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Rat anti Mouse CD169
Bio-Rad
catalog: MCA884
rat monoclonal (3D6.112)
reactivity:
mouse
application:
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - frozen section
citations: 17
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 0.2 mg
price: 372 USD
to the supplier
Rat anti Mouse CD169
Bio-Rad
catalog: MCA947G
rat monoclonal (MOMA-1)
reactivity:
mouse
application:
immunocytochemistry
,
immunohistochemistry - frozen section
citations: 23
Published customer image: . AlexaFluor 647 conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used to demonstrate marginal zone metallophils by immunofluorescence. Image caption: . DCIR2 + cDCs migrate from the marginal zone bridging channel to the T cell zone after immunization. Spleens from BALB/c mice (n = 2 per time point) immunized with 250 ug IgE anti-OVA pre-mixed with 100 ug OVA or with 100 ug OVA alone were harvested after 0.5, 4, 8, 24, or 48 h. One unimmunized mouse (Nil) was used as control. (a–k) Half of each spleen was snap-frozen and non-consecutive spleen sections were stained and analyzed by confocal microscopy. Localization of DCIR2 + cDCs in spleens harvested at indicated time points after immunization was followed. B220 + B cells, blue; CD169 + metallophilic macrophages, grey; DCIR2 + cDCs, red. Marginal zone bridging channels are indicated with arrows in (a,g). Images show representative areas (640 um × 640 um) of 3–4 T cell zones from 2 non-consecutive sections of each sample in every group. Scale bar represents 100 um. Data represent one experiment where mice were immunized with IgE-OVA or OVA alone and one where they were immunized with IgE-OVA. (l) The other halves of the spleens from mice immunized with IgE-OVA complexes in (a–e) were prepared into single cell suspensions and 6 × 10 5 cells were used as APCs in co-cultures with 10 5 CFSE-labeled CD4 + T cells isolated from DO11.10 splenocytes. Percentages of divided cells among OVA-specific CD4+ T cells after incubation for 3 days with APCs taken from an unimmunized mouse (Nil) or from mice immunized with IgE-OVA complexes are quantified by flow cytometry as shown in Fig. 3. CD4 + T cells cultured alone were used as negative control. Each circle represents one mouse and the lines represent the mean values. From: Ding Z, Dahlin JS, Xu H, Heyman B. IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8 (-) conventional dendritic cells. Sci Rep. 2016 Jun 16;6:28290 .
quantity: 0.25 mg
price: 372 USD
to the supplier
Rat anti Mouse CD169
Bio-Rad
catalog: MCA947GA
rat monoclonal (MOMA-1)
reactivity:
mouse
application:
immunocytochemistry
,
immunohistochemistry - frozen section
citations: 21
Published customer image: . AlexaFluor 647 conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used to demonstrate marginal zone metallophils by immunofluorescence. Image caption: . DCIR2 + cDCs migrate from the marginal zone bridging channel to the T cell zone after immunization. Spleens from BALB/c mice (n = 2 per time point) immunized with 250 ug IgE anti-OVA pre-mixed with 100 ug OVA or with 100 ug OVA alone were harvested after 0.5, 4, 8, 24, or 48 h. One unimmunized mouse (Nil) was used as control. (a–k) Half of each spleen was snap-frozen and non-consecutive spleen sections were stained and analyzed by confocal microscopy. Localization of DCIR2 + cDCs in spleens harvested at indicated time points after immunization was followed. B220 + B cells, blue; CD169 + metallophilic macrophages, grey; DCIR2 + cDCs, red. Marginal zone bridging channels are indicated with arrows in (a,g). Images show representative areas (640 um × 640 um) of 3–4 T cell zones from 2 non-consecutive sections of each sample in every group. Scale bar represents 100 um. Data represent one experiment where mice were immunized with IgE-OVA or OVA alone and one where they were immunized with IgE-OVA. (l) The other halves of the spleens from mice immunized with IgE-OVA complexes in (a–e) were prepared into single cell suspensions and 6 × 10 5 cells were used as APCs in co-cultures with 10 5 CFSE-labeled CD4 + T cells isolated from DO11.10 splenocytes. Percentages of divided cells among OVA-specific CD4+ T cells after incubation for 3 days with APCs taken from an unimmunized mouse (Nil) or from mice immunized with IgE-OVA complexes are quantified by flow cytometry as shown in Fig. 3. CD4 + T cells cultured alone were used as negative control. Each circle represents one mouse and the lines represent the mean values. From: Ding Z, Dahlin JS, Xu H, Heyman B. IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8 (-) conventional dendritic cells. Sci Rep. 2016 Jun 16;6:28290 .
