHP1212B
domestic goat polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
quantity: 50 µg
price: 497 USD
to the supplier
Human anti Etanercept
Bio-Rad
catalog: HCA276
human monoclonal (AbD25939)
reactivity: human
application: ELISA

Human anti etanercept specificity ELISA A microtiter plate was coated overnight with various antigens at a concentration of 5 ug/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti etanercept (clone AbD25939) at a concentration of 2 ug/ml followed by a HRP conjugated anti-DYKDDDDK tag antibody in HISPEC diluent ( BUF049A ) and QuantaBlu fluorogenic peroxidase substrate.
quantity: 0.1 mg
price: 562 USD
to the supplier
Human anti Etanercept
Bio-Rad
catalog: HCA277
human monoclonal (AbD25940_hIgG4_Pro)
reactivity: human
application: ELISA

Human anti etanercept specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 ug/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD25940_hIgG4_Pro, HCA277P ) at a concentration of 2 ug/ml in HISPEC diluent ( BUF049A ) and QuantaBlu fluorogenic peroxidase substrate.
quantity: 0.1 mg
price: 652 USD
to the supplier
Human anti Etanercept:HRP
Bio-Rad
catalog: HCA277P
human monoclonal (AbD25940_hIgG4_Pro)
reactivity: human
conjugate: HRP
application: ELISA

Human anti etanercept specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 ug/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD25940_hIgG4_Pro, HCA277P ) at a concentration of 2 ug/ml in HISPEC diluent ( BUF049A ) and QuantaBlu fluorogenic peroxidase substrate.
quantity: 0.1 mg
price: 728 USD
to the supplier
Human anti Etanercept
Bio-Rad
catalog: HCA279
human monoclonal (AbD26183_hIgG1)
reactivity: human
application: ELISA

Human anti etanercept specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 ug/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD26183_hIgG1, HCA279P ) at a concentration of 2 ug/ml in HISPEC diluent ( BUF049A ) and QuantaBlu fluorogenic peroxidase substrate
quantity: 0.1 mg
price: 659 USD
to the supplier
Human anti Etanercept:HRP
Bio-Rad
catalog: HCA279P
human monoclonal (AbD26183_hIgG1)
reactivity: human
conjugate: HRP
application: ELISA

Human anti etanercept specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 ug/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti etanercept (clone AbD26183_hIgG1, HCA279P ) at a concentration of 2 ug/ml in HISPEC diluent ( BUF049A ) and QuantaBlu fluorogenic peroxidase substrate
quantity: 0.1 mg
price: 735 USD
to the supplier
Human anti Adalimumab
Bio-Rad
catalog: HCA202G
human monoclonal (AbD18654)
reactivity: human
application: ELISA

Human Anti-Adalimumab Antibody specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Adalimumab Antibody, clone AbD18654 (HCA202) was added at a concentration of 2 μg/ml. Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody ( MCA5995P ) in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 5279 USD
to the supplier
Human anti Adalimumab
Bio-Rad
catalog: HCA203G
human monoclonal (AbD18654_hIgG1)
reactivity: human
application: ELISA

Human Anti-Adalimumab Antibody specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, HRP conjugated Human Anti-Adalimumab Antibody, clone AbD18654_hIgG1 (HCA203) was added at a concentration of 2 μg/ml. Detection was performed using QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 6200 USD
to the supplier
Human anti Adalimumab
Bio-Rad
catalog: HCA204G
human monoclonal (AbD18655_hIgG1)
reactivity: human
application: ELISA

Human Anti-Adalimumab Antibody specificity ELISA . A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Adalimumab:HRP Antibody, clone AbD18655_hIgG1 ( HCA204P ) was added at a concentration of 2 μg/ml. Detection was performed using QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 6200 USD
to the supplier
Human anti Infliximab
Bio-Rad
catalog: HCA212G
human monoclonal (AbD17837)
reactivity: human
application: ELISA

HCA212 Specificity ELISA To confirm antibody specificity, a microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HCA212 at 2μg/ml, followed by Mouse anti Strep-tag classic:HRP ( MCA2489P ) in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 5279 USD
to the supplier
Human anti Infliximab
Bio-Rad
catalog: HCA214G
human monoclonal (AbD19376)
reactivity: human
application: ELISA

HCA214 Specificity ELISA To confirm antibody specificity, a microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HCA214 at 2μg/ml, followed by Mouse Anti-Histidine-tag:HRP Antibody in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate.
quantity: 1 mg
price: 5331 USD
to the supplier
Human anti Infliximab
Bio-Rad
catalog: HCA233G
human monoclonal (AbD20436_hIgG1)
reactivity: human
application: ELISA

Human Anti-Infliximab specificity ELISA A microtiter plate was coated over night with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human Anti-Infliximab Antibody (clone AbD20436_hIgG1) at a concentration of 2 μg/ml followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.
quantity: 1 mg
price: 6200 USD
to the supplier
Rat anti Human TNF Alpha:Alexa Fluor® 488
Bio-Rad
catalog: MCA1560A488
rat monoclonal (MP9-20A4)
reactivity: human
conjugate: AF488
application: flow cytometry

Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat IgG1 ( MCA6004PE ). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier
Rat anti Human TNF Alpha:Alexa Fluor® 647
Bio-Rad
catalog: MCA1560A647
rat monoclonal (MP9-20A4)
reactivity: human
conjugate: AF647
application: flow cytometry

Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat IgG1 ( MCA6004PE ). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier
Mouse anti Human TNF Alpha
Bio-Rad
catalog: MCA6435GA
mouse monoclonal (M22-1E1)
reactivity: human
application: ELISA, flow cytometry

Figure A. Unstimulated cells were stained with RPE conjugated Mouse anti Human CD3 ( MCA6146PE ) and Mouse anti Human TNF alpha ( MCA6435GA ) detected with Goat anti Mouse IgG1: FITC ( STAR132F ). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with RPE conjugated Mouse anti Human CD3 ( MCA6146PE ) and Mouse IgG1 isotype control ( MCA928 ) detected with Goat anti Mouse IgG1: FITC ( STAR132F ). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with RPE conjugated Mouse anti Human CD3 ( MCA6146PE ) and Mouse anti Human TNF alpha ( MCA6435GA ) detected with Goat anti Mouse IgG1: FITC ( STAR132F ). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
quantity: 0.1 mg
price: 249 USD
to the supplier