Published customer image: . Goat anti Rat calcitonin gene related peptide antibody used for the detection of CRGP expressing axons in rat spinal cord by immunofluorescence. Image caption: . Axon fiber composition in paravertebral ganglia and related labeling in spinal cord. A. Lower power image of two caudal sympathetic ganglia and connecting nerve bridge showing nerve contains a mixture of at least 3 neurochemically distinct fiber populations: HB9-GFP+ sympathetic preganglionic neurons (SPNs), CGRP+ visceral afferents, and TH+ sympathetic postganglionics. Boxed regions are enlarged in B and C. B. Enlargement showing SPNs (GFP+), CGRP and TH immunolabeled axons in axon bundle between two ganglia. Image represents composite of 73 consecutive confocal images taken at 0.38 µm optical section thickness (27.74 µm total thickness). C. Enlargement of SPNs (GFP+), CGRP and TH immunolabeling in the left sympathetic ganglion. Section in central region of ganglia shows that, while SPNs appear to form basket-like synapses around postganglionics, CGRP+ afferents do not project through this region. Image is a collapsed stack of 11 consecutive confocal images (4.18 µm total). D. CGRP (red) and HB9-GFP labeling (green) in a transverse section of thoracic spinal cord. Note predominant CGRP labeling in the dorsal horn. HB9-GFP labeling of SPNs in the intermediolateral nucleus and motoneurons (MNs) in the ventral horn are identified on the right side. Middle panel shows CGRP labeling extending ventrally including into the intermediolateral nucleus (at arrow) while right panel provides a higher magnification image showing CGRP labeling intermingled with SPNs in the intermediolateral nucleus as well as along its medially oriented SPN projections. Scale bar is 100 µm in A, 20 µm in B and C, and 50 µm in D. From: Zimmerman AL, Sawchuk M, Hochman S (2012) Monoaminergic Modulation of Spinal Viscero-Sympathetic Function in the Neonatal Mouse Thoracic Spinal Cord. PLoS ONE 7(11): e47213 .