Mouse anti Human CD54
Bio-Rad
catalog: MCA1615

mouse monoclonal (15.2)
reactivity: human, rat
application: immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 7

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 0.2 mg
price: 372 USD
to the supplier

Mouse anti Human CD54:Low Endotoxin
Bio-Rad
catalog: MCA1615EL

mouse monoclonal (15.2)
reactivity: human
application: ELISA, immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 4

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 0.5 mg
price: 622 USD
to the supplier

Mouse anti Human CD54:FITC
Bio-Rad
catalog: MCA1615F

mouse monoclonal (15.2)
reactivity: human
conjugate: FITC
application: flow cytometry
citations: 2

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 0.1 mg
price: 316 USD
to the supplier

Mouse anti Human CD54
Bio-Rad
catalog: MCA532

mouse monoclonal (84H10)
reactivity: human
application: ELISA, immunoprecipitation, flow cytometry
citations: 3

Published customer image: . ICAM-1 activates PKC but not PKC II in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNF to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNF as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKC II and PKC was examined by western blot with B) anti-phospho-PKC IIThr641 or anti-PKC II or C–D) anti-phospho- PKC Thr638 or anti-PKC . Shown are representative blots. Shown are the mean SEM of 3 experiments. NT, nontreated. *, p 0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012). Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKC in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054 .
quantity: 0.2 mg
price: 462 USD
to the supplier

Mouse anti Human CD54
Bio-Rad
catalog: MCA1615GA

mouse monoclonal (15.2)
reactivity: human
application: immunocytochemistry, immunoprecipitation, flow cytometry
citations: 2

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 0.1 mg
price: 249 USD
to the supplier

Mouse anti Human CD54:RPE
Bio-Rad
catalog: MCA1615PE

mouse monoclonal (15.2)
reactivity: human
conjugate: RPE
application: flow cytometry
citations: 1

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 100 Tests
price: 392 USD
to the supplier

Mouse anti Human CD54
Bio-Rad
catalog: MCA1615T

mouse monoclonal (15.2)
reactivity: human
application: flow cytometry
citations: 1

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 20 µg
price: 100 USD
to the supplier

Mouse anti Human CD54
Bio-Rad
catalog: MCA532T

mouse monoclonal (84H10)
reactivity: human
application: ELISA, immunoprecipitation, flow cytometry
citations: 1

Published customer image: . ICAM-1 activates PKC but not PKC II in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNF to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNF as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKC II and PKC was examined by western blot with B) anti-phospho-PKC IIThr641 or anti-PKC II or C–D) anti-phospho- PKC Thr638 or anti-PKC . Shown are representative blots. Shown are the mean SEM of 3 experiments. NT, nontreated. *, p 0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012). Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKC in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054 .
quantity: 25 µg
price: 249 USD
to the supplier

Mouse anti Human CD54:Alexa Fluor® 647
Bio-Rad
catalog: MCA1615A647

mouse monoclonal (15.2)
reactivity: human
conjugate: AF647
application: flow cytometry

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier

Mouse anti Human CD54:Alexa Fluor® 647
Bio-Rad
catalog: MCA1615A647T

mouse monoclonal (15.2)
reactivity: human
conjugate: AF647
application: flow cytometry

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 25 Tests/0.25 ml
price: 143 USD
to the supplier

Mouse anti Human CD54:Pacific Blue®
Bio-Rad
catalog: MCA1615PB

mouse monoclonal (15.2)
reactivity: human
conjugate: Pacific Blue
application: flow cytometry

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier

Mouse anti Human CD54:Alexa Fluor® 488
Bio-Rad
catalog: MCA1615A488

mouse monoclonal (15.2)
reactivity: human
conjugate: AF488
application: flow cytometry

Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNF for 19 h then stained with RPE conjugated Mouse anti Human CD31 ( MCA1738PE ) and Alexa Fluor A700 conjugated Mouse anti Human CD54 ( MCA1615A700 ). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier