rat monoclonal (YE2/36-HLK)
reactivity: human
application: flow cytometry
citations: 2
reactivity: human
application: flow cytometry
citations: 2
Published customer image: . Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry. Image caption: . BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP− population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody. From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009). The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255 .
quantity: 0.25 mg
price: 408 USD
to the supplier
mouse monoclonal (HL-39)
reactivity: human
application: flow cytometry
citations: 1
reactivity: human
application: flow cytometry
citations: 1
rat monoclonal (YE2/36-HLK)
reactivity: human
conjugate: RPE
application: flow cytometry
reactivity: human
conjugate: RPE
application: flow cytometry
Published customer image: . Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometry. Image caption: . BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP− population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody. From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. (2009). The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255 .
quantity: 100 Tests
price: 428 USD
to the supplier
rat monoclonal (YD1/63.4.10)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
Published customer image: . Rat anti Human HLA-DR antibody, clone YD1/63.4.10 used for the evaluation of MHC class II expression on human PBMC by flow cytometry. Image caption: . Plasmacytoid dendritic cells show weak T cell stimulatory capacity. A: Flow cytometric analysis of human PBMC and DC. pDC are characterized as lineage − CD11c − CD303 + cells (middle panel). Dot plots (right side) exemplarily depicts isolated pDC, percentage indicates purity. Histograms show surface expression of CD40, CD80, CD86, CD58 and HLA-DR on resting and CpG/IL-3-activated pDC and cytokine cocktail activated cDC. Isotype-matched control mAb staining is shown in grey. One representative result of three independent experiments is shown. From: Hubo M, Jonuleit H (2012) Plasmacytoid Dendritic Cells Are Inefficient in Activation of Human Regulatory T Cells. PLoS ONE 7(8): e44056 .
quantity: 0.2 mg
price: 408 USD
to the supplier