Bio-Rad CD8 antibody

Rat anti Mouse CD8 Alpha
Bio-Rad
catalog: MCA609G

rat monoclonal (KT15)
reactivity: human, mouse
application: immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 7

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 0.25 mg
price: 408 USD
to the supplier

Rat anti Mouse CD8
Bio-Rad
catalog: MCA1108G

rat monoclonal (YTS105.18)
reactivity: mouse
application: immunocytochemistry, flow cytometry
citations: 6

Staining of mouse spleen cells with Rat anti Mouse CD8 ( MCA1108G )
quantity: 0.25 mg
price: 408 USD
to the supplier

Rat anti Mouse CD8 Alpha
Bio-Rad
catalog: MCA2694

rat monoclonal (53-6.7)
reactivity: mouse
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 4

Published customer image: . Rat anti Mouse CD8 antibody, clone 53-6.7 ( MCA2694 ) used to stain CD8 expressing tissue in mouse pancreatic sections by immunofluorescence. Image caption: . Determination of K b /B 22–29 -tetramer + CD8 T-cells in diabetic RIP-B7.1 tg mice. (C) The numbers of K b /B 22–29 -tetramer + CD8 T-cells were determined during the course of pCI/ppins A 12–21 -mediated EAD: group 1, health mice (n = 3) with blood glucose levels 200 mg/dl; group 2, early diabetic mice (n = 3) with blood glucose levels between 250-350 mg/dl; group 3, diabetic mice (n = 3) with severe diabetes (i.e., blood glucose levels between 400-550 mg/dl). Pancreata of representative mice out of groups 1 to 3 were analyzed histologically for CD8 T-cell influx (CD8 + ) or stained with hematoxylin-eosin (H&E). From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013). A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner. PLoS ONE 8(8): e71746 .
quantity: 0.25 mg
price: 408 USD
to the supplier

Rat anti Mouse CD8
Bio-Rad
catalog: MCA1768

rat monoclonal (YTS169.4)
reactivity: mouse
application: flow cytometry
citations: 2

Figure A. RPE conjugated Rat anti Mouse CD3 ( MCA500PE ) and FITC conjugated Rat IgG2b isotype control ( MCA6006F ). Figure B. RPE conjugated Rat anti Mouse CD3 ( MCA500PE ) and FITC conjugated Rat anti Mouse CD8 ( MCA1768F ). All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
quantity: 0.25 mg
price: 408 USD
to the supplier

Rat anti Mouse CD8 Alpha
Bio-Rad
catalog: MCA609GA

rat monoclonal (KT15)
reactivity: human, mouse
application: immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 3

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 0.1 mg
price: 272 USD
to the supplier

Rat anti Mouse CD8 Alpha
Bio-Rad
catalog: MCA609GT

rat monoclonal (KT15)
reactivity: human, mouse
application: immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 3

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 25 µg
price: 110 USD
to the supplier

Rat anti Mouse CD8 Alpha:RPE
Bio-Rad
catalog: MCA609PE

rat monoclonal (KT15)
reactivity: mouse
conjugate: RPE
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests
price: 428 USD
to the supplier

Rat anti Mouse CD8
Bio-Rad
catalog: MCA1108GT

rat monoclonal (YTS105.18)
reactivity: mouse
application: immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
citations: 1

Staining of mouse spleen cells with Rat anti Mouse CD8 ( MCA1108G )
quantity: 25 µg
price: 110 USD
to the supplier

Rat anti Mouse CD8
Bio-Rad
catalog: MCA1768T

rat monoclonal (YTS169.4)
reactivity: mouse
application: flow cytometry
citations: 1

Figure A. RPE conjugated Rat anti Mouse CD3 ( MCA500PE ) and FITC conjugated Rat IgG2b isotype control ( MCA6006F ). Figure B. RPE conjugated Rat anti Mouse CD3 ( MCA500PE ) and FITC conjugated Rat anti Mouse CD8 ( MCA1768F ). All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
quantity: 25 µg
price: 110 USD
to the supplier

Rat anti Mouse CD8 Alpha:Pacific Blue®
Bio-Rad
catalog: MCA609PBT

rat monoclonal (KT15)
reactivity: mouse
conjugate: Pacific Blue
application: flow cytometry
citations: 1

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 25 Tests/0.25 ml
price: 158 USD
to the supplier

