domestic sheep polyclonal
application: ELISA, immunocytochemistry
citations: 4
application: ELISA, immunocytochemistry
citations: 4
Published customer image: . Sheep antiGreen Fluorescent protein antibody ( 4745-1051 ) used to detect GFP tagged constructs (CG8784:p65) in Drosophila larvae by immunofluorescence. Image caption: . Hugin neurons target neurosecretory cells via peptide-receptor transmission in addition to synaptic connections. A, Promoter-based hugin receptor PK2-R1 driver line CG8784-Gal4::p65 was generated by replacing the first coding exon of the CG8784 loci with GAL4 in a BAC clone containing 80kb flanking genomic context and integrating the final BAC into attP site VK00033. B, CG8784-Gal4::p65 drives expression in cells of the pars intercerebralis (PI). C, Co-staining with Drosophila insulin-like peptide 2 (Dilp2), diuretic hormone 44 (DH44) and Dromyosuppressin (DMS). These peptides are produced by medial neurosecretory cells (mNSCs) in a non-overlapping manner. CG8784-Gal4::p65 drives expression in all mNSCs of the PI. Scale bars in A and B represent 10 &mum. From: Schlegel P, Texada MJ, Miroschnikow A, Schoofs A, H ckesfeld S, Peters M,Schneider-Mizell CM, Lacin H, Li F, Fetter RD, Truman JW, Cardona A, Pankratz MJ. Synaptic transmission parallels neuromodulation in a central food-intake circuit. Elife. 2016 Nov 15;5. pii: e16799 .
quantity: 1 ml
price: 326 USD
to the supplier
domestic rabbit polyclonal
application: western blot
citations: 2
application: western blot
citations: 2
domestic goat polyclonal
application: western blot, ELISA, immunocytochemistry
citations: 1
application: western blot, ELISA, immunocytochemistry
citations: 1
Published customer image: . Sheep anti Green Fluorescent Protein antibody used for the identification of GFP tagged VEGF-A expressing cells by immunofluorescence. Image caption: . HSV-1 infection drives transcriptional upregulation of VEGF-A. (A) Representative image of a cornea from pVEGFA-GFP reporter mice at day 3 PI with HSV-1 strain McKrae stained for HSV-1 antigen (red), GFP transcriptional reporter for VEGF-A (green) and DAPI (blue) (B) GFP transcriptional reporter expression (green) with HSV-1 antigen (red) at 12 hours PI with HSV-1 strain McKrae. (C) Real time PCR of THCE cells at 12 hours PI with 3 pfu per cell HSV-1 strain McKrae with VEGF-A fold induction determined via the geometric means of VEGF-A induction relative to the housekeeping genes -actin, TBP and PPIA (** p 0.01). (D) Real time PCR of VEGF-A transcription in 293 cells infected with 3 pfu per cell HSV-1 strain McKrae also determined via geometric mean relative to -actin, TBP and PPIA (** p 0.01) and (E) Levels of VEGF-A by cytokine bead array in cytoplasmic extracts of 293 cells at the indicated times PI with 3 pfu per cell HSV-1 strain McKrae, expressed as pg of VEGF-A per ug of cytoplasmic protein extract. (F) Transcriptional activity of the proximal human VEGF-A promoter was measured using a luciferase vector with luciferase driven by the VEGF-A promoter (spanning base pairs −2018 to +50 bp relative to the transcription start site). Transfected 293 cells were infected with 3 pfu per cell of either wild-type HSV-1 strains; McKrae, KOS, or SC16 or with 3 pfu per cell HSV-2 and assayed for luciferase activity at the indicated time PI. Luciferase activity was normalized to the activity of uninfected 293 cells transfected with a promoterless luciferase vector, pGL3 (** p 0.01). All figures are representative figures from individual experiments with an n = 3/group for A–D. Extracts from 3 culture wells per time point were pooled for E. Bars denote mean VEGF-A pg per ug of cytoplasmic protein SEM. From: Wuest T, Zheng M, Efstathiou S, Halford WP, Carr DJJ (2011). The Herpes Simplex Virus-1 Transactivator Infected Cell Protein-4 Drives VEGF-A Dependent Neovascularization. PLoS Pathog 7(10): e1002278 .
quantity: 0.1 mg
price: 195 USD
to the supplier
mouse monoclonal (N/K)
application: western blot, ELISA
application: western blot, ELISA