Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and then stained with TMRM dye. Cells were analyzed using a Nikon Eclipse E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence
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Jurkat T cells were treated with DMSO or 50 uM CCCP for 1 hour followed by staining with Mitochondrial Permeability Transition: MitoPT JC-1 Kit ( ICT943 ) for 15-30 minutes. Loss of mitochondrial potential results in a reduction in red fluorescence and an increase in green fluorescence. Data acquired on the ZE5 Cell Analyzer.
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Jurkat cells were stained with MitoPT TM JC-1 and viewed through a fluorescence microscope using a broad band path filter. Non-apoptotic cells exhibit red stained mitochondria (2 cells at the top). Apoptotic cells at varying stages of mitochondrial membrane potential appear green (3 cells at the bottom)
quantity: 400 TESTS
price:
to the supplier