mouse monoclonal (CC149)
reactivity: bovine
conjugate: RPE-Cy5
application: flow cytometry
citations: 4
reactivity: bovine
conjugate: RPE-Cy5
application: flow cytometry
citations: 4
Published customer image: . Mouse anti Bovine CD172a antibody, clone CC149 ( MCA2041GA ) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry. Image caption: . BM of Csf1r-EGFP sheep contains a heterogeneous EGFP + population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFP lo and EGFP hi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFP lo and GFP hi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP + cells (combined EGFP lo and EGFP hi ). Scale bar, 10 um. Dot plots and Leishman staining of EGFP + cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal. From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep. J Immunol. 2016 Sep 15;197(6):2297-305 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 100 Tests/1 ml
price: 557 USD
to the supplier
mouse monoclonal (DH59B)
reactivity: bovine
application: flow cytometry
citations: 1
reactivity: bovine
application: flow cytometry
citations: 1
Flow cytometric staining of bovine peripheral blood monocytes. . A. Mouse IgG1 negative control ( MCA928 ) followed by Goat anti Mouse IgG1:PE ( STAR132PE ). B. Mouse anti Bovine CD172a antibody, clone DH59B (MCA6079) followed by Goat anti Mouse IgG1:PE secondary antibody ( STAR132PE ). Both the Mouse IgG1 negative control and Mouse anti Bovine CD172a are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 ( MCA1425F ).
quantity: 0.1 mg
price: 279 USD
to the supplier
mouse monoclonal (CC149)
reactivity: bovine
application: flow cytometry
reactivity: bovine
application: flow cytometry
Published customer image: . Mouse anti Bovine CD172a antibody, clone CC149 ( MCA2041GA ) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry. Image caption: . BM of Csf1r-EGFP sheep contains a heterogeneous EGFP + population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFP lo and EGFP hi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFP lo and GFP hi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP + cells (combined EGFP lo and EGFP hi ). Scale bar, 10 um. Dot plots and Leishman staining of EGFP + cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal. From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep. J Immunol. 2016 Sep 15;197(6):2297-305 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 0.1 mg
price: 288 USD
to the supplier
mouse monoclonal (CC149)
reactivity: bovine
conjugate: AF488
application: flow cytometry
reactivity: bovine
conjugate: AF488
application: flow cytometry
Published customer image: . Mouse anti Bovine CD172a antibody, clone CC149 ( MCA2041GA ) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry. Image caption: . BM of Csf1r-EGFP sheep contains a heterogeneous EGFP + population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFP lo and EGFP hi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFP lo and GFP hi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP + cells (combined EGFP lo and EGFP hi ). Scale bar, 10 um. Dot plots and Leishman staining of EGFP + cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal. From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep. J Immunol. 2016 Sep 15;197(6):2297-305 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 100 Tests/1 ml
price: 557 USD
to the supplier
mouse monoclonal (CC149)
reactivity: bovine
conjugate: AF647
application: flow cytometry
reactivity: bovine
conjugate: AF647
application: flow cytometry
Published customer image: . Mouse anti Bovine CD172a antibody, clone CC149 ( MCA2041GA ) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry. Image caption: . BM of Csf1r-EGFP sheep contains a heterogeneous EGFP + population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFP lo and EGFP hi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFP lo and GFP hi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP + cells (combined EGFP lo and EGFP hi ). Scale bar, 10 um. Dot plots and Leishman staining of EGFP + cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal. From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep. J Immunol. 2016 Sep 15;197(6):2297-305 . This is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 100 Tests/1 ml
price: 557 USD
to the supplier