quantity: 0.1 mg
price: 249 USD
to the supplier
Rat anti Mouse CD169
Bio-Rad
catalog: MCA884GA
rat monoclonal (3D6.112)
reactivity:
mouse
application:
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - frozen section
citations: 13
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 0.1 mg
price: 249 USD
to the supplier
Rat anti Mouse CD169:Low Endotoxin
Bio-Rad
catalog: MCA884EL
rat monoclonal (3D6.112)
reactivity:
mouse
application:
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - frozen section
citations: 11
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 0.5 mg
price: 622 USD
to the supplier
Rat anti Mouse CD169:FITC
Bio-Rad
catalog: MCA947F
rat monoclonal (MOMA-1)
reactivity:
mouse
conjugate: FITC
application:
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - frozen section
citations: 6
Published customer image: . AlexaFluor 647 conjugated Rat anti Mouse CD169 antibody, clone MOMA-1 used to demonstrate marginal zone metallophils by immunofluorescence. Image caption: . DCIR2 + cDCs migrate from the marginal zone bridging channel to the T cell zone after immunization. Spleens from BALB/c mice (n = 2 per time point) immunized with 250 ug IgE anti-OVA pre-mixed with 100 ug OVA or with 100 ug OVA alone were harvested after 0.5, 4, 8, 24, or 48 h. One unimmunized mouse (Nil) was used as control. (a–k) Half of each spleen was snap-frozen and non-consecutive spleen sections were stained and analyzed by confocal microscopy. Localization of DCIR2 + cDCs in spleens harvested at indicated time points after immunization was followed. B220 + B cells, blue; CD169 + metallophilic macrophages, grey; DCIR2 + cDCs, red. Marginal zone bridging channels are indicated with arrows in (a,g). Images show representative areas (640 um × 640 um) of 3–4 T cell zones from 2 non-consecutive sections of each sample in every group. Scale bar represents 100 um. Data represent one experiment where mice were immunized with IgE-OVA or OVA alone and one where they were immunized with IgE-OVA. (l) The other halves of the spleens from mice immunized with IgE-OVA complexes in (a–e) were prepared into single cell suspensions and 6 × 10 5 cells were used as APCs in co-cultures with 10 5 CFSE-labeled CD4 + T cells isolated from DO11.10 splenocytes. Percentages of divided cells among OVA-specific CD4+ T cells after incubation for 3 days with APCs taken from an unimmunized mouse (Nil) or from mice immunized with IgE-OVA complexes are quantified by flow cytometry as shown in Fig. 3. CD4 + T cells cultured alone were used as negative control. Each circle represents one mouse and the lines represent the mean values. From: Ding Z, Dahlin JS, Xu H, Heyman B. IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8 (-) conventional dendritic cells. Sci Rep. 2016 Jun 16;6:28290 .
quantity: 0.1 mg
price: 316 USD
to the supplier
Rat anti Mouse CD169:FITC
Bio-Rad
catalog: MCA884F
rat monoclonal (3D6.112)
reactivity:
mouse
conjugate: FITC
application:
flow cytometry
,
immunohistochemistry - frozen section
citations: 3
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 0.1 mg
price: 316 USD
to the supplier
Rat anti Mouse CD169:FITC
Bio-Rad
catalog: MCA884FT
rat monoclonal (3D6.112)
reactivity:
mouse
conjugate: FITC
application:
flow cytometry
,
immunohistochemistry - frozen section
citations: 1
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 25 µg
price: 100 USD
to the supplier
Rat anti Mouse CD169:Alexa Fluor® 647
Bio-Rad
catalog: MCA884A647
rat monoclonal (3D6.112)
reactivity:
mouse
conjugate: AF647
application:
flow cytometry
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 100 Tests/1 ml
price: 392 USD
to the supplier
Rat anti Mouse CD169:RPE
Bio-Rad
catalog: MCA884PE
rat monoclonal (3D6.112)
reactivity:
mouse
conjugate: RPE
application:
flow cytometry
Figure A. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat IgG2a isotype control ( MCA1212F ). Figure B. RPE conjugated Rat anti Mouse CD45R ( MCA1258PE ) and FITC conjugated Rat anti Mouse CD169 ( MCA884F ). All experiments performed on murine bone marrow in the presence of murine SeroBlock ( BUF041A ).
quantity: 100 Tests
price: 392 USD
to the supplier