mouse monoclonal (CC303)
reactivity: dog, cow
conjugate: RPE
application: flow cytometry
citations: 6
reactivity: dog, cow
conjugate: RPE
application: flow cytometry
citations: 6
Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 100 TESTS
price: 449 USD
to the supplier
mouse monoclonal (CC303)
reactivity: dog, cow
application: ELISA, flow cytometry
citations: 6
reactivity: dog, cow
application: ELISA, flow cytometry
citations: 6
Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 0.5 mg
price: 428 USD
to the supplier
goat polyclonal
reactivity: dog
application: western blot, ELISA
reactivity: dog
application: western blot, ELISA
Published customer image: . Horseradish peroxidase conjugated Goat anti Canine IgG1 ( AHP947P ) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting. Image caption: . IgG reaction pattern associated with CVL status. Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side. From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016). Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis. PLoS ONE 11(2): e0149894 .
quantity: 1 mg
price: 144 USD
to the supplier
goat polyclonal
reactivity: dog
conjugate: FITC
application: flow cytometry
reactivity: dog
conjugate: FITC
application: flow cytometry
Published customer image: . Horseradish peroxidase conjugated Goat anti Canine IgG1 ( AHP947P ) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting. Image caption: . IgG reaction pattern associated with CVL status. Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side. From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016). Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis. PLoS ONE 11(2): e0149894 .
quantity: 1 mg
price: 168 USD
to the supplier
mouse monoclonal (CC303)
reactivity: dog, cow
conjugate: FITC
application: flow cytometry
reactivity: dog, cow
conjugate: FITC
application: flow cytometry
Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 0.1 mg
price: 392 USD
to the supplier