Mouse anti Bovine Interleukin-4:RPE
Bio-Rad
catalog: MCA1820PE

mouse monoclonal (CC303)
reactivity: dogs, bovine
conjugate: RPE
application: flow cytometry
citations: 6

Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 100 Tests
price: 557 USD
to the supplier

Mouse anti Bovine Interleukin-4
Bio-Rad
catalog: MCA1820

mouse monoclonal (CC303)
reactivity: bovine
application: western blot, ELISA, flow cytometry
citations: 7

Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 0.5 mg
price: 531 USD
to the supplier

Mouse anti Bovine Interleukin-4
Bio-Rad
catalog: MCA2371

mouse monoclonal (CC313)
reactivity: bovine
application: ELISA
citations: 5

Published customer image: . Mouse anti Bovine Interleukin-4 antibody, clone CC313 ( MCA2371 ) used as a capture reagent for the detection of interleukin-4 in supernatants of cultured bovine cells using a sandwich ELISA together with biotinylated clone CC314 ( MCA2372B ) as a detection reagent. Image caption: . CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 10 5 allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [ 3 H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN- (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C). From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012) Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells. PLoS ONE 7(1): e30430 .
quantity: 0.5 mg
price: 515 USD
to the supplier

Mouse anti Bovine Interleukin-4:Biotin
Bio-Rad
catalog: MCA2372B

mouse monoclonal (CC314)
reactivity: bovine
conjugate: biotin
application: ELISA
citations: 1

Published customer image: . Biotinylated Mouse anti Bovine interleukin-4 antibody, clone CC314 ( MCA2372B ) used as a detection reagen for the evaluation of IL-4 secretion by Lymph dendritic cells in vitro by ELISA in combination with Mouse anti Bovine interleukin-4, clone CC313 ( MCA2371 ) as a capture reagent. Image caption: . CD1b + L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b + L-DCs were incubated at different ratios with 10 5 allogeneic CD4 + T cells (A) or CD62L + T cells (C), and tested for proliferation by [ 3 H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN- (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C). From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012). Capacities of Migrating CD1b + Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells. PLoS ONE 7(1): e30430 .
quantity: 0.25 mg
price: 471 USD
to the supplier

Mouse anti Bovine Interleukin-4:FITC
Bio-Rad
catalog: MCA1820F

mouse monoclonal (CC303)
reactivity: bovine
conjugate: FITC
application: flow cytometry

Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF-α, IFN-γ, TGF-β and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 .
quantity: 0.1 mg
price: 486 USD
to the supplier

Mouse anti Bovine Interleukin-4:Alexa Fluor® 647
Bio-Rad
catalog: MCA1820A647

mouse monoclonal (CC303)
reactivity: bovine
conjugate: AF647
application: flow cytometry

Published customer image: . Mouse anti Bovine interleukin-4 antibody, clone CC303 ( MCA1820 ) used for the identification of interferon expressing dog lymphocytes by flow cytometry. Image caption: . Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines + (IL-17, TNF- , IFN- , TGF- and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines + (IL-17, TNF- , IFN- , TGF- and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 100 Tests/1 ml
price: 557 USD
to the supplier