Mouse anti Human CD62E:FITC
Bio-Rad
catalog: MCA1969F

mouse monoclonal (CL2/6)
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 2

Published customer image: . Mouse anti Human CD62e antibody, clone CL2/6 ( MCA1969 ) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions. Image caption: . MSC-PPG-19Fc[FUT7 + ] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×10 6 MSCs/mL immobilized with either 100 ug/mL 19Fc[FUT7 + ], 100-200 ug/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm 2 (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm 2 (panel B). Only the MSC-PPG-19Fc[FUT7 + ] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean SD for 3-4 independent experiments. * indicates P 0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 um/s when MSC-PPG-19Fc[FUT7 + ] roll on P-selectin/CHO-P cells at 1 dyn/cm 2 and 11.5 um/s for rolling on E-selectin/ HUVECs at 2 dyn/cm 2 . The rolling characteristics of 190 cells were analyzed and binned from three independent runs. From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S. The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling. Biomaterials. 2013 Nov;34(33):8213-22 .
quantity: 100 Tests
price: 513 USD
to the supplier

Mouse anti Human CD62E/CD62P:FITC
Bio-Rad
catalog: MCA883F

mouse monoclonal (1.2B6)
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 1

Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P ( MCA883 )
quantity: 0.1 mg
price: 316 USD
to the supplier

Mouse anti Human CD62E/CD62P
Bio-Rad
catalog: MCA883

mouse monoclonal (1.2B6)
reactivity: human
application: western blot, ELISA, immunoprecipitation, flow cytometry
citations: 1

Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P ( MCA883 )
quantity: 0.2 mg
price: 372 USD
to the supplier

Mouse anti Human CD62E
Bio-Rad
catalog: MCA1969

mouse monoclonal (CL2/6)
reactivity: human
application: immunocytochemistry, flow cytometry

Published customer image: . Mouse anti Human CD62e antibody, clone CL2/6 ( MCA1969 ) used to affect CHO-P cell adhesion to selectin bearing substrate under shear stress conditions. Image caption: . MSC-PPG-19Fc[FUT7 + ] binding to P- and E-selectin in microfluidic flow chamber A-B. 2-5×10 6 MSCs/mL immobilized with either 100 ug/mL 19Fc[FUT7 + ], 100-200 ug/mL anti-P-selectin mAb G1 or 100-200 μg/mL anti-E-selectin mAb CL2/6 were perfused over either P-selectin bearing CHO-P cells at a wall shear stress of 1 dyn/cm 2 (panel A) or E-selectin bearing stimulated HUVECs at 2 dyn/cm 2 (panel B). Only the MSC-PPG-19Fc[FUT7 + ] cells bound the selectin bearing substrate. Cell interaction was specifically blocked using anti-PSGL-1 mAb KPL1, anti-P-selectin mAb G1 or anti-E-selectin mAb CL2/6 as indicated. Data are mean SD for 3-4 independent experiments. * indicates P 0.001 with respect to all other conditions. C. Cumulative rolling velocity plot showing median rolling velocity of 3 um/s when MSC-PPG-19Fc[FUT7 + ] roll on P-selectin/CHO-P cells at 1 dyn/cm 2 and 11.5 um/s for rolling on E-selectin/ HUVECs at 2 dyn/cm 2 . The rolling characteristics of 190 cells were analyzed and binned from three independent runs. From: Lo CY, Antonopoulos A, Dell A, Haslam SM, Lee T, Neelamegham S. The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling. Biomaterials. 2013 Nov;34(33):8213-22 .
quantity: 0.1 mg
price: 249 USD
to the supplier

Mouse anti Human CD62E/CD62P:FITC
Bio-Rad
catalog: MCA883FT

mouse monoclonal (1.2B6)
reactivity: human
conjugate: FITC
application: flow cytometry

Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P ( MCA883 )
quantity: 25 µg
price: 100 USD
to the supplier

Mouse anti Human CD62E/CD62P:RPE
Bio-Rad
catalog: MCA883PE

mouse monoclonal (1.2B6)
reactivity: human
conjugate: RPE
application: flow cytometry

Figure A. FITC conjugated Mouse anti Rat CD61 ( MCA2588F ) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ( MCA928A647 ). Figure B. FITC conjugated Mouse anti Rat CD61 ( MCA2588F ) and Alexa Fluor 647 conjugated Mouse anti Human CD62E/CD62P ( MCA883A647 ). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 100 Tests
price: 392 USD
to the supplier