Rat anti Mouse CD8 Alpha:APC
Bio-Rad
catalog: MCA609APC

rat monoclonal (KT15)
reactivity: mouse
conjugate: APC
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests
price: 428 USD
to the supplier

Rat anti Mouse CD8 Alpha:FITC
Bio-Rad
catalog: MCA609FB

rat monoclonal (KT15)
reactivity: mouse
conjugate: FITC
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 0.5 mg
price: 560 USD
to the supplier

Rat anti Mouse CD8 Alpha:FITC
Bio-Rad
catalog: MCA609F

rat monoclonal (KT15)
reactivity: mouse
conjugate: FITC
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 0.1 mg
price: 346 USD
to the supplier

Rat anti Mouse CD8 Alpha:Alexa Fluor® 647
Bio-Rad
catalog: MCA609A647

rat monoclonal (KT15)
reactivity: mouse
conjugate: AF647
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8 Alpha:RPE-Alexa Fluor® 647
Bio-Rad
catalog: MCA609P647

rat monoclonal (KT15)
reactivity: mouse
conjugate: RPE-Alexa Fluor 647
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8 Alpha:RPE-Alexa Fluor® 750
Bio-Rad
catalog: MCA609P750

rat monoclonal (KT15)
reactivity: mouse
conjugate: RPE
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8 Alpha:Pacific Blue®
Bio-Rad
catalog: MCA609PB

rat monoclonal (KT15)
reactivity: mouse
conjugate: Pacific Blue
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8:Low Endotoxin
Bio-Rad
catalog: MCA1108EL

rat monoclonal (YTS105.18)
reactivity: mouse
application: immunocytochemistry, flow cytometry

Staining of mouse spleen cells with Rat anti Mouse CD8 ( MCA1108G )
quantity: 0.5 mg
price: 679 USD
to the supplier

Rat anti Mouse CD8 Alpha:Alexa Fluor® 647
Bio-Rad
catalog: MCA609A647T

rat monoclonal (KT15)
reactivity: mouse
conjugate: AF647
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 25 Tests/0.25 ml
price: 158 USD
to the supplier

Rat anti Mouse CD8 Alpha
Bio-Rad
catalog: MCA2694T

rat monoclonal (53-6.7)
reactivity: mouse
application: immunocytochemistry, immunoprecipitation, flow cytometry

Published customer image: . Rat anti Mouse CD8 antibody, clone 53-6.7 ( MCA2694 ) used to stain CD8 expressing tissue in mouse pancreatic sections by immunofluorescence. Image caption: . Determination of K b /B 22–29 -tetramer + CD8 T-cells in diabetic RIP-B7.1 tg mice. (C) The numbers of K b /B 22–29 -tetramer + CD8 T-cells were determined during the course of pCI/ppins A 12–21 -mediated EAD: group 1, health mice (n = 3) with blood glucose levels 200 mg/dl; group 2, early diabetic mice (n = 3) with blood glucose levels between 250-350 mg/dl; group 3, diabetic mice (n = 3) with severe diabetes (i.e., blood glucose levels between 400-550 mg/dl). Pancreata of representative mice out of groups 1 to 3 were analyzed histologically for CD8 T-cell influx (CD8 + ) or stained with hematoxylin-eosin (H&E). From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013). A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner. PLoS ONE 8(8): e71746 .
quantity: 25 µg
price: 122 USD
to the supplier

Rat anti Mouse CD8 Alpha:APC
Bio-Rad
catalog: MCA609APCT

rat monoclonal (KT15)
reactivity: mouse
conjugate: APC
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 25 Tests
price: 158 USD
to the supplier

Rat anti Mouse CD8 Alpha:Alexa Fluor® 488
Bio-Rad
catalog: MCA609A488

rat monoclonal (KT15)
reactivity: mouse
conjugate: AF488
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8 Alpha:Alexa Fluor® 700
Bio-Rad
catalog: MCA609A700

rat monoclonal (KT15)
reactivity: mouse
conjugate: AF700
application: flow cytometry

Published customer image: . Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry. Image caption: . Peptide boost leads to loss of high avidity responses in vivo . A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFN elispot assay, D) normalization of responses to increasing peptide concentration in IFN elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments. From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112 .
quantity: 100 Tests/1 ml
price: 428 USD
to the supplier

Rat anti Mouse CD8:Alexa Fluor® 647
Bio-Rad
catalog: MCA1768A647

rat monoclonal (YTS169.4)
reactivity: mouse
conjugate: AF647
application: flow cytometry