domestic sheep polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Published customer image: . Sheep anti Human alkaline phosphatase antibody used for the detection of alkaline phosphatase in Caco-2/TC7 cells by western blotting. Image caption: . PPM supply induces movement of SR-BI/CLA-1 towards raft microdomains. (A) Caco-2/TC7 cells were harvested in the presence of Triton X-100 and the lysate fractionated on a 5–40% sucrose gradient. Eleven fractions were collected for immunoblots of SR-BI/CLA-1, EEA1 (early endosome antigen 1) and flottilin-1 (raft marker). (B) Caco-2/TC7 cells were cultured in the absence (control) or presence of PPM or IPM for 10 min and then harvested in the presence of Triton X-100. Cell lysates were applied to a 5–40% sucrose gradient and eleven fractions collected. Fractions 3 to 8 were analyzed by immunoblotting with antibodies against SR-BI/CLA-1 (left panel) and flottilin-1 (right panel). (C) Immunolocalization of SR-BI/CLA-1 and alkaline phosphatase (PLAP, used as raft marker) in the brush border of Caco-2/TC7 cells supplied with PPM. SR-BI/CLA-1 is labelled with anti-rabbit immunoglobulin-gold complexes (18-nm particles) and PLAP with anti-sheep immunoglobulin-gold complexes (12-nm particles). MV, microvilli; bar, 100 nm. From: B aslas O, Cueille C, Delers F, Chateau D, Chambaz J, Rousset M, et al. (2009). Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1. PLoS ONE 4(1): e4278 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 1 ml
price: 327 USD
to the supplier
mouse monoclonal (BGN/03/662)
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
mouse monoclonal (H17E2)
reactivity: human
application: ELISA, flow cytometry
reactivity: human
application: ELISA, flow cytometry
Published customer image: . Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 used for the detection of PLAP reporter expression on peripheral blood granulocytes by flow cytometrry. Image caption: . Figure 3. Activation of the Cre-reporter gene is less efficient in Z/AP and Z/EG mice than in R26R-EYFP mice. All reporter strains were crossed to the general deleter strain, pCX-NLS-Cre, and activation of each reporter gene was assessed by flow cytometry of peripheral blood leukocytes at 12 weeks of age (A). All reporter strains were also crossed to the myeloid-specific LysM-Cre strain, and activation of each reporter gene was assessed by flow cytometry of peripheral blood granulocytes at 12 weeks of age (B). Representative histograms demonstrating reporter expression in peripheral blood granulocytes are shown in (C–E) for LysM-Cre/R26R-EYFP, LysM-Cre/ZAP and LysM-Cre/Z/EG mice respectively (solid lines). Reporter expression in BL/6 mice is shown in dotted lines. From: Long MA, Rossi FMV (2009) Silencing Inhibits Cre-Mediated Recombination of the Z/AP and Z/EG Reporters in Adult Cells. PLoS ONE 4(5): e5435 .
quantity: 0.2 mg
price: 394 USD
to the supplier
mouse monoclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of whole cell lysates probed with placental alkaline phosphatase antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to placental alkaline phosphatase (molecular weight 70 kDa)
quantity: 100 µl
price: 346 USD
to the supplier