domestic sheep polyclonal
reactivity: African green monkey, human, mouse
application: western blot, immunocytochemistry, other
citations: 22
reactivity: African green monkey, human, mouse
application: western blot, immunocytochemistry, other
citations: 22
Published customer image: . Sheep anti Human TGN46 antibody ( AHP500 ) used for the identification of the trans golgi network in MD-MB-231 cells by immunofluorescence. Image caption: . APP redistributes from endosomes to the TGN upon overexpression of μ4-F255A-HA. MD-MB-231 cells were cotransfected with a plasmid encoding either of the indicated HA-epitope-tagged variants of u4, and with a plasmid encoding APP-GFP carrying the double mutation F615P/D664A. After 24-h cells were fixed, permeabilized, stained for EEA1 and TGN46, and examined by fluorescence microscopy. Merging green, red, and blue channels generated the fourth image on each row; yellow indicates overlapping localization of the green and red channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of the red, green, and blue channels. Insets show 2× magnifications. Bar, 10 um. From: Citation: Ross BH, Lin Y, Corales EA, Burgos PV, Mardones GA (2014). Structural and Functional Characterization of Cargo-Binding Sites on the μ4-Subunit of Adaptor Protein Complex 4. PLoS ONE 9(2): e88147 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 25 µg
price: 789 USD
to the supplier
domestic sheep polyclonal
reactivity: African green monkey, human
application: western blot, immunocytochemistry, other
citations: 15
reactivity: African green monkey, human
application: western blot, immunocytochemistry, other
citations: 15
Published customer image: . Sheep anti Human TGN46 antibody ( AHP500 ) used for the identification of the trans golgi network in MD-MB-231 cells by immunofluorescence. Image caption: . APP redistributes from endosomes to the TGN upon overexpression of μ4-F255A-HA. MD-MB-231 cells were cotransfected with a plasmid encoding either of the indicated HA-epitope-tagged variants of u4, and with a plasmid encoding APP-GFP carrying the double mutation F615P/D664A. After 24-h cells were fixed, permeabilized, stained for EEA1 and TGN46, and examined by fluorescence microscopy. Merging green, red, and blue channels generated the fourth image on each row; yellow indicates overlapping localization of the green and red channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of the red, green, and blue channels. Insets show 2× magnifications. Bar, 10 um. From: Citation: Ross BH, Lin Y, Corales EA, Burgos PV, Mardones GA (2014). Structural and Functional Characterization of Cargo-Binding Sites on the μ4-Subunit of Adaptor Protein Complex 4. PLoS ONE 9(2): e88147 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 10 µg
price: 439 USD
to the supplier
domestic sheep polyclonal
reactivity: human
application: western blot, immunocytochemistry
citations: 14
reactivity: human
application: western blot, immunocytochemistry
citations: 14
Published customer image: . Sheep anti Human TGN46 antibody ( AHP500 ) used for the identification of the trans golgi network in MD-MB-231 cells by immunofluorescence. Image caption: . APP redistributes from endosomes to the TGN upon overexpression of μ4-F255A-HA. MD-MB-231 cells were cotransfected with a plasmid encoding either of the indicated HA-epitope-tagged variants of u4, and with a plasmid encoding APP-GFP carrying the double mutation F615P/D664A. After 24-h cells were fixed, permeabilized, stained for EEA1 and TGN46, and examined by fluorescence microscopy. Merging green, red, and blue channels generated the fourth image on each row; yellow indicates overlapping localization of the green and red channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of the red, green, and blue channels. Insets show 2× magnifications. Bar, 10 um. From: Citation: Ross BH, Lin Y, Corales EA, Burgos PV, Mardones GA (2014). Structural and Functional Characterization of Cargo-Binding Sites on the μ4-Subunit of Adaptor Protein Complex 4. PLoS ONE 9(2): e88147 . This image is from an open access article distributed under the terms of the Creative Commons Attribution License .
quantity: 0.1 ml
price: 439 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
citations: 1
reactivity: human
application: western blot, immunocytochemistry
citations: 1
Published customer image: . Rabbit anti Human TGN46 antibody used for the localisation of the trans golgi network in HeLa cells by immunofluorescence. Image caption: . Nuclear import of HPV16 vDNA is blocked in interphase cells. (A) Aphidicolin-treated or untreated HeLa cells were infected with HPV16-GFP. At 16 h p.i., cells were fixed and immunostained with the L1-7 antibody detecting endosomal, conformationally altered virions. Depicted are confocal sections of untreated (left) or aphidicolin-treated (right) cells. (B) As in (A) with quantification of the three-dimensional distance of L1-7 signals to the nuclear border (in µm) ± SD. (C) As in (A) with quantification of the number of discernible L1-7 spots/cell. (D) As in (A) with quantification of the fluorescent intensity (in arbitrary units, AU) of individual L1-7 spots. (E) HeLa H2B-mCherry cells were infected with BrdU-HPV16. Cells were immunostained for BrdU to detect the vDNA after fixation at 24 h p.i. Depicted are confocal sections of aphidicolin-treated (interphase, right) or untreated (left) cells. Mostly, the vDNA localized intranuclearly as discrete spots in untreated cells (arrowheads) or exclusively perinuclearly in aphidicolin-treated cells. (F) As in (E) with quantification of intranuclear BrdU spots/cell. The number of intranuclear spots is given relative to the total number of cellular spots. (G) As in (E) with quantification of perinuclear BrdU spot/cell as in (F). (H) As in (E) with quantification of the signal intensities of individual perinuclear BrdU spots relative to untreated infected cells. (I) HeLa cells were treated with or without aphidicolin for 16 h prior to infection. 20 h after infection with EdU-HPV16 (green), cells were fixed and immunostained for TGN46 (red) and LAMP1 (light blue). The nucleus was stained by Hoechst (dark blue). Depicted are single confocal sections. The cytosolic EdU-HPV16 signal is depicted after substraction of the EdU-HPV16 signals colocalizing with LAMP1, TGN46 and the nucleus. (J) As (I) with quantification of signal colocalization of EdU-HPV16 with LAMP1 or TGN46. Cytosolic EdU-HPV16 amounts were defined as signals that did not colocalize with LAMP1, TGN46, or the nucleus (see below). (K) As in (I) with quantification of signal colocalization of EdU-HPV16 with the nuclear stain (Hoechst). Statistical significance was determined by a two-tailed, independent t-test; P-values: * <0.05; *** <0.001. All scale bars: 10 µm. From: Aydin I, Weber S, Snijder B, Samperio Ventayol P, Kühbacher A, Becker M, et al. (2014) Large Scale RNAi Reveals the Requirement of Nuclear Envelope Breakdown for Nuclear Import of Human Papillomaviruses. PLoS Pathog 10(5): e1004162.
quantity: 0.1 ml
price: 542 USD
to the supplier
mouse monoclonal (2F11)
reactivity: human
reactivity: human