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Mouse anti Human Dectin-1
Bio-Rad
catalog: MCA4662
mouse monoclonal (BD6)
reactivity:
human
application:
immunocytochemistry
,
flow cytometry
citations: 1
Staining of human peripheral blood monocytes with Mouse anti Human Dectin-1:FITC (MCA4662F)
quantity: 0.2 mg
price: 372 USD
to the supplier
Mouse anti Human Dectin-1
Bio-Rad
catalog: MCA4662GA
mouse monoclonal (BD6)
reactivity:
human
application:
immunocytochemistry
,
flow cytometry
citations: 1
Staining of human peripheral blood monocytes with Mouse anti Human Dectin-1:FITC (MCA4662F)
quantity: 0.1 mg
price: 249 USD
to the supplier
Mouse anti Human Dectin-1:FITC
Bio-Rad
catalog: MCA4661F
mouse monoclonal (GE2)
reactivity:
human
conjugate: FITC
application:
flow cytometry
citations: 1
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 0.1 mg
price: 316 USD
to the supplier
Mouse anti Human Dectin-1:Low Endotoxin
Bio-Rad
catalog: MCA4661EL
mouse monoclonal (GE2)
reactivity:
human
application:
flow cytometry
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 0.5 mg
price: 622 USD
to the supplier
Mouse anti Human Dectin-1:FITC
Bio-Rad
catalog: MCA4661FT
mouse monoclonal (GE2)
reactivity:
human
conjugate: FITC
application:
flow cytometry
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 25 µg
price: 100 USD
to the supplier
Mouse anti Human Dectin-1
Bio-Rad
catalog: MCA4661GA
mouse monoclonal (GE2)
reactivity:
human
application:
flow cytometry
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 0.1 mg
price: 249 USD
to the supplier
Mouse anti Human Dectin-1:Alexa Fluor® 488
Bio-Rad
catalog: MCA4661A488
mouse monoclonal (GE2)
reactivity:
human
conjugate: AF488
application:
flow cytometry
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier
Mouse anti Human Dectin-1:Alexa Fluor® 647
Bio-Rad
catalog: MCA4662A647
mouse monoclonal (BD6)
reactivity:
human
conjugate: AF647
application:
flow cytometry
Staining of human peripheral blood monocytes with Mouse anti Human Dectin-1:FITC (MCA4662F)
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier
Mouse anti Human Dectin-1:RPE
Bio-Rad
catalog: MCA4661PE
mouse monoclonal (GE2)
reactivity:
human
conjugate: RPE
application:
flow cytometry
Published customer image: . Mouse anti Human Dectin-1 antibody, clone GE2 used in functional assays to assess bllocking activity for binding of A. fumigatus hyphae to human plasmacytoid dendritic cells. Image caption: . A. fumigatus hyphae are recognized by the mannose receptor, Dectin-2, on pDCs. Human pDCs were purified from PBMCs fractions using CD304-coated magnetic beads. (A) pDCs were treated with no inhibitors, dextran (1 mg/mL), mannan (1 mg/mL), laminarin (0.5 mg/mL) or mannan and laminarin for 30 minutes. The pDCs (5×104) were then incubated for 2 hr with A. fumigatus hyphae (5×104). The cell association was quantified by counting number of pDCs associated (touching or spreading over) with hyphae in 10 different fields. The percent cell association was calculated by dividing the number of pDC associated with hyphae by the total number of pDC counted and then multiplying by 100. (B) Same as in A except pDCs were treated with anti-Dectin-1 (100 μg/mL) and anti-Dectin-2 (100 μg/mL) antibodies for 30 minutes prior to incubation with hyphae. Data represent means ± SE of % cell association from three donors. *P PLoS Pathog 11(2): e1004643 .
quantity: 100 Tests
price: 392 USD
to the supplier